Browse > Article
http://dx.doi.org/10.7847/jfp.2014.27.1.047

Effects of propolis extracts on the immune response in cultured flounder, Paralichthys olivaceus  

Nam, Hyun Ju (Dept. of Aquatic Life Medicine, Kunsan National University)
Park, Kyung Il (Dept. of Aquatic Life Medicine, Kunsan National University)
Choi, Min Soon (Dept. of Aquatic Life Medicine, Kunsan National University)
Publication Information
Journal of fish pathology / v.27, no.1, 2014 , pp. 47-56 More about this Journal
Abstract
Propolis is a beehive product with a very complex chemical composition, widely used in folk medicine because of its several therapeutic activities. This study was conducted to measure the efficacy of propolis on non-specific defense reactions, specific immune response, and protection levels against pathogen challenge with Streptococcus iniae. in vitro and in vivo. In vitro, the phagocytic activity and NBT assay of peripheral blood leukocytes (PBL) were evaluated in a various propolis extractsconcentrations (0, 10, 50, 100, 150, 250 and $500{\mu}g/ml$). The optimal concentration showing activation of propolis extracts was determined to $100{\mu}g/ml$. In vivo, they were divided into four groups (PBS, propoli extractss, vaccine, propolis extracts + vaccine) in vivo. Fish were received i.p. injection of either PBS or propolis extracts, and in the presence or absence of formalin inactivated S. iniae ($1{\times}10^8$ CFU/fish), respectively. The level of haematocrit is not affected among experimental groups. The phagocytic activity and the NBT reduction activities of head kidney phagocyte were markedly (p<0.05)augmented in the propolis extracts groups than in the PBS-control group, respectively. The level of serum lysozyme activity was significantly (p<0.05) increased in the propolis extracts treated groups than in the PBS-control group. The agglutinin titer was significantly (p<0.05) enhanced in the vaccine+propolis extracts group than in the vaccine group, but there was no difference between PBS-control and propolis treated group. The results of the present study suggest that propolis extracts seems to be a promising compounds of non-specific immune stimulator, also being able to use a good adjuvant.
Keywords
propolis; NBT; phagocytosis; lysozyme; antibody;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Secombes, C. J.: The nonspecific immune system: Cellular defences In: The fish immune system: Organism, pathogens, environment (eds . Iwama, G. and Nakanish, T.): pp.63-103, Academic Press, USA. 1996.
2 Sforcin, J. M., Oris, R. O. and Bankova, V. : Effect of propolis, some isolated compounds and its source plant on antibody production. J. Enthnopharmacol. 98: 301-305, 2005.   DOI   ScienceOn
3 Shon, Y. C. : Effects of medical herb stuff extracts and mitogens on the activation of kidney macrophage in nile tilapia, Oreochromis niloticus. Master Thesis, Pukyung National University, Korea, p63, 1999.
4 Stahl, P. D. : The mannose receptor and other macrophages lectins. Cur. Opin. Immunol., 4: 49-52, 1992.   DOI   ScienceOn
5 Suzuki, Y., Otaka, T., Sato, S., Hou, Y. and Aida, K. : Reproduction related immunoglobulin changes in rainbow trout. Fish. Physiol. Biochem., 17 : 415-421, 1997.   DOI
6 Takahashi, Y., Itami, T. and Konegawa, K. : Enzymatic properties of partially lysozyme from the skin mucus of carp. Bull. Jap. Soc. Sci. Fish., 52: 1209-1214, 1986.   DOI
7 Fletcher, T. C. and White, A. : The lysozyme of the plaice pleuronecyes platessa L. Comp. Biochem. Physiol., 55: 207-210, 1996.
8 Havsteen, B. : Flavonoids, a class of natural products of high pharmacological potency. Biochemical Pharmacology. 32: 1141-1148, 1983.   DOI   ScienceOn
9 Heo, G. J., Shin K. S. and Lee. M. H. : Diseases of aquaculture animals and prevention of drug residues. Kor. J. Food Hygiene, 7: S1-S19. 1992.
10 Ingram, G. A. : Substances involved in the natural resistance of fish to infection a review. J. Fish Biol., 16: 23-60, 1992.
11 Iwama, G., Pickering, A. D., Sumpter, J. P. and Schreck, C. B.: Fish stress and health in aquaculture. Cambridge University Press, 125-156, 1997.
12 Kim, J. W., Park, S. I. and Chun, S. K. : Purification and antibacterial effect of lysozyme from flounder, Paralichthys olivaceus. J. Fish Pathol., 5: 87-92, 1992.
13 Jia, J. and Wu, Z : Effects of traditional Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker, Pseudomoscianea crocea(Richardson). Aquaculture 218: 1-9, 2003.   DOI   ScienceOn
14 Kim H. J., Woo S. H., Kim J. W. and Park S.I. : Morphological characteristics and pathogenesity of Streptococcus iniae. J. Fish Pathol., 18: 167-178, 2005.
15 Nakanishi, T. and Ototake, M. : Antigen uptake and immune responses after immersion vaccination. In Fish Vaccinology, (ed. Cudding, R., Lillehaug, A., Midtlying, P.J. and Brown, F.), pp. 59-68, Karger Press, 1997.
16 Kim, J. W., Lee C. H., and Kim E. H. : Transferable R plasmid of streptococci sp. isolated from diseased olive flounder(Paralichthys olivaceus) in Jeju. J. Fish Pathol., 19: 267-276, 2006.
