• Animal cells and systems
• /
• 제13권3호
• /
• pp.305-314
• /
• 2009

The control region of mitochondrial DNA (13516-14619) is located between srRNA and $tRNA^{lle}$ gene in swimming crab, Portunus trituberculatus. The present study was investigated the genetic polymorph isms of the control region in samples of P. trituberculatus collected at coastal waters of the Yellow Sea in Korea. A total of 300 substitution and indel polymorphic sites were identified. In addition to SNPs and indel variation, a hypervariable microsatellite motif was also identified at position from 14358 to 14391, which exhibited 10 alleles including 53 different suballeles. When the hypervariable microsatellite motif was removed from the alignment, 95 haplotypes were identified (93 unique haplotypes). The nucleotide and haplotype diversities were ranged from 0.024 to 0.028 and from 0.952 to 1.000, respectively. The statistically significant evidence for geographical structure was not detected from the analyses of neighbor-joining tree and minimum-spanning network, neither. This result suggest that population of P. trituberculatus are capable of extensive gene flow among populations. We believed that the polymorph isms of the control region will be used for informative markers to study phylogenetic relationships of P. trituberculatus.

• Ocean and Polar Research
• /
• 제39권3호
• /
• pp.213-219
• /
• 2017

In this study, we analyzed the economic feasibility of the swimming crab (Portunus trituberculatus) fry releasing program in the West Sea of Korea. The catch rate of released fry in 2010, measured by genetic markers, was applied to the economic surplus method to estimate benefits. As a result of our analysis, the B/C ratio, as an indicator of economic evaluation, was determined to be 2.168, which means that the releasing program was economically feasible. And it was shown that the benefits to consumers is six times greater than the benefits to producers, confirming the necessity of the releasing program as a public work.

• KSBB Journal
• /
• 제28권4호
• /
• pp.238-243
• /
• 2013

Astaxanthin is one of the carotenoid with strong antioxidant. The conditions of extraction of astaxanthin from Portunus trituberculatus were optimized in this work. Six factors of conditions such as, extraction method, extraction solvent, ratio of solvent to raw material, temperature, and time, were investigated. For the increase of the extraction yield, ionic liquids were used as additives in the extraction solvent. The optimum extraction conditions were found: heat reflux extraction, Dichloromethane/methanol (25:75, v/v) as solvent, 1:30 of the ratio of solvent raw material, $80^{\circ}C$, 90 min, and ionic liquid as additive. As a result, 45.81 ${\mu}g/g$ of astaxanthin was extracted from waste.

• 생명과학회지
• /
• 제23권9호
• /
• pp.1088-1095
• /
• 2013

꽃게(Portunus trituberculatus)는 세계적으로 넓게 분포하는 갑각류로 모래나 돌멩이가 있는 해저에 서식한다. 본 연구는 서해의 4개 지점(영광, 태안, 소래, 연평도)에서 채집된 P. trituberculatus 281 개체에 대해 10 종류 microsatellite 좌위의 유전적 다형성을 조사하였다. 좌위당 대립유전자 수는 50-129개로 평균 69.5개였으며, 관측 및 예상 이형접합도는 각각 0.111-1.000 및 0.609-0.979 범위에 있었다. 좌위별 근친계수((Fis)는 -0.0207에서 0.8175 범위였다. 유전적 분화도(Fst)는 0.05보다 낮게 나타났는데, 이것은 4 꽃게 간의 유전적 분화(genetic differentiation)가 매우 낮은 이루어진 것으로 추정하게 한다. UPGMA을 이용한 계통도 작성에서도 4 그룹 간의 유전적 거리는 매우 가깝다는 결과를 얻을 수 있었다. 매우 높은 다형성과 집단간의 낮은 유전적 분화는 서해안의 꽃게 집단은 활발한 유전적 흐름(gene flow)이 일어나며, 그룹간 지리적 경계가 없음을 제시한다

