Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Evaluation of Cabbage- and Broccoli-genetic Resources for Resistance to Clubroot and Fusarium Wilt

뿌리혹병 및 시들음병에 대한 저항성 양배추와 브로콜리 유전자원 탐색

  • Lee, Ji Hyun (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Jo, Eun Ju (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Jang, Kyoung Soo (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Choi, Yong Ho (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Kim, Jin-Cheol (Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Choi, Gyung Ja (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
  • 이지현 (한국화학연구원 바이오화학연구센터) ;
  • 조은주 (한국화학연구원 바이오화학연구센터) ;
  • 장경수 (한국화학연구원 바이오화학연구센터) ;
  • 최용호 (한국화학연구원 바이오화학연구센터) ;
  • 김진철 (전남대학교 응용생물공학부) ;
  • 최경자 (한국화학연구원 바이오화학연구센터)
  • Received : 2014.10.21
  • Accepted : 2014.12.05
  • Published : 2014.12.31
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Abstract

Clubroot and Fusarium wilt of cole crops (Brassica oleracea L.) are destructive diseases which for many years has brought a decline in quality and large losses in yields all over the world. The breeding of resistant cultivars is an effective approach to reduce the use of chemical fungicides and minimize crop losses. This study was conducted to evaluate the resistance of 60 cabbage (B. oleracea var. capitata) and 6 broccoli (B. oleracea var. italica) lines provided by The RDA-Genebank Information Center to clubroot and Fusarium wilt. To investigate resistance to clubroot, seedlings of the genetic resources were inoculated with Plasmodiophora brassicae by drenching the roots with a mixed spore suspension (1 : 1) of two isolates. Of the tested genetic resources, four cabbage lines were moderately resistant and 'K166220' represented the highest resistance to P. brassicae. The others were susceptible to clubroot. On the other hand, to select resistant plants to Fusarium wilt, the genetic resources were inoculated with Fusarium oxysporum f. sp. conglutinans by dipping the roots in spore suspension of the fungus. Among them, 17 cabbage and 5 broccoli lines were resistant, 16 cabbage lines were moderately resistant, and the others were susceptible to Fusarium wilt. Especially, three cabbage ('IT227115', 'K161791', 'K173350') and two broccoli ('IT227100', 'IT227099') lines were highly resistant to the fungus. We suggest that the resistant genetic resources can be used as a basic material for resistant B. oleracea breeding system against clubroot and Fusarium wilt.

Brassica olerace의 주요 병해인 뿌리혹병과 시들음병에 대한 저항성 유전자원을 발굴하기 위하여, 농업유전자원정보센터로부터 제공받은 양배추(B. oleracea var capitata) 유전자원 60개와 브로콜리(B. oleracea var italica) 유전자원 6개의 뿌리혹병과 시들음병에 대한 저항성을 검정하였다. 유전자원의 뿌리혹병에 대한 저항성 검정을 위해서, 분양 받은 유전자원들의 유묘에 뿌리혹병균 Plasmodiophora brassicae의 포자현탁액을 접종하였다. 실험한 유전자원 중 양배추 유전자원 4개는 중도저항성을 보였으며, 이들 중 양배추 유전자원 'K166220'는 가장 높은 저항성을 나타냈다. 나머지 유전자원들은 감수성을 보였다. 한편, 유전자원의 시들음병에 대한 저항성을 검정하기 위하여 분양 받은 유전자원 유묘의 뿌리를 Fusarium oxysporum f. sp. conglutinans 포자 현탁액에 침지하여 접종하였다. 실험한 유전자원 중 양배추 유전자원 17개와 브로콜리 유전자원 5개는 저항성, 양배추 유전자원 16개는 중도저항성 그리고 나머지 유전자원은 감수성이었다. 특히 양배추 유전자원 3종('IT227115', 'K161791', 'K173350')과 브로콜리 유전자원 2종('IT227100', 'IT227099')은 시들음병에 대하여 높은 저항성을 보였다. 본 연구에서 확인된 뿌리혹병과 시들음병 저항성 유전자원들은 뿌리혹병과 시들음병 저항성 육종에 이용될 수 있을 것이다.

