Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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A Novel Microcystin-degrading Bacterium, Microbacterium sp. MA21

Microcystin을 분해하는 신균주 Microbacterium sp. MA21

  • Ko, So-Ra (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Young-Ki (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh, Hee-Mock (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn, Chi-Yong (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • 고소라 (한국생명공학연구원 환경바이오연구센터) ;
  • 이영기 (한국생명공학연구원 환경바이오연구센터) ;
  • 오희목 (한국생명공학연구원 환경바이오연구센터) ;
  • 안치용 (한국생명공학연구원 환경바이오연구센터)
  • Received : 2012.09.27
  • Accepted : 2013.06.03
  • Published : 2013.06.30
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Abstract

A microcystin-degrading bacterium was isolated from Daechung reservoir, Korea. The isolated bacterium was identified as Microbacterium sp. by 16S rRNA gene sequence analysis, and designated as Microbacterium sp. MA21. This strain degraded cyanobacterial hepatotoxin, microcystin-LR, over 80% when incubated at $30^{\circ}C$ for 12 hr in R2A medium. Two unknown metabolites of microcystin were also identified during the degradation process. Although only Sphinogomonas and Actinobacteria have been known to degrade microcystin previously, this is the first report that Microbacterium sp. MA21 could degrade microcystin.

환경시료에서 마이크로시스틴 분해능을 나타낸 균주 1종을 분리하였고 16S rRNA gene sequence 분석 결과, Microbacterium sp.로 동정되어 Microbacterium sp. MA21로 명명하였다. R2A배지를 기본 배지로 하여 $50{\mu}g\;L^{-1}$ microcystin-LR을 첨가하여 $30^{\circ}C$, 12시간 동안 배양한 후 PPIA를 통해 microcystin이 80% 이상 분해되는 것을 확인하였다. Microcystin-LR의 분해를 HPLC 분석을 통해 재확인하였고, microcystin 분해산물로 추정되는 두 개의 peak를 확인하였다. 16S rRNA 염기서열을 이용한 계통분류 분석 결과, 본 연구에서 분리한 Microbacterium sp. MA21은 Alphaproteobacteria의 Sphingomonas 속에 속하지 않는 것은 물론 Actinobacteria에는 속하지만 기존에 보고되지 않은, 새로운 genus로 확인되었다.

Keywords

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