• 한국어병학회지
• /
• 제10권1호
• /
• pp.65-72
• /
• 1997

유용 갑각류인 꽃게, 중하 및 징거미새우의 유생과 성체에 대한 황화수소의 급성독성 실험 결과로부터 이들 갑각류에 대한 독성수준, 독성순위 및 유생과 성체와의 독성차를 검토하였다. 황화수소에 대한 꽃게, 중하 및 징거미새우 유생의 96시간 반수치사농도(96hr-$LC_{50}$)는 각각 7.3, 9.3 및 $9.0{\mu}g/\ell$으로 독성순위는 꽃게>징거미새우>중하순으로 나타났다. 한편, 성체에 대한 황화수소의 96hr-$LC_{50}$값은 꽃게 $42.5{\mu}gS/\ell$, 중하 $37.8{\mu}gS/\ell$, 징거미새우 $56.6{\mu}gS/\ell$로 독성순위는 중하>꽃게>징거미새우 순으로 유생과는 다른 경향을 나타내었다. 황화 수소는 꽃게, 중하 및 징거미새우의 유생에 대하여 성체에 비해 각각 5.8, 4.1, 6.3배의 강한 독성을 나타냈다.

• 한국수산과학회지
• /
• 제42권1호
• /
• pp.48-55
• /
• 2009

The gonad maturation and spawning of blue crab, Portunus trituberculatus (Miers, 1876) using samples caught by trammer net in the western coast of Korea from January to December. The gonadosomatic index (GSI) showed the highest value of 15.0 in June and lowest value of 1.29 in August. Hepatosomatic index (HSI) showed the lowest in the spawning season from June to July. Thereafter, from August the value started to increase to 5.77 in December, showing an adverse trend against GSI. In the histological studies of the female reproductive organ, the gonad filled with primary oocytes from January to February and secondary oocytes in May. The female gonads mature from June, and mature and immature groups could be distinguished from December to February or March. In August, female developed another cycle of gonad development, showing new primary oocytes in the gonads again. Some of female crabs had receptaculum filled with already active sperm cells in it from March to May. In the time of August to September, almost all the females had the sperm sacs in the receptaculum. From the result, the coupling period estimated from August to September. Total RNA and DNA values of the crabs were highest in May (2.51, $0.57{\mu}g/mg$). The DNA value remained unchanged except for the value in May ($0.09-0.13{\mu}g/mg$). while RNA value was lowest in January ($1.08{\mu}g/mg$). The RAN/DNA ratio was the lowest (6.23%) in May and highest (18.05%) in July. The value of correlations coefficient between body weight and fecundity of the crabs was higher that of between carapace width and fecundity.

• 한국수산과학회지
• /
• 제7권1호
• /
• pp.28-36
• /
• 1974

꽂게 (Portunus trituberculatus) 240미, 민꽂게(Charybdis japonica) 78미 등을 2월 21일 부터 8월 7일 까지 사육하면서, 이들이 먹이를 먹을 때 내는 소리를 녹음실의 간이 수조속에서 녹음하고, 행동을 관찰하였으며, 녹음된 소리를 증폭시켜 무향 수조 속에서 수중 스피커로 방성하였을 때, 수조속 꽃게들의 반응결과와 녹음된 소리를 분석한 결과를 요약하면 다음과 같다. 1. 꽃게들이 내는 소리의 음압은 크기별, 군별로 다르며, $52\~124db$이고, 주파수는 $250\~1000Hz$이다. 2. 꽃게의 내는 소리를 방성할때 추음효과는 약 $70\%$이다. 3. 꽃게의 추음속도는 $3\~6cm/sec$이며, 스피커에 도달한 게는 그곳을 떠나지 않는다. 4, 꽃게의 소리를 이용하여 음향어법에 적용할 때 꽃게의 크기는 갑장 $5.6\~6.5Cm$, 갑폭 $12.6\~15cm$인 암컷이 적당하다.

