DOI QR코드

DOI QR Code

이온성 액체를 사용한 꽃게 껍질에서 아스타크산틴 추출 조건의 최적화

Optimization of Extraction of Astaxanthin from Portunus trituberculatus by Ionic Liquids

  • Lee, Yu Jin (Department of Chemical Engineering, Inha University) ;
  • Lee, Yu Ri (Department of Chemical Engineering, Inha University) ;
  • Tang, Baokun (Department of Chemical Engineering, Inha University) ;
  • Row, Kyung Ho (Department of Chemical Engineering, Inha University)
  • 투고 : 2013.07.22
  • 심사 : 2013.08.28
  • 발행 : 2013.08.30

초록

Astaxanthin is one of the carotenoid with strong antioxidant. The conditions of extraction of astaxanthin from Portunus trituberculatus were optimized in this work. Six factors of conditions such as, extraction method, extraction solvent, ratio of solvent to raw material, temperature, and time, were investigated. For the increase of the extraction yield, ionic liquids were used as additives in the extraction solvent. The optimum extraction conditions were found: heat reflux extraction, Dichloromethane/methanol (25:75, v/v) as solvent, 1:30 of the ratio of solvent raw material, $80^{\circ}C$, 90 min, and ionic liquid as additive. As a result, 45.81 ${\mu}g/g$ of astaxanthin was extracted from waste.

키워드

참고문헌

  1. No, H. K. and M. Y. Lee (1995) Isolation of Chitin from crab shell weate. J. Korean Soc. Food Nutr. 24: 105-113.
  2. Yoon, C. H., H. S. Bok, D. K. Choi, and K. H. Row (2012) Optimization condition of astaxanthin extract from shrimp waste using response surface methodology. Korean Chem. Eng. Res. 50: 545-550. https://doi.org/10.9713/kcer.2012.50.3.545
  3. Kim, H. C., S. J. Park, J. K. Kim, Y. R. Kim, W. S. Park, Y. U. Cho, H. J. Cho, J. H. Kim, and Y. L. Ha (2001) Effect of the egg yolks from laying hens intubated astaxanthin on the macrophage activity, Hemagglutinintiter and Hemolysintiter. J. Korean Soc. Food Sci. Nutr. 30: 1283-1286.
  4. Kim, H. C., S. J. Park, J. K. Kim, C. W. Park, Y. U. Cho, H. J. Cho, and Y. L. Ha (2002) Effect of egg yolks from laying hens intubated astaxanthin on the oxidation of liver microsome of mouse. J. Korean Soc. Food Sci. Nutr. 31: 155-159. https://doi.org/10.3746/jkfn.2002.31.1.155
  5. Kim. H. and J. O. Kang (1998) Determination of astaxanthin, ${\alpha}$-tocopherol and tbars in the liver and muscle of rainbow trout supplemented with red yeast containing astaxanthin. J. Korean Soc. Food Sci. Nutr. 27: 935-939.
  6. R. Todd Lorenz and Gerald R. Cysewski (2000) Commercial potential for Haematococcus microalgae as a natural source of astaxanthin. Trends Biotechnol. 18: 160-167. https://doi.org/10.1016/S0167-7799(00)01433-5
  7. Lim, G. B., S. Y. Lee, E. K. Lee, S. J. Haam, and W. S. Kim (2002) Separation of astaxanthin from red yeast Phaffia rhodozyma by supercritical carbon dioxide extraction. J. Biochemical Eng. 11: 181-187. https://doi.org/10.1016/S1369-703X(02)00023-2
  8. Oh, S. Y., J. W. Kang, B. H. Park, and K. S. Kim (2012) Thermo-dynamic properties of imidazolium ionic liquids. Korean Chem. Eng. Res. 50: 708-712. https://doi.org/10.9713/kcer.2012.50.4.708
  9. R. Jakub., J. Vondrak, J. Michalek, Z. Micka (2006) Ternary polymer electrolytes with 1-methylimidazole based ionic liquids and aprotic solvents. Electrochimica Acta. 52: 1398-1408. https://doi.org/10.1016/j.electacta.2006.07.043
  10. Ngo, H. L., K. L. Compte, L. Hargens, and A. B. McEwen (2000) Thermal properties of imidazolium ionic liquids. Thermochim. Acta. 97: 357-361.
  11. Valenzuela, L. F., I. H. Ciaparai, and F. G. Valencia (2001) Supercritical cojethanol extraction of astaxanthin from blue crab (callinectes sapidus) shell waste. J. Food Process Eng. 24: 101-112. https://doi.org/10.1111/j.1745-4530.2001.tb00534.x
  12. Kim, S. Y., E. Cho, J. M. Yoo, M. J. In, and H. J. Chae (2008) Solubility and storage stability of astaxanthin. Korean J. Biotechnol. Bioeng. 23: 546-550.
  13. Chen, X., R. Chen, Z. Y. Guo, C. Li, and P. Li (2007) The preparation and stability of the inclusion complex of astaxanthin with ${\beta}$-cyclodextrin. Food Chem.101: 1580-1584. https://doi.org/10.1016/j.foodchem.2006.04.020
  14. Lee, S. K. and J. W. Kim (1994) Purification and characteization of a carotenoprotein from penaeus orientalis. J. Korean Chem. Soc. 38: 608-615.
  15. Bi, W., M. Tian, J. Zou, K. H. Row (2010) Task-specific ionic liquid-assisted extraction and separation of astaxanthin from shrimp waste. J. Chromatogr. B. 878: 2243-2248. https://doi.org/10.1016/j.jchromb.2010.06.034
  16. Ma, W. Y., Y. B. Lu, R. L. Hu, J. H. Chen, Z. H. Zhang, and Y. J. Pan (2010) Application of ionic liquids based microwave-assisted extraction of three alkaloids N-nornuciferine, O-nornuciferine, and nuciferine from lotus leaf. Talanta 80: 1292-1297. https://doi.org/10.1016/j.talanta.2009.09.027
  17. Lu, Y. B., W. Y. Ma, R. L. Hu, X. J. Dai, and Y. J. Pan (2008) Ionic liquid-based microwave-assisted extraction of phenolic alkaloids from the medicinal plant Nelumbo nucifera Gaertn. J. Chromatogr. A. 1208: 42-46. https://doi.org/10.1016/j.chroma.2008.08.070
  18. Rostagno, M. A., M. Palma, and C. G. Barroso (2007) Microwave assisted extraction of soy isoflavones. Anal. Chim. Acta. 588: 274-282. https://doi.org/10.1016/j.aca.2007.02.010
  19. Sachindra, N. M., N. Bhaskar, and N. S. Mahendrakar (2005) Carotenoids in crabs from marine and fresh waters of India. Lebens Wiss und-Technol. 38: 221-225. https://doi.org/10.1016/j.lwt.2004.06.003

피인용 문헌

  1. Ionic-Liquid-Mediated Extraction and Separation Processes for Bioactive Compounds: Past, Present, and Future Trends vol.117, pp.10, 2017, https://doi.org/10.1021/acs.chemrev.6b00550