Keywords

References

  1. Arden, S. 1979. Fusarium yellows of cabbage and related crops. Vegetable Crops 730 pp.
  2. Arie, T., Kobayashi, Y., Okada, G., Kono, Y. and Yamaguchi, I. 1998. Control of soil borne clubroot disease of cruciferous plants by epoxydon from Phoma glomerata. Plant Pathol. 47: 743-748. https://doi.org/10.1046/j.1365-3059.1998.00298.x
  3. Armstrong, G. M. and Armstrong, J. K. 1952. Physiological races of the fusaria causing wilts of the Cruciferae. Phytopathology 42: 255-257.
  4. Armstrong, G. M. and Armstrong, J. K. 1966. Races of Fusarium oxysporum f. sp. conglutinans; race 4, new race; and a new host for race 1, Lychnis chalcedonica. Phytopathology 56: 525-530.
  5. Armstrong, G. M. and Armstrong, J. K. 1981. Formae speciales and races of Fusarium oxysporum causing wilt diseases. In: Fusarium Diseases, Biology and Taxonomy. eds. by P. E. Nelson, T. A. Toussoun and R. J. Cook. pp. 391-399. The Pennsylvania State University Press, University Park, USA.
  6. Baggett, J. R. and Kean, D. 1985. Clubroot-resistant broccoli breeding lines OSU CR-2 to OSU CR-8. Hort. Science 20: 784-785.
  7. Baik, S. Y., Kim, J.-C., Jang, K. S., Choi, Y. H. and Choi, G. J. 2011. Development of efficient screening method for resistance of cabbage to Fusarium oxysporum f. sp. conglutinans. Res. Plant Dis. 17: 13-18. (In Korean) https://doi.org/10.5423/RPD.2011.17.1.013
  8. Blank, L. M. 1937. Fusarium resistance in Wisconsin all seasons cabbage. J. Agric. Res. 55: 497-510.
  9. Bosland, P. W. and Williams, P. H. 1984. A new pathotype of the crucifer yellows organism capable of causing disease in cool soils. Cruciferae Newsletter 9: 95.
  10. Brayford, D. 1992. Fusarium oxysporum f. sp. conglutinans. IMI Descr. Fungi Bact. 1114: 1-2.
  11. Cartea, M. E., Velasco, P., Obregon, S., Padilla, G. and Haro, A. D. 2008. Seasonal variation in glucosinolate content in Brassica oleracea crops grown in Northwestern Spain. Phytochemistry 69: 403-410. https://doi.org/10.1016/j.phytochem.2007.08.014
  12. Crute, I. R., Gray, A. R., Crisp, P. and Buczacki, S. T. 1980. Variation in Plasmodiophora brassicae and resistance to clubroot disease in Brassicas and allied crops - a critical review. Plant Breed. Abs. 50: 91-104.
  13. Dobson, R. L., Gabrielson, R. L., Baker A. S. and Bennett, L. 1983. Effects of lime particle-size and distribution and fertilizer formulation on clubroot disease caused by Plasmodiophora brassicae. Plant Dis. 67: 50-52. https://doi.org/10.1094/PD-67-50
  14. Farnham, M. W., Keinath, A. P. and Smith, J. P. 2001. Characterization of Fusarium yellows resistance in collard. Plant Dis. 85: 890-894. https://doi.org/10.1094/PDIS.2001.85.8.890
  15. Figdore, S. S., Ferreira, M. E., Slocum, M. K. and Williams, P. H. 1993. Association of RFLP markers with trait loci affecting clubroot resistance and morphological characters in Brassica oleracea L. Euphytica 69: 33-44. https://doi.org/10.1007/BF00021723
  16. Grandclement, C. and Thomas, G. 1996. Detection and analysis of QTLs based on RADP markers for polygenic resistance to Plasmodiophora brassicae Woron. in Brassica oleracea L. Theor. Appl. Genet. 93: 86-90. https://doi.org/10.1007/BF00225731
  17. Hansen, M. 1989. Genetic variation and inheritance of tolerance to clubroot (Plasmodiophora brassicae Wor.) and other quantitative characters in cabbage (Brassica oleracea L.). Hereditas 110: 13-22.
