KSBB Journal

  • 제28권2호
  • /
  • Pages.74-79
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  • 2013
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  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
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지칭개에서 분리한 Hemistepsin A와 B의 비듬균에 대한 항균효과

Antifungal Activities of Hemistepsin A and B Isolated from Hemistepita lyrata Bunge against Dandruff-causing Microbe Malassezia obutusa

  • 이종록 (대구한의대학교 제약공학과) ;
  • 정대화 (대구한의대학교 한방생명자원연구센터) ;
  • 박문기 (대구한의대학교 제약공학과)
  • Lee, Jong Rok (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Jung, Dae Hwa (Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Park, Moon Ki (Department of Pharmaceutical Engineering, Daegu Haany University)
  • 투고 : 2012.11.07
  • 심사 : 2013.01.24
  • 발행 : 2013.04.27
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초록

There are a large number of bioactive sesquiterpene lactones from compositae plants including Hemistepita lyrata Bunge. In the present study, we purified two sesquiterpene lactones, Hemistepsin A and B, from H. lyrata and evaluated their antimicrobial activities against Malassezia obutusa. Chromatographic separation was used for the preparation of Hemistepsin A and B, and the identity of these compounds was confirmed by NMR. Strong inhibition of growth of M. obutusa was obtained with all doses of Hemistepsin A tested. Moreover, antifungal activity of Hemistepsin A occurred in a dose-dependent manner. Hemistepsin B also showed potent antifungal activity at the dose of $800{\mu}m/disc$. From these results, it was suggested that Hemistepsin A and B be beneficial for the preparation of the useful agent for treating scalp diseases occurred by dandruff-causing Malassezia species.

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참고문헌

  1. Peoples Press (1977) In Encyclopedia of the Traditional Chinese Materia Medica. pp. 1458-1459. Peoples Press, Shanghai, China.
  2. Jang, D. S., M. S. Yang, and K. H. Park (1998) Sesquiterpene Lactone from Hemisteptia lyrata. Planta Med. 64: 289-290. https://doi.org/10.1055/s-2006-957436
  3. Jang, D. S., T. J. Ha, M. S. Yang, and K. H. Park (1999) Hemistepsins with Cytotoxic Activity from Hemisteptia lyrata. Planta Med. 65: 765-766. https://doi.org/10.1055/s-2006-960863
  4. Ha, T. J., J. R. Lee, J. Lee, K. H. Park, and M. S. Yang (2001) Studies on the Constituents from the Flowers of Hemisteptia lyrata (Bunge) (I). Kor. J. Pharmacogn. 32: 238-241.
  5. Ha, T. J., D. S. Jang, K. D. Lee, J. R. Lee, K. H. Park, and M. S Yang (2002) Studies on the Constituents from the Flowers of Hemisteptia lyrata (Bunge) (II). Kor. J. Pharmacogn. 33: 92-95.
  6. Jang, D. S., K. H. Park, H. M. Kim, D. H. Hong, H. K. Chun, Y. H. Kho, and M. S. Yang (1998) Biological Activities of Sesqurterpene Lactones Isolated from Several Compositae Plants. Part 1 Cytotoxicity Against Cancer Cell Lines. Kor. J. Pharmacogn. 29: 243-247.
  7. Jang, D. S., B. M. Kwon, and M. S. Yang (1999) Biological Activites of Sesquiterpene Lactones Isolated from Several Compositae Plants, Part 2 FPTase Inhibitory Activity. Kor. J. Pharmacogn. 30: 70-73.
  8. Jang, D. S., K. H. Park, H. L. Ko, H. S. Lee, B. M. Kwon, and M. S. Yang (1999) Biological Activities of Sesquiterpene Lactones Isolated from Several Compositae Plants. Part 3 Inhibitory Activity on Nitric Oxide Release and ACAT. Kor. J. Pharmacogn. 30: 74-78.
  9. McGinley, K. J., J. J. Leyden, and R. R. Marples (1979) Quantitative microbiology of the scalp in non-dandruff, dandruff and seborrheic dermatitis. J. Invest. Dermatol. 64: 401-405.
  10. Roberts, S. B. (1969) Pityrosporum orbiculare incidence and disribution in clinically normal skin. Br. J. Dermatol. 81: 264-269. https://doi.org/10.1111/j.1365-2133.1969.tb13978.x
  11. Choi, I. (2003) Antimicrobial activity of Rhus javanica extracts against animal husbandry disease-related bacteria. J. Kor. Soc. Food Sci. Nutr. 32: 1214-1220. https://doi.org/10.3746/jkfn.2003.32.8.1214
  12. Hyeon, J. J., D. W. Kim, J. B. Jun, and S. L. Chung (1993) Statistical and mycologic studies on dandruff. Kor. J. Dermatol. 31: 164-174.
  13. Lee, S. H. and J. S. Lee (2007) Production and Characteristics of Antidandruffy Compound from Chrysanthemum zawadskii. Kor. J. Microbiol. Biotechnol. 35: 220-225.
  14. Lee, S. K. (2003) Antimicrobial effecdt of Bamboo (Phyllosrachys Bambusoides) Essential oil on Trichophyton and Pityrosporum. J. Fd Hyg. Safety. 18: 113-117.
  15. Ryu, M. J., Y. S. Rim, D. I. Choi, and S. Y. Lee (2011) Anti-fungal Activity against Malassezia furfur and Anti-inflammatory Activity in RAW 264.7 Cells of the Sanguisorba officinalis Extracts. Kor. J. Society of Cosmetology. 17: 225-232.
  16. Suk, K. D. (2004) Inhibitory effect of Resina Pini on growth of Malassezia furfur Bailon. Kor. J. Soc. Hygienic Sciences. 10: 227-231.
  17. Ryu, M. J., S. Y. Lee, Y. Park, and Y. K. Yang (2011) Antioxidative Activities and Antifungal Effect against Malassezia furfur in the Extracts from 6 spp. Medicinal Plants. J. Kor. Soc. Cosm. 16: 120-128.
  18. Piddok, L. J. V. (1990) Techniques used for the determination of antibacterial resistence and sensitivity in bacteria. J. Appl. Bacteriol. 68: 307-318. https://doi.org/10.1111/j.1365-2672.1990.tb02880.x
  19. Cateni, F., J. Zilic, M. Zacchigna, P. Bonivento, F. Frausin, and V. Scarcia (2006) Synthesis and biological properties of new $\alpha$-methylene-$\gamma$-butyrolactones and $\alpha$,$\beta$‚-unsaturated delta-lactones. Eur. J. Med. Chem. 41: 192-200. https://doi.org/10.1016/j.ejmech.2005.10.009
  20. Matsuda, H., T. Kagerura, I. Toguchida, H. Ueda, T. Morikawa, and M. Yoshikawa (2000) Inhibitory effects of sesquiterpenes from bay leaf on nitric oxide production in lipopolysaccharideactivated macrophages: structure requirement and role of heat shock protein induction. Life Sci. 66: 2151-2157. https://doi.org/10.1016/S0024-3205(00)00542-7
  21. Hoffmann, H. M., and J. Rabe (1985) Synthesis and Biological activity of $\alpha$-methylene-$\gamma$-butyrolactones. Angew. Chem. Int. Ed. Engl. 24: 94-110. https://doi.org/10.1002/anie.198500941

피인용 문헌

  1. Hemistepsin A Inhibits Cell Proliferation and Induces G0/G1-Phase Arrest, Cellular Senescence and Apoptosis Via the AMPK and p53/p21 Signals in Human Hepatocellular Carcinoma vol.10, pp.5, 2013, https://doi.org/10.3390/biom10050713