Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Antioxidant Activity Analysis of Useful Compounds from Artemisiae Annuae Herba Using On-line Screening HPLC-ABTS+ Assay

On-line Screening HPLC-ABTS+ assay를 이용한 청호로부터 유용성분의 항산화 활성 분석

  • Lee, Kwang Jin (Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group) ;
  • Ma, Jin Yeul (Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group)
  • Received : 2014.03.17
  • Accepted : 2014.05.13
  • Published : 2014.12.31
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Abstract

The Antioxidant activity screening identification of five kind compounds in Artemisiae annuae herba with the on-line screening high performance liquid chromatography (HPLC) $ABTS^+$ assay. The various experimental variables such as the extraction time (h) and extraction solvent composition (%) of dipping method were investigated efficiently extraction at the room temperature $25^{\circ}C$. The results, the highest yield of total extract amount (0.458 g, 15.250%) was obtained by dipping method with 100% water and extraction time to 3 h. And the on-line screening HPLC-$ABTS^+$ assay method was rapid and efficient to search for bioactivity from natural products.

On-line screening high performance liquid chromatography (HPLC)-$ABTS^+$ assay를 이용한 청호로부터 chlorogenic acid, caffeic acid, vitexin, queretin, aremisinin의 항산화 활성을 온라인스크리닝 하였다. 이때 침적방법을 적용한 추출시간과 추출용매 조성으로부터 추출효율을 확인하였다. 이 결과 100% 물 추출물에서 수율이 가장 좋왔고 chloroenic acid의 항산화 활성이 가장 높았다. 또한 on-line screening HPLC-$ABTS^+$ assay 분석은 천연물에서 항산화 활성을 신속하게 탐색하는데 효율적이다.

Keywords

References

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