Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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The systematic consideration of leaf epidermal microstructure in the tribe Forsythieae and its related genera (Oleaceae)

개나리족 및 근연분류군(물푸레나무과)의 잎 표피 미세구조에 대한 분류학적 검토

  • Song, Jun-Ho (Laboratory of Plant Systematics, Department of Biology & Research Institute for Basic Sciences, Kyung Hee University) ;
  • Hong, Suk-Pyo (Laboratory of Plant Systematics, Department of Biology & Research Institute for Basic Sciences, Kyung Hee University)
  • 송준호 (경희대학교 이과대학 생물학과 및 기초과학연구소) ;
  • 홍석표 (경희대학교 이과대학 생물학과 및 기초과학연구소)
  • Received : 2013.05.01
  • Accepted : 2013.06.07
  • Published : 2013.06.30
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Abstract

A comparative study of leaf epidermal microstructure in tribes Forsythieae (Abeliophyllum: 1 species, Forsythia: 12 taxa - 10 species, 2 varieties), Fontanesieae (Fontanesia: 2 species) including one related genus Myxopyrum belonging to Myxopyreae (Myxopyrum: 5 taxa - 4 species, 1 subspecies) was carried out using scanning electron microscopy (SEM) in order to evaluate their significance in the taxonomy. The leaves of investigated taxa are either hypostomatic or amphistomatic, but former is more frequent. The size range of the guard cells is 17.14-47.58 ${\times}$ 11.59- 44.25 ${\mu}m$: the smallest one was found in Forsythia giraldiana (17.48-22.96 ${\times}$ 11.64-12.88 ${\mu}m$), while the largest one was measured to Myxopyrum pierrei (31.50-41.75 ${\times}$ 32.53-44.25 ${\mu}m$). Anomocytic stomatal complex are most frequent type (rarely paracytic), usually both anomocytic and anisocytic occur in one leaf. In surface view both adaxial and abaxial anticlinal walls of the subsidiary cells are variable (e.g., straight/curved, undulate, sinuate, undulate/sinuate). Three types (simple unicellular and multicellular non-glandular, subsessile glandular) of trichomes are found in leaves. Finally, the systematic significance of the leaf epidermal micromorphological characters in idenfitication and elucidation of these tribe, between or within the genera including among the species is also briefly discussed.

개나리족(Abeliophyllum: 1종, Forsythia: 12분류군-10종, 2변종)과 향선나무족(Fontanesia: 2 종) 및 근연 분류군인 Myxopyreae족(Myxopyrum: 5분류군-4종, 1아종)을 포함한 총 20분류군의 잎 표피 미세구조에 대한 분류학적 유용성을 검토하고자 주사전자현미경(SEM)으로 관찰하고 기재하였다. 기공복합체(stomatal complex)는 대부분의 분류군에서 배축면(abaxial side)에만 존재하는 이면기공엽(hypostomatic type)이었으나 일부 분류군에서는 잎의 양면에 모두 존재하는 양면기공엽(amphistomatic type)으로 나타났다. 공변세포의 크기는 17.14-47.58 ${\times}$ 11.59-44.25 ${\mu}m$으로 속과 종마다 다소 차이를 보이는데, Forsythia giraldiana (17.48-22.96 ${\times}$ 11.64-12.88 ${\mu}m$)가 가장 작은 공변세포를 지니고 있었고, Myxopyrum pierrei (31.50-41.75 ${\times}$ 32.53-44.25 ${\mu}m$)에서 가장 크게 나타났다. 기공복합체의 형태는 대부분 불규칙형(anomocytic)이 우세하며, 불규칙형과 불균등형(anisocytic)이모두 나타나거나 드물게 평행형(paracytic)이 나타나는 분류군이 확인되었다. 부세포의 수층벽(anticlinal wall)은 직선형과 곡선형이 동시에 관찰(straight/curved) 되거나 파상형(undulate) 또는 굴곡형(sinuate)이 나타나며, 파상형과 굴곡형이 동시에 나타나는 경우(undulate/sinuate)로 구분되었다. 연구된 분류군에서 나타나는 모용의 종류는 모두 3종류로 단세포 비선모(simple unicellular non-glandular trichome), 다세포 비선모(simple multicellular nonglandular trichome), 방패형 선모(subsessile/peltate glandular trichome)가 나타났다. 끝으로 조사된 분류군내의 속간, 종간 동정을 위한 잎 표피 미세구조학적 형질의 유용성 및 분류학적 적용 가능성을 검토하였다.