• 수산해양교육연구
• /
• 제25권4호
• /
• pp.991-997
• /
• 2013

Distribution and occurrence of swimming crab, Portunus trituberculatus larvae were investigated in the mid-western coast of Korea in the Yellow Sea. P. trituberculatus larvae were collected in July and August from 2010 to 2012. Bongo net with 303 mesh was deployed once with a double oblique tow. Zoea I (ZI) densities were highest in all sampling months. Then densities of later larval stages decreased dramatically. In general, larval densities at the stations in northern parts and coastal areas were higher than those at the southern and offshore area. Because egg bearing seasons of P. trituberculatus in the study area are between April and August, larval densities, particularly, of ZI may be underestimated. Considering higher densities of ZI and lower ones of later stages, larvae may be transported to growing area and returned to the parental populations. Larval densities and sea surface temperature were not correlated.

• 한국발생생물학회지:발생과생식
• /
• 제25권3호
• /
• pp.185-192
• /
• 2021

The five oligonucleotide primers (oligo-primers) turned out a total of 335 fragments (FMs) (52.9%) in the blue crab (Portunus trituberculatus) group alpha and 298 FMs (47.1%) in the crab group beta, with the FM scales range varying from 100 bp to 2,000 bp. The highest band-sharing (BS) value (0.907) was found between individual's no. 19 and no. 20 within the blue crab group beta. Parties in the blue crab group beta (0.601±0.017) had higher BS rates than did parties from the crab group alpha (0.563±0.017) (p<0.05). The polar dendrogram got by the five oligo-primers points out two genetic extents: bundle I (BLUECRAB 01, 03, 04, 05, 06, 08, and 10) and bundle II (BLUECRAB 02, 07, 09. 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, and 22). The OPD-01 primer revealed 22 loci shared by all the examples of the as FMs of 1,000 bp. The oligo-primer OPA-05 made unique loci shared to each group (ULSEG), almost 400 bp and 500 bp, individually, in blue crab group beta. The remaining oligo-primers did not reveal any loci shared by the two crab groups (LSTG). The average number of ULSEG was diverse and 1.6-fold higher in the crab group beta than in the crab group alpha.

• 수산해양교육연구
• /
• 제29권2호
• /
• pp.422-435
• /
• 2017

Intermolt periods, growth rates, survival (%) and relative growth of the megalopa larvae of Portunus trituberculatus (Miers, 1876) were studied up to the crab 7th stage for 160 days in the 3 different temperature groups in which each has 60 larvae. The higher the water temperature was the shorter the intermolt period was in each crab stage. In addition, a deviation of intermolt periods was shown as few as the water temperature gets higher. The intermolt period in the 7th crab stage was $29.8{\pm}3.26$ days in the experimental group at the room temperature, $45.2{\pm}3.89$ days at the temperature of $17^{\circ}C$, and $25.6{\pm}2.23$ days at the temperature of $27^{\circ}C$, respectively. The survival (%) of larvae of P. trituberculatus (the crab 7th stage) is the highest in the group at the room temperature: However, they showed 15% at the temperature of $27^{\circ}C$ and 10% at the temperature of $17^{\circ}C$. All the groups were shown the similar relative growth, but significant differences appeared in some comparison. The sizes (mean growth of carapace width) of the crabs in the group at the temperature of $27^{\circ}C$ reached 5.01~25.45 mm length (it is the longest among the groups) from the crab 1th to the crab 7th stage. However, the sizes (mean growth of carapace width) of room temperature were 4.99~22.26 mm and the size of crab in the group at the temperature of $17^{\circ}C$ reached 4.8~17.26 mm, The sizes (mean growth of carapace length) of the crabs in the group at temperature of $27^{\circ}C$ reached 2.88~13.56 mm, However, the size of the crab in the group at the room temperature showed 2.88~12.34 mm, while in the group at temperature of $17^{\circ}C$, their average growth of carapace length were 2.51~8.03 mm. The growth rates of larvae of P. trituberculatus (from the crab 1th to the crab 7th stage) were changed with the increase of the instar stage, however their changes showed irregular.