한국균학회지 (The Korean Journal of Mycology)

  • 제42권4호
  • /
  • Pages.312-321
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  • 2014
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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한국산 가송이(가칭)의 균사생장 특성과 소나무에 외생균근 형성

Mycelial Growth and in vitro Ectomycorrhizal Synthesis on Pinus densiflora Seedlings of Tricholoma bakamatsutake in Korea

  • 전성민 (국립산림과학원 화학미생물과) ;
  • 가강현 (국립산림과학원 화학미생물과) ;
  • 홍기성 (산림조합중앙회 산림버섯연구센터)
  • Jeon, Sung-Min (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Ka, Kang-Hyeon (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Hong, Ki-Sung (Forest Mushroom Research Center, National Forestry Cooperative Federation)
  • 투고 : 2014.11.29
  • 심사 : 2014.12.09
  • 발행 : 2014.12.30
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초록

가송이(Tricholoma bakamatsutake)는 송이(Tricholoma matsutake)의 근연종으로 식용 가능한 외생균근성 버섯 중의 하나이다. 본 연구는 우리나라 신갈나무림에서 수집한 가송이 균주들의 생리학적 특성에 관한 최초의 보고로, 자실체의 조직으로부터 순수 분리한 균주를 대상으로 다양한 조건(배지, 온도, 질소원)에서 이들의 배양 특성을 조사하였다. 대부분의 균주들은 PDA 배지, 20 또는 $25^{\circ}C$의 배양온도에서 균 생장력이 높았으며, 시험균 중 2균주는 그들의 균사생장에 질산염보다 암모늄 형태의 무기질소원을 더 선호하였다. 가송이는 근연종인 송이와 최적배양 조건이 유사하기는 하나, 균사생장 속도가 송이보다 더 느림을 알 수 있었다. 가송이의 모든 균주들은 carboxymethylcellulose 배지 상에서 cellulase 활성을 나타냈다. 또한 가송이 2균주는 소나무 실생묘에 이들 균을 접종한 지 3 또는 8개월 후 균근을 각각 형성하였으며, 균을 접종하지 않은 묘목보다 균을 접종한 묘목의 생체량이 더 큰 것으로 조사되었다.

Tricholoma bakamatsutake is one of the edible ectomycorrhizal mushrooms as an allied species of Tricholoma matsutake. This is the first report on physical characteristics of T. bakamatsutake strains collected from Quercus mongolica forests in Korea. The pure cultures of these strains were isolated from the tissues of fruit bodies and the culture characteristics were investigated under different conditions (media, temperatures, nitrogen sources). Most strains showed the highest mycelial growth on potato dextrose agar (PDA) at 20 or $25^{\circ}C$. Two strains of T. bakamatsutake preferred the ammonium-form rather than the nitrate-form as an inorganic nitrogen source. T. bakamatsutake showed significantly slower mycelial growth when compared with T. matsutake from a Korean forest, although the optimum culture conditions for the two allied species were similar. We also tested the ability to form mycorrhizae as well as cellulase activity of T. bakamatsutake. All strains showed cellulase activity on a carboxymethylcellulose (CMC) agar plate. The mycorrhizae on axenic Pinus densiflora seedlings were formed by two strains of T. bakamatsutake after 3 or 8 months of inoculation. P. densiflora seedlings inoculated with T. bakamatsutake had a much higher biomass than un-inoculated seedlings.

키워드

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피인용 문헌

  1. First Record of Mattirolomyces terfezioides and Tricholoma bakamatsutake in Korea vol.43, pp.2, 2015, https://doi.org/10.4489/KJM.2015.43.2.125
  2. Cultural Characteristics of Korean Ectomycorrhizal Fungi vol.43, pp.1, 2015, https://doi.org/10.4489/KJM.2015.43.1.1