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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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The Extract from Lysimachia foenum-graecum Induces Apoptosis in MCF-7 Breast Cancer Cells

MCF-7 유방암 세포에서 영릉향 추출물에 의한 Apoptosis 유도에 관한 연구

  • Lee, Se Hee (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Guen Tae (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Jong Il (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Lim, Eun Gyeong (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, In Seop (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Young Min (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
  • 이세희 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 김근태 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 김종일 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 임은경 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 김인섭 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 김영민 (한남대학교 생명나노과학대학 생명시스템과학과)
  • Received : 2013.08.15
  • Accepted : 2013.10.21
  • Published : 2013.10.30
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Abstract

The extract from Lysimachia foenum-graecum (LFE) has been known to possess various instructive characters including anti-oxidant, anti-obesity, fungicidal activities. However, the accurate mechanism of those effects of LFE is not well known. In that respect, we evaluated the apoptotic effect and anti-cancer efficacy of extracts of LFE in MCF-7 breast cancer cells. In this study, we hypothesized that LFE may exert cancer cell apoptosis through regulating p53 and mitochondria-mediated apoptotic proteins. And this substance can generate ROS to cause free radical-induced apoptosis. Accordingly, the generation of ROS by LFE triggers the activation of p53 which are accompanied by pro-apoptotic protein activation and suppression of pro-survival proteins. We determined with MTT assay, flow cytometry for detection of intracellular ROS and Annexin V-PI staining, Western blotting. Consequently, our researches demonstrated that the treatment of LFE to breast cancer cells resulted in an activation of p53, Puma, Bax, cleaved-PARP and an inhibition of Bcl-2 expressions.

Keywords

References

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