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http://dx.doi.org/10.3839/jabc.2014.047

Antioxidant Activity Analysis of Useful Compounds from Artemisiae Annuae Herba Using On-line Screening HPLC-ABTS+ Assay  

Lee, Kwang Jin (Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group)
Ma, Jin Yeul (Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group)
Publication Information
Journal of Applied Biological Chemistry / v.57, no.4, 2014 , pp. 301-305 More about this Journal
Abstract
The Antioxidant activity screening identification of five kind compounds in Artemisiae annuae herba with the on-line screening high performance liquid chromatography (HPLC) $ABTS^+$ assay. The various experimental variables such as the extraction time (h) and extraction solvent composition (%) of dipping method were investigated efficiently extraction at the room temperature $25^{\circ}C$. The results, the highest yield of total extract amount (0.458 g, 15.250%) was obtained by dipping method with 100% water and extraction time to 3 h. And the on-line screening HPLC-$ABTS^+$ assay method was rapid and efficient to search for bioactivity from natural products.
Keywords
analysis; annuae herba; artemisiae; identification; on-line screening high performance liquid chromatography;
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Times Cited By KSCI : 3  (Citation Analysis)
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