17 Kim, K. H., Hwang, Y. J. and Bai, S. C. : In vitro effect of aloe on the respiratory burst activity of olive flounder(Paralichthy olivaceus) leucocytes. J. Fish Pathol., 12: 1-6, 1999.   과학기술학회마을
18 Kim M. S., Seo. J. S., Park., M. A., Cho., J. M., Hwang., J. Y., Kwon., M. G., Jung., S. H.: Antimicrobial resistance of Edwardsiella tarda, Vibrio spp., Streptococcus spp. isolated from olive flounder Paralichthys olivaceous. J. Fish. Pathol. 23: 37-45, 2010.   과학기술학회마을
19 Nikl, L., T. P. T. Evelyn and L. J. Albrigt : Trials with an orally and immersion-administered Beta-1,3-glucan as an immunoprophylactic against Aeromo nas salmonicida in juvenile chinhook salmon Oncorhynchus tshwytscha. Dis. Aquat. Org., 17: 191-196. 1993.   DOI
20 Roberson, B. S. : Bacterial agglutination. In techniques in fish immunology.(eds. Stolen J. S., Flecher, G. C., Anderson, D. P., Roberson, B. S. and van Muiswinkle W. B) pp. 137-154, Fair Haven(NJ): SOS publication; 1990.
21 Sakai, M.: Current research status of fish immunostimulants. Aquaculture 172: 63-92, 1999.   DOI
22 Anderson, D.P. , Moritomo, T. and de Grooth, R.: Neutrophil, glass-adherent, nitroblue tetrazolium assay gives early indication of immunization effectiveness in rainbow trout. Vet. Immunol. Immunopathol., 30: 419-429, 1992.   DOI   ScienceOn
23 Barton, B. B. and Iwama, G. K. : Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Ann. Rev. Fish Dis. 1: 3-26, 1991.   DOI
24 Baeck G.W., Kim J. H., Gomez D.K. and Park S.C. : Isolation and characterization of Streptococcus sp. from diseased flounder(Paralichthys olivaceus) in Jeju island. J. Vet Sci. 7: 53-58, 2006.   과학기술학회마을   DOI   ScienceOn
25 Agnew W., Barnes A.C.: Streptococccus iniae : An aquatic pathogen of global veterinary significance and a challenging candidate for reliable vaccination. Vet. Microbiol,. 122: 1-15, 2007.   DOI   ScienceOn
26 Bankova, V. : Recent trends and important developments in propolis research. ECAM. 2: 29-32. 2005b.
27 Baulny, M. O., Quentel, C., Fournier, V., Lamour, F. and Gouvello, R. : Effect of long-term oral administration of $\beta$-glucan as an immunostimulant or an adjuvant on some non-specific parameters of the immune response of turbot Scophthalmus maximus. Dis. Aquat. Org., 26: 139-147, 1996.   DOI   ScienceOn
28 Burdock, G.A. : Review of the biological properties and toxicity of bee propolis (propolis). Food Chem. Toxicol. 36: 347-363, 1998.   DOI   ScienceOn
29 Cetin, E., Kanbur M., Silici S., Erlasla G.: Propetamphosinduced changes in haematological and biochemical parameters of female rats: Protective role of propolis. Food Chem. Toxicol. 48: 1806-1810, 2010.   DOI
30 Dobrovolski, J. W., Vohora, S. B., Sharma, K., Shah, S. A., Naqvi S. A. H. and Dandiya, P. C. : Antibacterial, antifungal, antiamoebic, antiinflammatory and antipyretic studies on propolis bee products. J. Ethnopharmacol. 35: 77-82, 1991.   DOI   ScienceOn
31 Ellis, A. E. : Immunity to bacteria in fish. Fish & Shellfish Immunol., 9: 291-308, 1999.   DOI   ScienceOn
32 Cho M. Y., Lee J. S., Lee D.C., Choi H. J., and Kim J. W. : Immune response of olvive flounder Paralichthys olivaceus against $\beta$-haemolytic Streptococccus iniae formalin-killd cell. J. Fish. Pathol. 19: 73-82, 2006.
33 Chu W. H. : Adjuvant effect of propolis on immunization by inactivated Aeromonas hydrophila in carp (Carassius auratus gibelio), Fish & Shellfish Immunol. 21: 113-117. 2006.   DOI   ScienceOn
34 Cuesta A., Rodriguez A., Esteban M. A and Meseguer J. : In vivo effects of propolis, a honeybee product, on gilthead seabream innate immune responses. Fish & Shellfish Immunol. 18: 71-80, 2005.   DOI   ScienceOn
35 Fan Y., Hu Y., Wang D., Guo Z., Zhao X., Guo L., Zhao B., Zhang J., Wan Y and Nguyen T.: Epimedium polysaccharide and propolis flavone can synergistically stimulate lymphocyte proliferation in vitro and enhance the immune responses to ND vaccine in chickens, Int. J. Biol. Macromol. 47: 87-92, 2010.   DOI   ScienceOn
36 Secombes, C. J. : Isolation of salmonid macrophages and analysis of their killing activity. In Techniques in fish immunology. (eds. Stolen J.S, Flecher G.C., anderson D.P., Roberson B.S. and van Muiswinkle W.B), pp. 137-154. Fair Haven (NJ): SOS publication, 1990,
37 Siwicki, A and Studnicka, M., : The phagocytic ability of neutrophils and serum lysozyme activity in experimentally infected carp, Cyprinus carpio L. J. Fish Biol., 31(Suppl A): 57-60, 1987.   DOI
38 Bankova, V. : Chemical diversity of propolis the problem of standardization. J. Ethnophamacol. 100: 114-117, 2005a.   DOI   ScienceOn