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http://dx.doi.org/10.4489/KJM.2014.42.4.312

Mycelial Growth and in vitro Ectomycorrhizal Synthesis on Pinus densiflora Seedlings of Tricholoma bakamatsutake in Korea  

Jeon, Sung-Min (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute)
Ka, Kang-Hyeon (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute)
Hong, Ki-Sung (Forest Mushroom Research Center, National Forestry Cooperative Federation)
Publication Information
The Korean Journal of Mycology / v.42, no.4, 2014 , pp. 312-321 More about this Journal
Abstract
Tricholoma bakamatsutake is one of the edible ectomycorrhizal mushrooms as an allied species of Tricholoma matsutake. This is the first report on physical characteristics of T. bakamatsutake strains collected from Quercus mongolica forests in Korea. The pure cultures of these strains were isolated from the tissues of fruit bodies and the culture characteristics were investigated under different conditions (media, temperatures, nitrogen sources). Most strains showed the highest mycelial growth on potato dextrose agar (PDA) at 20 or $25^{\circ}C$. Two strains of T. bakamatsutake preferred the ammonium-form rather than the nitrate-form as an inorganic nitrogen source. T. bakamatsutake showed significantly slower mycelial growth when compared with T. matsutake from a Korean forest, although the optimum culture conditions for the two allied species were similar. We also tested the ability to form mycorrhizae as well as cellulase activity of T. bakamatsutake. All strains showed cellulase activity on a carboxymethylcellulose (CMC) agar plate. The mycorrhizae on axenic Pinus densiflora seedlings were formed by two strains of T. bakamatsutake after 3 or 8 months of inoculation. P. densiflora seedlings inoculated with T. bakamatsutake had a much higher biomass than un-inoculated seedlings.
Keywords
Cellulase; Ectomycorrhizal synthesis; Mycelial growth; Pinus densiflora; Tricholoma bakamatsutake;
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1 Index Fungorum. Search Index fongorum [Intenet]. Index Fungorum Partnership; 2014. Available from http://www.indexfungorum.org/Names/NamesRecord.asp?RecordID=324912.
2 Ohta A. Ability of ectomycorrhizal fungi to utilize starch and related substrates. Mycoscience 1997;38:403-8.   DOI
3 Hosford D, David P, Randy M, Michael A. Ecology and management of the commercially harvested American matsutake mushroom. Gen. Tech. Rep. PNW-GTR-412. Portland, OR: U.S. Department of Agriculture, Forest Service, Pacific Northwest Research Station; 1997.
4 Yamada T. Isolation of Tricholoma matsutake and T. bakamatsutake cultures from collected filed-collected ectomycorrhizas. Mycoscience 2001;42:43-50.   DOI   ScienceOn
5 Wills RM, Lipsey RG. An economic strategy to develop nontimber forest products and services in British Columbia. Final Report; Forest Renewal BC Project No. PA97538-ORE. Cognetics International Research Inc.; 1999.
6 Yamada A. Utility of mycorrhizal mushrooms as food resources in Japan. J Fac Agric Shinshu Univ 2002;38:1-17.
7 American Type Culture Collection (ATCC); 2014 Available from http://www.atcc.org/
8 Terashima Y. Change in medium components and colony morphology due to mycelial growth of ectomycorrhizal fungus Tricholoma bakamatsutake. Mycoscience 1994;35:153-9.   DOI
9 Terashima Y. Carbon and nitrogen utilization and acid production by media of the ectomycorrhizal fungus Tricholoma bakamatsutake in vitro. Mycoscience 1999;40:51-6.   DOI   ScienceOn
10 Yamanaka T. Researches for development of the cultivation of matsutake, a prized mushroom produced by the ectomycorrhizal basidiomycete Tricholoma matsutake. Bulletin of FFPRI 2012;11:85-95.
11 Zimbro MJ, Power DA. Manual of microbiological culture media. Sparks: Becton Dickinson and Company; 2003.
12 Marx DH. The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria. Phytopathology 1969;59:153-63.
13 Jeon SM, Ka KH. Nitrogen source-requirement and preference of ectomycorrhizal fungi in pure culture. Kor J Mycol 2013;41:149-59.   과학기술학회마을   DOI   ScienceOn
14 Harvey LM. Cultivation techniques for the production of ectomycorrhizal fungi. Biotech Adv 1991;9:13-29.   DOI   ScienceOn
15 Kasana RC, Salwan R, Dhar H., Dutt S, Gulati A. A rapid and easy method for the detection of microbial cellulases on agar plates using gram's iodine. Curr Microbiol 2008;57:503-7.   DOI   ScienceOn
16 Jeon SM, Kim MS, Ka KH. Effects of medium, temperature and pH on mycelial growth and cellulase activity of ectomycorrhizal fungi from Korean forests. Kor J Mycol 2012;40: 191-203.   과학기술학회마을   DOI
17 Ka KH, Park H, Hur TC, Bak WC. Selection of ectomycorrhizal isolates of Tricholoma matsutake and Tricholoma magnivelare for inoculation on seedlings of Pinus densiflora in vitro. Kor J Mycol 2008;36:148-52.   과학기술학회마을   DOI
18 Madigan MT, Martinko JM and Parker J. Brock biology of microorganisms. 9th ed. New Jersey: Prentice-Hall Inc.; 2000.
19 Read DJ, Leake JR, Langdale AR. The nitrogen nutrition of mycorrhizal fungi and their host plants. In: Nitrogen, phosphorus and sulphur utilization by fungi. Cambridge University Press; 1989. p. 181-204.
20 Kusuda M, Ueda M, Miyatake K, Terashita T. Characterization of the carbohydrase productions of an ectomycorrhizal fungus, Tricholoma matsutake. Mycoscience 2008;49:291-7.   DOI
21 Terashita T, Kono M, Yoshikawa K, Shishiyama J. Productivity of hydrolytic enzymes by mycorrhizal mushrooms. Mycoscience 1995;36:221-5.   DOI
22 Yamanaka T, Ota Y, Konno M, Kawai M, Ohta A, Neda H, Terashima Y, Yamada A. The host ranges of conifer-associated Tricholoma matsutake, Fagaceae-associated T. bakamatsutake and T. fulvocastaneum are wider in vitro than in nature. Mycologia 2014;106:397-406.   DOI
23 Choi HK. Nongzhenghuiao III. In: Lee GY, editor. Ancient agricultural books that were collected and translated by RDA (Rural Development Administration); 2007.