한국어병학회지 (Journal of fish pathology)

  • 제27권1호
  • /
  • Pages.47-56
  • /
  • 2014
  • /
  • 1226-0819(pISSN)
  • /
  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지
DOI QR코드

DOI QR Code

Propolis extracts가 양식넙치의 면역활성능에 미치는 영향

Effects of propolis extracts on the immune response in cultured flounder, Paralichthys olivaceus

  • 남현주 (군산대학교 해양과학대학 수산생명의학과) ;
  • 박경일 (군산대학교 해양과학대학 수산생명의학과) ;
  • 최민순 (군산대학교 해양과학대학 수산생명의학과)
  • Nam, Hyun Ju (Dept. of Aquatic Life Medicine, Kunsan National University) ;
  • Park, Kyung Il (Dept. of Aquatic Life Medicine, Kunsan National University) ;
  • Choi, Min Soon (Dept. of Aquatic Life Medicine, Kunsan National University)
  • 투고 : 2014.02.11
  • 심사 : 2014.04.09
  • 발행 : 2014.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Propolis extracts는 다양한 성분으로 구성되어 있는 수지상의 혼합물로서 여러 약리작용을 보여주기 때문에 민간요법으로 널리 이용되어왔다. 특히 propolis extracts는 독성이 거의 없다는 장점 때문에 면역증강제재로서 활용가능성이 높게 평가되고 있다. 이에 본 실험에서는 대상으로 in vitro 및 in vivo상에서 propolis extracts가 양식넙치 면역반응에 미치는 영향을 조사하였다. In vitro상에서 propolis extracts가 PBL의 탐식능 및 NBT assay활성능에 미치는 영향을 조사 한 바 유효농도는 $50{\sim}150{\mu}g/ml$ 이었으며, 가장 활성이 높은 농도는 $100{\mu}g/ml$ 이었다. In vivo상에서 양식넙치에 propolis extracts를 단독 또는 S. iniae 불활화 균액과 혼합하여 어체에 투여 후에 면역반응에 미치는 영향을 조사하였다. Haematocrit치는 propolis extracts단독 및 vaccine과 혼합 처리 할때 유의적 차이를 보이지 않았다. 그렇지만, 혈청 lysozyme과 신장대식세포의 탐식능, 활성산소 환원능 및 항체가는 propolis extracts+vaccine 투여구에서 유의적으로 증가하였다. 이러한 결과들에 의하여 propolis extracts는 양식넙치에서 특이적 및 비특이적 면역증강작용이 우수하므로 향후 양식어류에 있어서 비특이적 면역증강 물질 및 adjuvant로 활용가능성이 기대되어진다.

Propolis is a beehive product with a very complex chemical composition, widely used in folk medicine because of its several therapeutic activities. This study was conducted to measure the efficacy of propolis on non-specific defense reactions, specific immune response, and protection levels against pathogen challenge with Streptococcus iniae. in vitro and in vivo. In vitro, the phagocytic activity and NBT assay of peripheral blood leukocytes (PBL) were evaluated in a various propolis extractsconcentrations (0, 10, 50, 100, 150, 250 and $500{\mu}g/ml$). The optimal concentration showing activation of propolis extracts was determined to $100{\mu}g/ml$. In vivo, they were divided into four groups (PBS, propoli extractss, vaccine, propolis extracts + vaccine) in vivo. Fish were received i.p. injection of either PBS or propolis extracts, and in the presence or absence of formalin inactivated S. iniae ($1{\times}10^8$ CFU/fish), respectively. The level of haematocrit is not affected among experimental groups. The phagocytic activity and the NBT reduction activities of head kidney phagocyte were markedly (p<0.05)augmented in the propolis extracts groups than in the PBS-control group, respectively. The level of serum lysozyme activity was significantly (p<0.05) increased in the propolis extracts treated groups than in the PBS-control group. The agglutinin titer was significantly (p<0.05) enhanced in the vaccine+propolis extracts group than in the vaccine group, but there was no difference between PBS-control and propolis treated group. The results of the present study suggest that propolis extracts seems to be a promising compounds of non-specific immune stimulator, also being able to use a good adjuvant.

