Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Inhibitory Effect of Scolopendra Morsitans L. Ether Extract on Lipopolysaccharide-Induced Inflammatory Response in RAW 264.7 Cells

오공(蜈蚣) 에테르 추출물의 RAW 264.7 cell에서 LPS로 유도된 염증반응 억제 효과

  • Jung, Ho Kyung (Jeonnam Development Institute for Korean Traditional Medicine) ;
  • Cho, Hyun Woo (Jeonnam Development Institute for Korean Traditional Medicine) ;
  • Jung, Won Seok (Jeonnam Development Institute for Korean Traditional Medicine) ;
  • Choi, In Young (Jeonnam Development Institute for Korean Traditional Medicine) ;
  • Cho, Jung Hee (Jeonnam Development Institute for Korean Traditional Medicine) ;
  • Jung, Su Young (Jeonnam Development Institute for Korean Traditional Medicine)
  • 정호경 (전라남도한방산업진흥원) ;
  • 조현우 (전라남도한방산업진흥원) ;
  • 정원석 (전라남도한방산업진흥원) ;
  • 최인영 (전라남도한방산업진흥원) ;
  • 조정희 (전라남도한방산업진흥원) ;
  • 정수영 (전라남도한방산업진흥원)
  • Received : 2012.10.22
  • Accepted : 2013.01.14
  • Published : 2013.02.25
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Abstract

It has been reported that Scolopendra morsitans L.(SML) has beneficial effects on human health and diverse diseases. The purpose of this study was to investigate the anti-inflammatory effects of ether extract from Scolopendra morsitans L. on lipopolysaccharide(LPS)-induced inflammatory response. Thus, we examined the inhibitory effect of SML ether fraction on LPS-induced increase of inflammatory mediators(NO, iNOS, COX-2, and $I{\kappa}B{\alpha}$) and pro-inflammatory cytokines(TNF-${\alpha}$) in RAW 264.7 cells. In the present study, SML ether extract itself decreased cell viability in a dose dependent manner(> 100 ${\mu}g/ml$). In addition, LPS increased NO production, iNOS expression and phosphorylation of $I{\kappa}-B{\alpha}$, which were blocked by the treatment of SML ether fraction in a dose dependent manner. Furthermore, the treatment of LPS increased TNF-${\alpha}$ production. However, the pretreatment of SML ether fraction prevented the LPS-induced TNF-${\alpha}$ production in dose dependant manner. Taken together, our results suggest that SML may be a beneficial drug against inflammatory diseases such as sepsis.

Keywords

References

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