KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Screening of Peptide Sequences Cognitive of Pb2+ by Biopanning

바이오패닝에 의한 Pb2+ 친화성 펩타이드 서열의 탐색

  • Nguyen, Thuong T.L. (School of Chemical Engineering and Bioengineering, University of Ulsan) ;
  • Hong, Soon Ho (School of Chemical Engineering and Bioengineering, University of Ulsan) ;
  • Choe, Woo-Seok (School of Chemical Engineering, Sungkyunkwan University) ;
  • Yoo, Ik-Keun (School of Chemical Engineering and Bioengineering, University of Ulsan)
  • ;
  • 홍순호 (울산대학교 화학공학부) ;
  • 최우석 (성균관대학교 화학공학부) ;
  • 유익근 (울산대학교 화학공학부)
  • Received : 2013.01.31
  • Accepted : 2013.06.25
  • Published : 2013.06.27
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Abstract

For the selection of peptide specifically binding to $Pb^{2+}$, the biopanning with the commercially available Ph.D.-7 phage displayed heptapeptide library was carried out against $Pb^{2+}$ immobilized on a metal-chelating IDA (iminodiacetic acid) resin. After four rounds of screening against $Pb^{2+}$-IDA including negative selections against charged bead with metal ions other than $Pb^{2+}$ and uncharged bead, several candidate lead-binding phage peptides were initially determined based on the order of frequency from the screened phage clones. Of the selected phage peptide sequences, the peptide of the highest frequency, CysSerIleArgThrLeuHisGlnCys (CSIRTLHQC) also exhibited the strongest affinity for $Pb^{2+}$ in binding assays for individual phage clones. However, there was not a significant difference in $Pb^{2+}$ affinity between selected peptides when using synthetic heptapeptides corresponding to the displayed peptide sequences of phage clones.

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References

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