키워드

참고문헌

  1. Agnew W., Barnes A.C.: Streptococccus iniae : An aquatic pathogen of global veterinary significance and a challenging candidate for reliable vaccination. Vet. Microbiol,. 122: 1-15, 2007. https://doi.org/10.1016/j.vetmic.2007.03.002
  2. Anderson, D.P. , Moritomo, T. and de Grooth, R.: Neutrophil, glass-adherent, nitroblue tetrazolium assay gives early indication of immunization effectiveness in rainbow trout. Vet. Immunol. Immunopathol., 30: 419-429, 1992. https://doi.org/10.1016/0165-2427(92)90110-C
  3. Baeck G.W., Kim J. H., Gomez D.K. and Park S.C. : Isolation and characterization of Streptococcus sp. from diseased flounder(Paralichthys olivaceus) in Jeju island. J. Vet Sci. 7: 53-58, 2006. https://doi.org/10.4142/jvs.2006.7.1.53
  4. Bankova, V. : Chemical diversity of propolis the problem of standardization. J. Ethnophamacol. 100: 114-117, 2005a. https://doi.org/10.1016/j.jep.2005.05.004
  5. Bankova, V. : Recent trends and important developments in propolis research. ECAM. 2: 29-32. 2005b.
  6. Barton, B. B. and Iwama, G. K. : Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Ann. Rev. Fish Dis. 1: 3-26, 1991. https://doi.org/10.1016/0959-8030(91)90019-G
  7. Baulny, M. O., Quentel, C., Fournier, V., Lamour, F. and Gouvello, R. : Effect of long-term oral administration of $\beta$-glucan as an immunostimulant or an adjuvant on some non-specific parameters of the immune response of turbot Scophthalmus maximus. Dis. Aquat. Org., 26: 139-147, 1996. https://doi.org/10.3354/dao026139
  8. Burdock, G.A. : Review of the biological properties and toxicity of bee propolis (propolis). Food Chem. Toxicol. 36: 347-363, 1998. https://doi.org/10.1016/S0278-6915(97)00145-2
  9. Cetin, E., Kanbur M., Silici S., Erlasla G.: Propetamphosinduced changes in haematological and biochemical parameters of female rats: Protective role of propolis. Food Chem. Toxicol. 48: 1806-1810, 2010. https://doi.org/10.1016/j.fct.2010.04.010
  10. Cho M. Y., Lee J. S., Lee D.C., Choi H. J., and Kim J. W. : Immune response of olvive flounder Paralichthys olivaceus against $\beta$-haemolytic Streptococccus iniae formalin-killd cell. J. Fish. Pathol. 19: 73-82, 2006.
  11. Chu W. H. : Adjuvant effect of propolis on immunization by inactivated Aeromonas hydrophila in carp (Carassius auratus gibelio), Fish & Shellfish Immunol. 21: 113-117. 2006. https://doi.org/10.1016/j.fsi.2005.10.002
  12. Cuesta A., Rodriguez A., Esteban M. A and Meseguer J. : In vivo effects of propolis, a honeybee product, on gilthead seabream innate immune responses. Fish & Shellfish Immunol. 18: 71-80, 2005. https://doi.org/10.1016/j.fsi.2004.06.002
  13. Dobrovolski, J. W., Vohora, S. B., Sharma, K., Shah, S. A., Naqvi S. A. H. and Dandiya, P. C. : Antibacterial, antifungal, antiamoebic, antiinflammatory and antipyretic studies on propolis bee products. J. Ethnopharmacol. 35: 77-82, 1991. https://doi.org/10.1016/0378-8741(91)90135-Z
  14. Ellis, A. E. : Immunity to bacteria in fish. Fish & Shellfish Immunol., 9: 291-308, 1999. https://doi.org/10.1006/fsim.1998.0192
  15. Fan Y., Hu Y., Wang D., Guo Z., Zhao X., Guo L., Zhao B., Zhang J., Wan Y and Nguyen T.: Epimedium polysaccharide and propolis flavone can synergistically stimulate lymphocyte proliferation in vitro and enhance the immune responses to ND vaccine in chickens, Int. J. Biol. Macromol. 47: 87-92, 2010. https://doi.org/10.1016/j.ijbiomac.2010.05.017
  16. Fletcher, T. C. and White, A. : The lysozyme of the plaice pleuronecyes platessa L. Comp. Biochem. Physiol., 55: 207-210, 1996.
  17. Havsteen, B. : Flavonoids, a class of natural products of high pharmacological potency. Biochemical Pharmacology. 32: 1141-1148, 1983. https://doi.org/10.1016/0006-2952(83)90262-9
  18. Heo, G. J., Shin K. S. and Lee. M. H. : Diseases of aquaculture animals and prevention of drug residues. Kor. J. Food Hygiene, 7: S1-S19. 1992.
  19. Ingram, G. A. : Substances involved in the natural resistance of fish to infection a review. J. Fish Biol., 16: 23-60, 1992.
