Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Anti-Obesity Effects of Lentinus edodes on Obese Mice Induced by High Fat Diet

표고버섯이 고지방식이로 유도한 비만 흰쥐에 미치는 항비만 효과

  • Lee, Mi Ra (Dept. of Food Science and Technology, Chungnam National University) ;
  • Oh, Deuk Sil (Jeollanam-do Forest Resource Research Institute) ;
  • Wee, An Jin (Jeollanam-do Forest Resource Research Institute) ;
  • Yun, Byung Sun (Jeollanam-do Forest Resource Research Institute) ;
  • Jang, Soon Ae (Hanbat University Industry Collaboration Foundation) ;
  • Sung, Chang Keun (Dept. of Food Science and Technology, Chungnam National University)
  • Received : 2013.09.27
  • Accepted : 2013.10.24
  • Published : 2014.02.28
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Abstract

The aim of this study was to investigate the anti-obesity effects of Lentinus edodes water extract powder (LEP) in mice fed a high fat diet (HF, 45% kcal fat). Mice were administrated a HF diet supplemented with 1%, 3%, or 5% LEP for 12 weeks. Consumption of HF diet caused increases in body weight, serum lipid profiles, and adipose tissue weights. Serum TC and TG levels in the LEP-supplemented groups were lower than those in the NC group. Supplementation with 5% LEP significantly suppressed body weight gain and reduced the weight of subcutaneous adipose tissue compared to the HF group. HF diet ingestion resulted in higher lipid content and increased lipid peroxidation in the liver. However, LEP supplementation inhibited accumulation of hepatic lipids induced by HF diet, considerably decreased MDA levels, and elevated total antioxidant activity in the livers of mice in the 5% LEP group. Histopathological analysis indicated that the livers of mice fed HF diet developed hepatic steatosis, whereas LEP-treated groups showed small fat droplets. These results suggest that long-term supplementation with LEP may also have an ameliorating effect on HF-induced obesity.

본 연구는 표고버섯 물추출물의 항비만 효과를 알아보기 위하여 6주령의 수컷 C57BL/6 마우스를 이용하여 고지방식이와 함께 표고버섯 물추출 분말을 1%, 3%, 5% 수준으로 첨가하여 12주간 공급하였다. 고지방 식이섭취는 체중, 혈청지질 농도 및 지방조직 무게를 증가시켰다. 표고버섯 물추출물 섭취는 고지방식이로 증가된 혈청 총콜레스테롤, 중성지방, LDL-콜레스테롤 농도를 현저히 감소시켜 대조군보다 더 낮은 값을 나타냈다. 또한 5% 표고버섯 물추출물 첨가군은 고지방 식이군보다 체중 및 피하지방량이 현저히 감소하였다. 변배설량은 3%와 5% 표고버섯 물추출물 첨가군에서 고지방 식이군보다 현저히 증가하였다. 표고버섯 물추출물 섭취는 고지방식이로 인해 증가된 간조직의 지방축적과 지질과산화물 형성을 억제하였으며, 특히 5% 표고버섯 물추출물 섭취군에서 지질과산화물 형성이 유의적으로 감소되었고 총 항산화 활성도 유의적으로 가장 높게 나타났다. 고지방식이군은 간조직의 조직염색에서 지방간이 심하게 진행된 것이 발견되었고, 표고버섯 물추출물 섭취군은 지방세포에 지방이 침투하지 않은 간지방증 소견을 보였다. 위의 결과를 통하여 볼 때 표고버섯은 체중 및 체지방 감소, 그리고 혈청지방 농도 개선에 효과가 있어 비만관리에 도움을 주는 것으로 사료된다.

