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http://dx.doi.org/10.11002/kjfp.2014.21.2.170

Rapid detection of the hepatitis a virus from fresh lettuce using immunomagnetic separation and quantum dots assay  

Lee, Hee-Min (Department of Food Science and Technology and Agribio Disaster Research Center)
Kwon, Joseph (Korea Basic Science Institute)
Choi, Jong-Soon (Korea Basic Science Institute)
Won, Yong-Gwan (Department of Electronics and Computer Engineering, Chonnam National University)
Kim, Eun-Sun (Health and Environment Institute)
Chung, Jae-Keun (Health and Environment Institute)
Kim, Min-Ji (Health and Environment Institute)
Kim, Duwoon (Department of Food Science and Technology and Agribio Disaster Research Center)
Publication Information
Food Science and Preservation / v.21, no.2, 2014 , pp. 170-174 More about this Journal
Abstract
Hepatitis A virus (HAV) infection leads to acute liver failure and death through the intake of contaminated food. The polymerase chain reaction (PCR) has been used to detect HAV in food samples. HAV detection takes a long time, however, due to the virus concentration step required before PCR assay. In this study, a rapid method of detecting the HAVs present in lettuce using immunomagnetic separation combined with quantum dots (IMS-QDs) assay was developed. The detection limit of IMS-QDs for HAV was 10 $TCID_{50}/mL$, similar to the result that was obtained using RT-PCR combined with PEG or IMS. The application of IMS-QDs assay completed the viral detection within one hour, but this was not possible using PEG combined with RT-PCR. In conclusion, IMS-QDs assay is a rapid and efficient method for detecting HAV at a low concentration in agricultural products.
Keywords
hepatitis A virus; immunomagnetic separation; quantum dot; RT-PCR;
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Times Cited By KSCI : 4  (Citation Analysis)
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