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http://dx.doi.org/10.11626/KJEB.2013.31.2.158

A Novel Microcystin-degrading Bacterium, Microbacterium sp. MA21  

Ko, So-Ra (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Lee, Young-Ki (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Oh, Hee-Mock (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Ahn, Chi-Yong (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Korean Journal of Environmental Biology / v.31, no.2, 2013 , pp. 158-164 More about this Journal
Abstract
A microcystin-degrading bacterium was isolated from Daechung reservoir, Korea. The isolated bacterium was identified as Microbacterium sp. by 16S rRNA gene sequence analysis, and designated as Microbacterium sp. MA21. This strain degraded cyanobacterial hepatotoxin, microcystin-LR, over 80% when incubated at $30^{\circ}C$ for 12 hr in R2A medium. Two unknown metabolites of microcystin were also identified during the degradation process. Although only Sphinogomonas and Actinobacteria have been known to degrade microcystin previously, this is the first report that Microbacterium sp. MA21 could degrade microcystin.
Keywords
degrading bacteria; HPLC; microcystin-LR; Microbacterium sp.;
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1 Okano K, K Shimizu, Y Kawauchi, H Maseda, M Utsumi, Z Zhang, BA Neilen and N Sugiura. 2009. Characteristics of a microcystin-degrading bacterium under alkaline environmental conditions. J. Toxicol. 2009:954291.
2 Maruyama T, K Kato, A Yokoyama. 2003. Dynamics of microcystin- degrading bacteria in mucilage of Microcystis. Microb. Ecol. 46:279-288.   DOI
3 National Institute of Environmental Research. 2008. Study on the alert criteria of harmful algal bloom alert system (I). NIER No. 2008-49-999.
4 Oh HM, SJ Lee, JH Kim, HS Kim and BD Yoon. 2001. Seasonal variation and indirect monitoring of microcystin concentration in Daechung Reservoir, Korea. Appl. Environ. Microbiol. 67:1484-1489.   DOI
5 Sivonen K and G Jones. 1999.Cyanobacterial toxins. pp.41-111 In Toxic cyanobacteria in water: a guide to their public health consequences, monitoring, and management (Chorus I and J Bartram eds.). E&FN Spon. London.
6 Park HD, Y Sasski, T Maruyama, E Yanagisawa, A Hiraishi and K Kato. 2001. Degradation of the cyanobacterial hepatotoxin microcystin by a new bacterium isolated from a hypertrophic lake. Environ. Toxicol. 16:337-343.   DOI
7 Rapala J, KA Berg, C Lyra, RM Niemi, W Manz, S Suomalainen, L Paulin and K Lahti. 2005. Paucibacter toxinivorans gen. nov., sp. nov., a bacterium that degrades cyclic cyanobacterial hepatotoxins microcystins and nodularin. Int. J. Syst. Evol. Microbiol. 55:1563-1568.   DOI
8 Shimizu K, H Maseda, K Okano, T Itayama, Y Kawauchi, R Chen, M Utsumi, Z Zhang and N Sugiura. 2011. How microcystin- degrading bacteria express microcystin degradation activity. Lakes Reserv.: Res. Manage. 16:169-178.   DOI
9 Valeria AM, EJ Ricardo and WD Alberto. 2006. Degradation of microcystin-RR by a new isolated Sphingomonas sp. CBA4 isolated from San Roque reservoir (Córdoba-Argentina). Biodegradation 17:447-455.   DOI
10 Wang JF, PF Wu, J Chen and H Yan. 2010. Biodegradation of microcystin-RR by a new isolated Sphingopyxis sp. USTB-05. Chin. J. Chem. Eng. 18:108-112.   DOI
11 WHO. 1998. Guidelines for drinking-water quality. pp.95-110. In Health Criteria and Other Supporting Information, second ed. World Health Organization, Geneva.