  20. Iwama, G., Pickering, A. D., Sumpter, J. P. and Schreck, C. B.: Fish stress and health in aquaculture. Cambridge University Press, 125-156, 1997.
  21. Jia, J. and Wu, Z : Effects of traditional Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker, Pseudomoscianea crocea(Richardson). Aquaculture 218: 1-9, 2003. https://doi.org/10.1016/S0044-8486(02)00192-8
  22. Kim H. J., Woo S. H., Kim J. W. and Park S.I. : Morphological characteristics and pathogenesity of Streptococcus iniae. J. Fish Pathol., 18: 167-178, 2005.
  23. Kim, J. W., Lee C. H., and Kim E. H. : Transferable R plasmid of streptococci sp. isolated from diseased olive flounder(Paralichthys olivaceus) in Jeju. J. Fish Pathol., 19: 267-276, 2006.
  24. Kim, J. W., Park, S. I. and Chun, S. K. : Purification and antibacterial effect of lysozyme from flounder, Paralichthys olivaceus. J. Fish Pathol., 5: 87-92, 1992.
  25. Kim, K. H., Hwang, Y. J. and Bai, S. C. : In vitro effect of aloe on the respiratory burst activity of olive flounder(Paralichthy olivaceus) leucocytes. J. Fish Pathol., 12: 1-6, 1999.
  26. Kim M. S., Seo. J. S., Park., M. A., Cho., J. M., Hwang., J. Y., Kwon., M. G., Jung., S. H.: Antimicrobial resistance of Edwardsiella tarda, Vibrio spp., Streptococcus spp. isolated from olive flounder Paralichthys olivaceous. J. Fish. Pathol. 23: 37-45, 2010.
  27. Nakanishi, T. and Ototake, M. : Antigen uptake and immune responses after immersion vaccination. In Fish Vaccinology, (ed. Cudding, R., Lillehaug, A., Midtlying, P.J. and Brown, F.), pp. 59-68, Karger Press, 1997.
  28. Nikl, L., T. P. T. Evelyn and L. J. Albrigt : Trials with an orally and immersion-administered Beta-1,3-glucan as an immunoprophylactic against Aeromo nas salmonicida in juvenile chinhook salmon Oncorhynchus tshwytscha. Dis. Aquat. Org., 17: 191-196. 1993. https://doi.org/10.3354/dao017191
  29. Roberson, B. S. : Bacterial agglutination. In techniques in fish immunology.(eds. Stolen J. S., Flecher, G. C., Anderson, D. P., Roberson, B. S. and van Muiswinkle W. B) pp. 137-154, Fair Haven(NJ): SOS publication; 1990.
  30. Sakai, M.: Current research status of fish immunostimulants. Aquaculture 172: 63-92, 1999. https://doi.org/10.1016/S0044-8486(98)00436-0
  31. Secombes, C. J. : Isolation of salmonid macrophages and analysis of their killing activity. In Techniques in fish immunology. (eds. Stolen J.S, Flecher G.C., anderson D.P., Roberson B.S. and van Muiswinkle W.B), pp. 137-154. Fair Haven (NJ): SOS publication, 1990,
  32. Secombes, C. J.: The nonspecific immune system: Cellular defences In: The fish immune system: Organism, pathogens, environment (eds . Iwama, G. and Nakanish, T.): pp.63-103, Academic Press, USA. 1996.
  33. Sforcin, J. M., Oris, R. O. and Bankova, V. : Effect of propolis, some isolated compounds and its source plant on antibody production. J. Enthnopharmacol. 98: 301-305, 2005. https://doi.org/10.1016/j.jep.2005.01.042
  34. Shon, Y. C. : Effects of medical herb stuff extracts and mitogens on the activation of kidney macrophage in nile tilapia, Oreochromis niloticus. Master Thesis, Pukyung National University, Korea, p63, 1999.
  35. Siwicki, A and Studnicka, M., : The phagocytic ability of neutrophils and serum lysozyme activity in experimentally infected carp, Cyprinus carpio L. J. Fish Biol., 31(Suppl A): 57-60, 1987. https://doi.org/10.1111/j.1095-8649.1987.tb05293.x
  36. Stahl, P. D. : The mannose receptor and other macrophages lectins. Cur. Opin. Immunol., 4: 49-52, 1992. https://doi.org/10.1016/0952-7915(92)90123-V
  37. Suzuki, Y., Otaka, T., Sato, S., Hou, Y. and Aida, K. : Reproduction related immunoglobulin changes in rainbow trout. Fish. Physiol. Biochem., 17 : 415-421, 1997. https://doi.org/10.1023/A:1007795827112
  38. Takahashi, Y., Itami, T. and Konegawa, K. : Enzymatic properties of partially lysozyme from the skin mucus of carp. Bull. Jap. Soc. Sci. Fish., 52: 1209-1214, 1986. https://doi.org/10.2331/suisan.52.1209

피인용 문헌

  1. 넙치(Paralichthys olivaceus)의 Streptococcus parauberis 인위감염을 위한 공격실험 방법에 관한 연구 vol.53, pp.4, 2014, https://doi.org/10.5657/kfas.2020.0628