Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A Novel Marker for the Species-Specific Detection and Quantitation of Vibrio cholerae by Targeting an Outer Membrane Lipoprotein lolB Gene

  • Cho, Min Seok (National Academy of Agricultural Science, Rural Development Administration) ;
  • Ahn, Tae-Young (Department of Microbiology, Dankook University) ;
  • Joh, Kiseong (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Paik, Soon-Young (Department of Microbiology, College of Medicine, The Catholic University of Korea) ;
  • Kwon, Oh-Sang (Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research Environmental Research Complex) ;
  • Jheong, Won-Hwa (Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research Environmental Research Complex) ;
  • Joung, Yochan (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Park, Dong Suk (National Academy of Agricultural Science, Rural Development Administration)
  • Received : 2012.08.14
  • Accepted : 2012.09.28
  • Published : 2013.04.28
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Abstract

Vibrio cholerae O1 and O139 are the major serotypes associated with illness, and some V. cholera non-O1 and non-O139 isolates produce cholera toxin. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the species-specific detection and quantitation of V. cholera using a primer pair based on an outer membrane lipoprotein lolB gene for the amplification of a 195 bp DNA fragment. The qPCR primer set for the accurate diagnosis of V. cholera was developed from publically available genome sequences. This quantitative PCR-based method will potentially simplify and facilitate the diagnosis of this pathogen and guide disease management.

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References

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