12 Yan H, G Pan, H Zou, XL Li and H Chen. 2004. Effective removal of microcystins using carbon nanotubes embedded with bacteria. Chin. Sci. Bull. 49:1694-1698.   DOI
13 Yoon KH. 2011. Production and properties of hemicelluloses by an isolate of Microbacterium sp. Kor. J. Microbiol. 47: 225-230.
14 Zhou L, L Hou, Y Hu, J Song and W Chen. 2010. Effects of wattle extract in Microcystis aeruginosa growth and the simulated mini fresh water ecosystem. J. Environ. Biol. 31:1023-1030.
15 Hummert C, J Dahlmann, M Reichelt and B Luckas. 2001. Analytical techniques for monitoring harmful cyanobacteria in lakes. Lakes Reserv.: Res. Manage. 44:159-168.
16 Bourne DG, P Riddles, GJ Jones, W Smith and RL Blakeley. 2001. Characterization of a gene cluster involved in bacterial degradation of the cyanobacterial toxin microcystin LR. Environ. Toxicol. 16:523-534.   DOI
17 Ho L, D Hoefel, CP Saint and G Newcombe. 2007. Isolation and identification of a navel microcystin-degrading bacterium from a biological sand filter. Water Res. 41:4685-4695.   DOI
18 Hu LB, JD Yang, W Zhou, YF Yin, J Chen and ZQ Shi. 2009. Isolation of a Methylobacillus sp. that degrades microcystin toxins associated with cyanobacteria. New Biotechnol. 26: 205-211.   DOI
19 Ishii H, M Nishijima and T Abe. 2004. Characterization of degradation process of cyanobacterial hepatotoxins by a gram-negative aerobic bacterium. Water Res. 38:2667- 2676.   DOI
20 Jones GJ and PT Orr. 1994. Release and degradation of microcystins following algicide treatment of a Microcystis aeruginosa bloom in a recreational lake, as determined by HPLC and protein phosphatase inhibition assay. Water Res. 28: 871-876.   DOI
21 Lane DJ. 1991. 16S/23S rRNA sequencing. pp.115-175. In Nucleic acid techniques in bacterial systematics (Stackebrandt E and M Goodfellow eds.). Wiley. New York.
22 Kim DJ, SH Hong and TS Ahn. 1999. Seasonal and vertical change of bacterial communities in Lake Soyang. Kor. J. Microbiol. 35:242-247.
23 Kim HB, HK Park and JS Moon. 2009. Evaluation of pre-treatment stages for microcystins analysis using LC/MS/MS. J. Kor. Soc. Water Qual. 25:720-726.
24 Kim SG, SK Rhee, CY Ahn, SR Ko, GG Choi, JW Bae, YH Park and HM Oh. 2006. Determination of cyanobacterial diversity during algal blooms in Daechung Reservoir, Korea, on the basis of cpcBA intergenic spacer region analysis. Appl. Environ. Microbiol. 72:3252-3258.   DOI
25 Lee HK, JH Kim, SA Yoo, TS Ahn, CK Kim and DH Lee. 2003. Primer evaluation for the detection of toxigenic Microcystis by PCR. Kor. J. Microbiol. 39:166-174.
26 Bourne DG, GJ Jones, L Blakeley, A Jones, AP Negri and P Riddles. 1996. Enzymatic pathway for the bacterial degradation of the cyanobacterial cyclic peptide toxin microcystin- LR. Appl. Environ. Microbiol. 62:4086-4096.
27 Lemes GAF, R Kersanach, LS Pinto, OA Dellagostin, JS Yunes and A Matthiensen. 2008. Biodegradation of microcystins by aquatic Burkholderia sp. from a South Brazilian coastal lagoon. Ecotoxicol. Environ. Saf. 69:358-365.   DOI
28 Manage PM, C Edwards, BK Singh and LA Lawton. 2009. Isolation and identification of novel microcystin-degrading bacteria. Appl. Environ. Microbiol. 75:6924-6928.   DOI
29 An J and WW Carmichael. 1994. Use of a colorimetric protein phosphatase inhibition assay and enzyme linked immunosorbent assay for the study of microcystins and nodularins. Toxicon. 32:1495-1507.   DOI