Anticancer and Related Immunomodulatory and Anticachexic Effects of Insamyangyoung-tang Extracts on Non Small Cell Lung Carcinoma, NCI-H520, Xenograft Mice

인삼양영탕(人蔘養榮湯)이 비소세포 폐암세포 이식 마우스에서 항암, 면역활성 및 악액질 억제에 미치는 영향

  • Song, Kwang Kyu (Haein Couple Korean Clinic) ;
  • Park, Mee Yeon (Division of Intenal Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Choi, Hae Yun (Division of Intenal Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Jong Dae (Division of Intenal Medicine, College of Korean Medicine, Daegu Haany University)
  • 송광규 (해인부부한의원) ;
  • 박미연 (대구한의대학교 한의학과 내과학교실) ;
  • 최해윤 (대구한의대학교 한의학과 내과학교실) ;
  • 김종대 (대구한의대학교 한의학과 내과학교실)
  • Received : 2012.10.26
  • Accepted : 2013.01.08
  • Published : 2013.02.25

Abstract

The object of this study was to observe anticancer and related immunomodulatory and anticachexic effects of Insamyangyoung-tang aqueous extracts (ISYYTe) on non-small cell lung carcinoma (squamous epithelial carcinoma), NCI-H520, xenograft Balb/c nu-nu nude mice. Changes on the tumor volume and weights, lymphatic organ(spleen and popliteal lymph node), serum interferon (IFN)-${\gamma}$ levels, splenocytes and peritoneal macrophage activities (NK cell activity), splenic tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$ and IL-10 contents, splenic T-lymphocyte subsets (CD3+, CD4+ and CD8+) and TNF-${\alpha}+$ cells were observed with tumor mass and lymphatic organ histopathology to detect anticancer and immunomodulatory effects. In addition, changes on the body weights, epididymal fat weights and serum IL-6 levels were also detected with the thicknesses of deposited cervical brown adipose tissue and their mean diameters to monitor the tumor-related anticachexic effects. The results obtained in this study suggest that over 50 mg/kg of ISYYTe showed favorable anticancer effects on the NCI-H520 cell xenograft with immunomodulatory and anticachexic effects. However, detail mechanism studies should be conducted in future with the screening of the biological active compounds in this herb.

Keywords

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