Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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In vitro anti-cancer activity of hydrophobic fractions of Sparassis latifolia extract using AGS, A529, and HepG2 cell lines

꽃송이버섯(Sparassis latifolia) 추출물 소수성 분획의 항암 활성

  • Choi, Moon-Hee (Department of Chemical Engineering, Graduate School of Engineering, Chosun University) ;
  • Han, Hyo-Kyung (College of Pharmacy, Dongguk University) ;
  • Lee, Yong-Jo (Department of Chemical Engineering, Graduate School of Engineering, Chosun University) ;
  • Jo, Han-Gyo (Department of Chemical Engineering, Graduate School of Engineering, Chosun University) ;
  • Shin, Hyun-Jae (Department of Chemical Engineering, Graduate School of Engineering, Chosun University)
  • 최문희 (조선대학교 대학원 화학공학과) ;
  • 한효경 (동국대학교 약학대학 약학과) ;
  • 이용조 (조선대학교 대학원 화학공학과) ;
  • 조한교 (조선대학교 대학원 화학공학과) ;
  • 신현재 (조선대학교 대학원 화학공학과)
  • Received : 2014.11.03
  • Accepted : 2014.12.17
  • Published : 2014.12.31
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Abstract

The use of mushrooms has immense potential in many diverse applications. Until now, more than 3,000 species are consumed around the world, and more than 100 have shown promising clinical activity against cancer and other chronic diseases. Sparassis latifolia (formerly S. crispa) is an edible mushroom that harbors ${\beta}$-glucan reported to possess immunostimulatory and anticancer properties. However there have been no reports on the anticancer activity of hydrophobic fractions of S. latifolia. In this study, the anticancer activities of S. latifolia extract and hydrophobic fractions were investigated using AGS (stomach cancer), A529 (lung cancer), and HepG2 (liver cancer) cell lines. In cytotoxicity results of A529 cells, fractions of A2, A3, A4, A6, A7, A8, A9, and A10 in all 12 fractions show low $IC_{50}$ values. For HepG2 cells, A7 fraction results in the lowest $IC_{50}$ value while A7, A8, and A11 fractions show low $IC_{50}$ values in AGS cells. S. latifolia extract lead to low cell viability in cancer cells, compared to positive control of paclitaxel. A compound with molecular weight of 181 were detected using HPLC-MS but not identified yet. As a result, the hydrophobic fractions of S. latifolia EtOH extract would be a possible candidate as natural anticancer agents in the future.

본 실험에서는 꽃송이버섯 (Sparassis crispa, formerly S. crispa) 에탄올 추출물의 소수성 분획을 분리하고 각 분획의 DPPH 항산화 활성과 위암 (AGS), 폐암 (A529), 간암 (HepG2) 세포주를 이용한 항암 활성을 MTT assay를 통해 확인 하였다. SOCC를 사용하여 총 18개의 fraction으로 분획하였고 TLC와 세포주를 이용한 항암 활성 확인을 통하여 5개의 fraction으로 압축하였다. 항암활성이 높은 5개의 fraction은 HPLC-MS를 통해 각 분획물을 분석한 결과 항산화 활성을 보이지 않았으며 약 181.0의 분자량을 가진 물질이 지표물질로 확인되었으므로 이 물질의 화학식 동정을 위하여 추가실험이 필요하다. 세포주를 이용한 항암실험 결과 꽃송이버섯 추출물은 위암 (AGS), 폐암 (A529), 간암 (HepG2) 세포주 모두에서 양성대조군인 paclitaxel보다 낮은 세포 생존율을 보여 주었으며($IC_{50}$ value), 이것은 추후 꽃송이버섯 추출물에 포함된 항암 물질 분리 연구를 위한 기초연구 결과로서 무척 의미가 크다고 할 수 있다.

Keywords

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