Keywords

References

  1. Bray GA. 2000. A concise review on the therapeutics of obesity. Nutrition 16: 953-960. https://doi.org/10.1016/S0899-9007(00)00424-X
  2. Leonhardt M, Hrupka B, Langhans W. 1999. New approaches in the pharmacological treatment of obesity. Eur J Nutr 38: 1-13. https://doi.org/10.1007/s003940050040
  3. WHO. 10 facts on obesity. http://www.who.int/features/factfiles/ obesity/en/index.html.
  4. Kopelman PG. 2000. Obesity as a medical problem. Nature 404: 635-643.
  5. Yao F, MacKenzie RG. 2010. Obesity drug update: The lost decade? Pharmaceuticals 3: 3494-3521. https://doi.org/10.3390/ph3123494
  6. Jahromi F, Ray AB, Chansouria JPN. 1993. Antihyperlipidemic effect of flavonoids from Pterocarpus marsupium. J Nat Prod 56: 989-994. https://doi.org/10.1021/np50097a001
  7. Molitoris HP. 1994. Mushrooms in medicine. Folia Microbiol 39: 91-98. https://doi.org/10.1007/BF02906801
  8. Wasser SP. 2002. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol 60: 258-274. https://doi.org/10.1007/s00253-002-1076-7
  9. Surenjav U, Zhang L, Xu X, Zhang X, Zeng F. 2006. Effects of molecular structure on antitumor activities of (1$\rightarrow$3)-$\beta$- d-glucans from different Lentinus edodes. Carbohyd Polym 63: 97-104. https://doi.org/10.1016/j.carbpol.2005.08.011
  10. Yang BK, Kim DH, Jeong SC, Das S, Choi YS, Shin JS, Lee SC, Song CH. 2002. Hypoglycemic effect of a Lentinus edodes exo-polymer produced from a submerged mycelial culture. Biosci Biotechnol Biochem 66: 937-942. https://doi.org/10.1271/bbb.66.937
  11. Kabir Y, Yamaguchi M, Kimura S. 1987. Effect of shiitake (Lentinus edodes) and maitake (Grifola frondosa) mushrooms on blood pressure and plasma lipids of spontaneously hypertensive rats. J Nutr Sci Vitaminol 33: 341-346. https://doi.org/10.3177/jnsv.33.341
  12. Ng ML, Yap AT. 2002. Inhibition of human colon carcinoma development by lentinan from shiitake mushrooms (Lentinus edodes). J Altern Complement Med 8: 581-589. https://doi.org/10.1089/107555302320825093
  13. Enman J, Rova U, Berglund KA. 2007. Quantification of the bioactive compound eritadenine in selected strains of shiitake mushroom (Lentinus edodes). J Agric Food Chem 55: 1177-1180. https://doi.org/10.1021/jf062559+
  14. Hatvani N. 2001. Antibacterial effect of the culture fluid of Lentinus edodes mycelium grown in submerged liquid culture. Int J Antimicrob Agents 17: 71-74. https://doi.org/10.1016/S0924-8579(00)00311-3
  15. Kim HS, Jo YC, Lee YJ, Park IB, Park JW, Jung MA, Kim YS, Kim SO. 2013. Inhibitory effects of Lentinus edodes and rice with Lentinus edodes mycelium on diabetes and obesity. J Korean Soc Food Sci Nutr 42: 175-181. https://doi.org/10.3746/jkfn.2013.42.2.175
  16. Brennan CS, Cleary LJ. 2005. The potential use of cereal (1-3,1-4)-$\beta$-D-glucans as functional food ingredients. J Cereal Sci 42: 1-13. https://doi.org/10.1016/j.jcs.2005.01.002
  17. Sharma AM. 2002. Adipose tissue: a mediator of cardiovascular risk. Int J Obes Relat Metab Disord 4: S5-S7.
  18. Choi EH, Yang HP, Chun HS. 2012. Chitooligosaccharide ameliorates diet-induced obesity in mice and affects adipose gene expression involved in adipogenesis and inflammation. Nutr Res 32: 218-228. https://doi.org/10.1016/j.nutres.2012.02.004
  19. Chandra LC, Smith BJ, Clarke SL, Marlow D, Offay JM, Kuvibidila SR. 2011. Differential effects of shiitake- and white button mushroom-supplemented diets on hepatic steatosis in C57BL/6 mice. Food Chem Toxicol 49: 3074-3080. https://doi.org/10.1016/j.fct.2011.09.001
  20. Folch J, Lees M, Sloane-Stanley GH. 1957. A simple method for the isolation and purification of total lipids from animal tissue. J Biol Chem 226: 497-509.
  21. Friedwald WT, Levy RL, Fredrickson DS. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18: 499-502.
  22. Uchiyama M, Mihara M. 1978. Determination of malonaldehyde precursor in tissues by thiobarbituric acid. Anal Biochem 86: 271-278. https://doi.org/10.1016/0003-2697(78)90342-1
  23. Pittler MH, Ernst E. 2001. Guar gum for body weight reduction: meta-analysis of randomized trials. Am J Med 110: 724-730. https://doi.org/10.1016/S0002-9343(01)00702-1
  24. Bhandari U, Chaudhari HS, Ajay Narayan Bisnoi AN, Kumar V, Khanna G, Javed K. 2013. Anti-obesity effect of standardized ethanol extract of Embelia ribes in murine model of high fat diet-induced obesity. Pharma Nutrition 1: 50-57. https://doi.org/10.1016/j.phanu.2013.01.001
  25. Yamada T, Oinuma T, Niihashi M. 2002. Effects of Lentinus edodes mycelia on dietary-induced atherosclerotic involvement in rabbit aorta. J Atheroscler Thromb 9: 149-156. https://doi.org/10.5551/jat.9.149
  26. Sugiyama K, Akachi T, Yamakawa A. 1995. Eritadenine-induced alteration of hepatic phospholipid metabolism in relation to its hypocholesterolemic action in rats. J Nutr Biochem 6: 80-87. https://doi.org/10.1016/0955-2863(94)00017-G
  27. Lee JS, Lee MK, Ha TY, Bok SH, Park HM, Jeong KS, Woo MN, Do GM, Yeo JY, Choi MS. 2006. Supplementation of whole persimmon leaf improves lipid profiles and suppresses body weight gain in rats fed high-fat diet. Food Chem Toxicol 44: 1875-1883. https://doi.org/10.1016/j.fct.2006.06.014
  28. Chau CF, Huang YL, Lin CY. 2004. Investigation of the cholesterol-lowering action of insoluble fibre derived from the peel of Citrus sinenesis L. cv. Liucheng. Food Chem 87: 361-366. https://doi.org/10.1016/j.foodchem.2003.12.006
  29. Hasty HA, Gruen ML, Terry ES, Surmi BK, Atkinson RD, Gao L, Morrow JD. 2007. Effects of vitamin E on oxidative stress and atherosclerosis in an obese hyperlipidemic mouse model. J Nutr Biochem 18: 127-133. https://doi.org/10.1016/j.jnutbio.2006.03.012
  30. Xu C, Yan ZH, Hong ZJ, Jing G. 2008. The pharmacological effect of polysaccharides from Lentinus edodes on the oxidative status and expression of VCAM-1mRNA of thoracic aorta endothelial cell in high-fat-diet rats. Carbohyd Polym 74: 445-450. https://doi.org/10.1016/j.carbpol.2008.03.018
  31. Sugiyama K, Yamakawa A. 1996. Dietary eritadenine-induced alteration of molecular species composition of phospholipids in rats. Lipids 31: 399-404. https://doi.org/10.1007/BF02522926

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