Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biocontrol of Late Blight and Plant Growth Promotion in Tomato Using Rhizobacterial Isolates

  • Lamsal, Kabir (Department of Applied Plant Sciences, Kangwon National University) ;
  • Kim, Sang Woo (Department of Applied Plant Sciences, Kangwon National University) ;
  • Kim, Yun Seok (Department of Applied Plant Sciences, Kangwon National University) ;
  • Lee, Youn Su (Department of Applied Plant Sciences, Kangwon National University)
  • Received : 2012.09.27
  • Accepted : 2013.03.16
  • Published : 2013.07.28
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Abstract

Seven bacterial isolates (viz., AB05, AB10, AB11, AB12, AB14, AB15, and AB17) were derived from the rhizosphere and evaluated in terms of plant growth-promoting activities and the inhibition of Phytophthora infestans affecting tomatoes in Korea. According to 16S rDNA sequencing, a majority of the isolates are members of Bacillus, and a single isolate belongs to Paenibacillus. All seven isolates inhibited P. infestans by more than 60% in vitro. However, AB15 was the most effective, inhibiting mycelial growth of the pathogen by more than 80% in vitro and suppressing disease by 74% compared with control plants under greenhouse conditions. In a PGPR assay, all of the bacterial isolates were capable of enhancing different growth parameters (shoot/root length, fresh biomass, dry matter, and chlorophyll content) in comparison with non-inoculated control plants. AB17-treated plants in particular showed the highest enhancement in fresh biomass with 18% and 26% increments in the root and shoot biomass, respectively. However, isolate AB10 showed the highest shoot and root growth with 18% and 26% increments, respectively. Moreover, the total chlorophyll content was 14%~19% higher in treated plants.

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References

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