  18. Hirai, M. 2006. Genetic analysis of clubroot resistance in Brassica crops. Breeding Sci. 56: 223-229. https://doi.org/10.1270/jsbbs.56.223
  19. James, R. V. and Williams, P. H. 1980. Clubroot resistance and linkage in Brassica campestris. Phytopathology 70: 776-779. https://doi.org/10.1094/Phyto-70-776
  20. Jo, S.-J., Jang, K. S., Choi, Y. H., Kim, J.-C. and Choi, G. J. 2010. Convenient screening method of Chinese cabbage for resistance to Plasmodiophora brassicae using soil-drenching inoculation. Res. Plant Dis. 16: 279-284. (In Korean) https://doi.org/10.5423/RPD.2010.16.3.279
  21. Jo, S.-J., Shim, S.-A., Jang, K. S., Choi, Y. H., Kim, J.-C. and Choi, G. J. 2011. Resistance of cultivars of Chinese cabbage to Plasmodiophora brassicae isolates of several races collected in Korea. Korean J. Hort. Sci. Technol. 29: 610-616. (In Korean)
  22. Jo, S.-J., Shim, S.-A., Jang, K. S., Choi, Y. H., Kim, J.-C. and Choi, G. J. 2012. Development of efficient screening method for resistant cabbage and broccoli to Plasmodiophora brassicae. Res. Plant Dis. 18: 86-92. (In Korean) https://doi.org/10.5423/RPD.2012.18.2.086
  23. Keinath, A. P., Farnham, M. W. and Smith, P. 1998. Reactions of 26 cultivars of Brassica oleracea to yellows in naturally infested soil. Biol. Cult. Tests 13: 155.
  24. Kim, C. H., Cho, W. D. and Lee, S. B. 2003. Review of researches on clubroot disease of Chinese cabbage in Korea and future tasks for its management. Res. Plant Dis. 9: 57-63. (In Korean) https://doi.org/10.5423/RPD.2003.9.2.057
  25. Korean Society of Plant Pathology (KSPP). 2009. Cabbage. In: List of Plant Disease in Korea. eds. by W.-G. Kim and H. M. Koo pp. 130-132. 5th ed. KSPP, Suwon, Korea.
  26. Kuginuki, Y., Yoshikawa, H. and Hirai, M. 1999. Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot resistance cultivars of Chinese cabbage (Brassica rapa L. ssp. pekinensis). Eur. J. Plant Pathol. 105: 327-332. https://doi.org/10.1023/A:1008705413127
  27. Landry, B. S., Hubert, N., Crete, R., Chiang, M. S., Lincoln, S. E. and Etoh, T. 1992. A genetic map for Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance gene to race 2 of Plasmodiophora brassicae (Woronin). Genome 35: 409-420. https://doi.org/10.1139/g92-061
  28. Laurence, F. and Thomas, G. 1993. Inheritance of resistance to clubroot (Plasmodiophora brassicae Wor.) in kale (Brassica oleracea ssp. acephala). Hereditas 119: 253-262.
  29. Lv, H., Yang, L., Kang, J., Wang, Q., Wang, X., Fang, Z., Liu, Y., Zhuang, M., Zhang, Y., Lin, Y., Yang, Y., Xie, B., Liu, B. and Liu, J.-S. 2013. Development of InDel markers linked to Fusarium wilt resistance in cabbage. Mol. Breeding 32: 961-967. https://doi.org/10.1007/s11032-013-9925-x
  30. Moon, Y. G., Kim, W. G., Cho, W. D. and Sung, J. M. 2001. Occurrence of Fusarium wilt on cruciferous vegetable crops and pathogenic differentiation of the causal fungus. Res. Plant Dis. 7: 93-101. (In Korean)
  31. Moriguchi, K., Kimizuka-Takagi, C., Ishii, K. and Nomura, K. 1999. A genetic map based on RAPD, RFLP, isozyme, morphological markers and QTL analysis for clubroot resistance in Brassica oleracea. Breeding Sci. 49: 257-265. https://doi.org/10.1270/jsbbs.49.257
