Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Screening of Rhizobium, Hairy Vetch Root Nodule Bacteria, with Promotion of Nodulation and Nitrogen Fixation

뿌리혹 형성능과 질소 고정능이 우수한 헤어리베치 유래 Rhizobium의 분리 및 선발

  • Jang, Jong-Ok (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwon, Mi-Kyung (Agricultural Environment Research Division, Chungcheongnam-do Agricultural Research and Extension Services) ;
  • Park, Dong-Jin (Korea Research Institute of Bioscience and Biotechnology) ;
  • Sung, Chang Keun (Department of Food Science and Technology, Chungnam National University) ;
  • Kim, Chang-Jin (Korea Research Institute of Bioscience and Biotechnology)
  • 장종옥 (한국생명공학연구원 미생물자원센터) ;
  • 권미경 (충청남도농업기술원 농업환경연구과) ;
  • 박동진 (한국생명공학연구원 미생물자원센터) ;
  • 성창근 (충남대학교 식품공학과) ;
  • 김창진 (한국생명공학연구원 미생물자원센터)
  • Received : 2013.03.26
  • Accepted : 2013.04.19
  • Published : 2013.06.30
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Abstract

This study was conducted to select rhizobia from hairy vetch (Vicia villosa Roth) with nodulation and excellent nitrogen-fixing ability. Hairy vetch root was collected from 7 of cultivation region of all over the country, rhizobia were isolated from the root nodules. Isolates were re-inoculated into a hairy vetch separately and studied nodulation and nitrogen-fixing ability. As a result, total of 52 Rhizobium isolates were isolated from the hairy vetch root nodules, among these, 16 isolates were Rhizobium which show good growth at more than 0.5% NaCl concentration. These 16 isolates were re-inoculated separately, 8 weeks after, good root nodule formation was observed from Rhizobium sp. RH1, RH3, RH81, RH82, RH84, and RH93 strain treated samples. Six isolates were positive for nitrogen fixing ability, the highest acetylene reduction activity was shown by Rhizobium sp. RH84. Results suggest that the Rhizobium sp. RH84 could be used as the possibility of its application as a green manure crop of hairy vetches in nonuniform salt distribution reclaimed land.

본 연구에서는 헤어리베치로부터 뿌리혹 형성능과 질소 고정능이 우수한 Rhizobium 균주를 분리하고자 하였다. 전국의 일곱 군데 헤어리베치 재배 지역에서 수집 후 뿌리혹으로부터 균주를 분리하였고, 헤어리베치에 분리 균주를 재접종하여 뿌리혹 형성능과 질소 고정능을 확인하였다. 그 결과, 52개의 Rhizobium 속 균주를 분리하였고, 이 중 0.5% NaCl 농도 이상에서 자라는 Rhizobium은 16균주이었다. 뿌리혹 형성능은 16개 균주 중 Rhizobium sp. RH1, RH3, RH81, RH82, RH84, RH93의 균주를 접종한 시험구에서 우수하였다. 또한, 질소 고정능을 확인한 결과 Rhizobium sp. RH84가 가장 높은 acetylene reduction activity를 보였다. 이러한 결과는 향후 염의 분포가 불균일한 간척지에 적용 시에도 헤어리베치의 생육을 촉진해 녹비 작물로의 활용 가능성을 높여주는 결과로 판단된다.

Keywords

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