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http://dx.doi.org/10.7845/kjm.2013.3024

Screening of Rhizobium, Hairy Vetch Root Nodule Bacteria, with Promotion of Nodulation and Nitrogen Fixation  

Jang, Jong-Ok (Korea Research Institute of Bioscience and Biotechnology)
Kwon, Mi-Kyung (Agricultural Environment Research Division, Chungcheongnam-do Agricultural Research and Extension Services)
Park, Dong-Jin (Korea Research Institute of Bioscience and Biotechnology)
Sung, Chang Keun (Department of Food Science and Technology, Chungnam National University)
Kim, Chang-Jin (Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Korean Journal of Microbiology / v.49, no.2, 2013 , pp. 131-136 More about this Journal
Abstract
This study was conducted to select rhizobia from hairy vetch (Vicia villosa Roth) with nodulation and excellent nitrogen-fixing ability. Hairy vetch root was collected from 7 of cultivation region of all over the country, rhizobia were isolated from the root nodules. Isolates were re-inoculated into a hairy vetch separately and studied nodulation and nitrogen-fixing ability. As a result, total of 52 Rhizobium isolates were isolated from the hairy vetch root nodules, among these, 16 isolates were Rhizobium which show good growth at more than 0.5% NaCl concentration. These 16 isolates were re-inoculated separately, 8 weeks after, good root nodule formation was observed from Rhizobium sp. RH1, RH3, RH81, RH82, RH84, and RH93 strain treated samples. Six isolates were positive for nitrogen fixing ability, the highest acetylene reduction activity was shown by Rhizobium sp. RH84. Results suggest that the Rhizobium sp. RH84 could be used as the possibility of its application as a green manure crop of hairy vetches in nonuniform salt distribution reclaimed land.
Keywords
Rhizobium sp.; hairy vetch; nitrogen fixation; root nodule;
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Times Cited By KSCI : 7  (Citation Analysis)
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