  32. Niwa, R., Nomura, Y., Osaki, M. and Ezawa, T. 2008. Suppression of clubroot disease under neutral pH caused by inhibition of spore germination of Plasmodiophora brassicae in the rhizosphere. Plant Pathol. 57: 445-452. https://doi.org/10.1111/j.1365-3059.2007.01817.x
  33. Nomura, K., Minegishi, Y., Kimizuka-Takagi, C., Fujioka, T., Moriguchi, K., Shishido, R. and Ikehashi, H. 2005. Evaluation of F2 and F3 plants introgressed with QTLs for clubroot resistance in cabbage developed by using SCAR markers. Plant Breeding 124: 371-375. https://doi.org/10.1111/j.1439-0523.2005.01105.x
  34. Peterson, J. L. and Pound, C. S. 1960. Studies on resistance in radish to Fusarium oxysporum f. sp. conglutinans race 2. Phytopathology 50: 807-816.
  35. Ramirez-Villupadua, J., Endo, R. M., Bosland, P. and Williams, P. H. 1985. A new race of Fusarium oxysporum f. sp. conglutinans that attacks cabbage type A resistance. Plant Dis. 69: 612-613. https://doi.org/10.1094/PD-69-612
  36. Rocherieux, J., Glory, P., Giboulot, A., Boury, S., Barbeyron, G., Thomas, G. and Manzanares-Dauleux, M. J. 2004. Isolate-specific and broad-spectrum QTLs are involved in the control of clubroot in Brassica oleracea. Theor. Appl. Genet. 108: 1555-1563. https://doi.org/10.1007/s00122-003-1580-x
  37. Suwabe, K., Tsukada, H., Iketani, H., Hatakeyama, K., Fujimura, M., Nunome, T., Fukuoka, H., Matsumoto, S. and Hirai, M. 2003. Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L. Theor. Appl. Genet. 107: 997-1002. https://doi.org/10.1007/s00122-003-1309-x
  38. Voorrips, R. E. 1995. Plasmodiophora brassicae: aspects of pathogenesis and resistance in Brassica oleracea. Euphytica 83: 139-146. https://doi.org/10.1007/BF01678041
  39. Voorrips, R. E. and Visser, D. L. 1993. Examination of resistance to clubroot in accessions of Brassica oleracea L. using a glasshouse seedling test. Neth. J. Plant Pathol. 99: 269-276. https://doi.org/10.1007/BF01974308
  40. Voorrips, R. E., Jongerius, M. C. and Kanne, H. J. 1997. Mapping of two genes for resistance to clubroot (Plasmodiophora brassicae) in a population of doubled haploid lines of Brassica oleracea by means of RFLP and AFLP markers. Theor. Appl. Genet. 94: 75-82. https://doi.org/10.1007/s001220050384
  41. Walker, J. C. 1930. Inheritance of Fusarium resistance to cabbage yellows. J. Agric. Res. 40: 721-745.
  42. Walker, J. C. 1959. Progress and problems in controlling plant disease by host resistance. In: Plant Pathology Problems and Progress 1908-1958. eds. by C. S. Holton, G. W. Fischer, R. W. Fulton, H. Hart and S. E. A. MeCallan. pp. 32-41. Univ. Wisconsin Press, Madison.
  43. Yoon, C. S., Jung, E. K., Lee, S. J., Zhang, Y., Lee, J. E. and Kim, B. S. 2010. Screening of resistant Chinese cabbage cultivar against clubroot (Plasmodiophora brassicae) for cultivation in highland. Res. Plant Dis. 16: 9-65. (In Korean) https://doi.org/10.5423/RPD.2010.16.1.059
  44. Yoshikawa, H. 1993. Studies on breeding of clubroot resistance in cole (Cruciferae) crop. Bull. Natl. Res. Inst. Veg. Ornam. Plants Tea Jpn. Ser. A. 7: 1-165.
  45. Zhang, X. Z., Lee, S. U., Kim, J. S., Yoon, Y. S., Choi, G. S., Kim, H. K. and Kim, B. S. 2005. Control efficacy of flusulfamide GR on Chinese cabbage clubroot caused by Plasmodiophora brassicae. Res. Plant Dis. 11: 43-47. (In Korean) https://doi.org/10.5423/RPD.2005.11.1.043