Keywords

References

  1. Airy Shaw, H. K. 1952. Note on the taxonomic position of Nyctanthes L. and Dimetra Kerr. Kew Bulletin 2: 271-272.
  2. Baranova, M. 1972. Systematic anatomy of the leaf epidermis in the Magnoliaceae and some related families. Taxon 21: 447-469. https://doi.org/10.2307/1219106
  3. Bass, P., P. M. Esser, M. E. T. Van Der Waster and M. Zandee. 1988. Wood anatomy of the Oleaceae. International Association of Wood Anatomists Bulletin 9: 103-182.
  4. Bini Maleci, L. and O. Servettaz. 1991. Morphology and distribution of trichomes in Italian species of Teucrium sect. Chamaedrys (Labiatae) - a taxonomical evaluation. Plant Systematics and Evolution 174: 83-91. https://doi.org/10.1007/BF00937696
  5. Chang, M. C., L. Q. Qiu and P. S. Green. 1996. Oleaceae. In Flora of China. Vol. 15. Wu, Z. Y., P. H. Raven and D. Y. Hong (eds.). Science Press and Missouri Botanical Garden Press, Beijing and St. Louis. Pp. 272-319.
  6. Darlington, C. D. and A. P. Wylie. 1955. Chromosome atlas of Flowering Plants. George Allen and Uniwin, London.
  7. Esau, K. 1977. Anatomy of Seed Plants. 2nd (ed). John Wiley and Sons, New York.
  8. Goldblatt, P. and D. E. Johnson. 2008. Index to Plant Chromosome Numbers 2004-2006. http://www.tropicos.org/Project/IPCN.
  9. Green, P. S. 1972. Forsythia. In Flora Europaea. Vol 3. Tutin, T. G., V. H. Heywood, N. A. Burges, D. M. Moore, D. H. Valentine, S. M. Walers and D. A. Webb (eds.). Cambridge University Press, Cambridge. Pp. 53.
  10. Green, P. S. 2004. Oleaceae. In Flowering plants, Dicotyledons: Lamiales (except Acanthaceae including Avicenniaceae). Vol 7. Kadereit, J. W. (ed.). Springer-Verlag, New York. Pp. 296-306.
  11. Han, B. W. 2001. The Palynological study of the Oleaceae. M.S. Thesis of Sungkyunkwan University, Suwon.
  12. Holmgren, P. K. and N. H. Holmgren. 1998. Index Herbariorum. Retrieved Mar. 20, 2013, from http://sciweb.nybg.org/science2/IndexHerbariorum.asp.
  13. Iwatsuki, K., T. Yamazaki, D. E. Boufford and H. Ohba. 1993. Flora of Japan: Vol. IIIa Angiospermae. Dicotyledoneae. Sympetalae (a). Kodansha, Tokyo. Pp. 122-135.
  14. Jensen, S. R. 1992. Systematic implications of the distribution of iridoids and other chemical compounds in the Loganiaceae and other families of the Asteridae. Annals of the Missouri Botanical Garden 79: 284-302. https://doi.org/10.2307/2399770
  15. Jensen, S. R., H. Franzyk and E. Wallander. 2002. Chemotaxonomy of the Oleaceae: iridoids as taxonomic markers. Phytochemistry 60: 213-231. https://doi.org/10.1016/S0031-9422(02)00102-4
  16. Johnson, L. A. S. 1957. Review of the family Oleaceae. Contributions from the New South Wales National Herbarium 2: 395-418.
  17. Kiew, R. 1984. The genus Myxopyrum L. (Oleaceae). Blumea 29: 499-512.
  18. Kim, D. K. and J. H. Kim. 2011. Molecular phylogeny of tribe Forsythieae (Oleaceae) based on nuclear ribosomal DNA internal transcribed spacers and plastid DNA trnL-F and matK gene sequences. Journal of Plant Research 124: 339-347. https://doi.org/10.1007/s10265-010-0383-9
  19. Kim, K. J. 1998. A new species of Fontanesia (Oleaceae) from China and taxonomic revision of the genus. Journal of Plant Biology 41: 142-145. https://doi.org/10.1007/BF03030401
  20. Kim, K. J. 1999. Molecular phylogeny of Forsythia based on chloroplast DNA variation. Plant Systematics and Evolution 218: 113-123. https://doi.org/10.1007/BF01087039
  21. Kim, K. J., H. L. Lee and Y. D. Kim. 2000. Phylogenetic position of Abeliophyllum based on nuclear ITS sequence data. Korean Journal of Plant Taxonomy 30: 235-250. (in Korean) https://doi.org/10.11110/kjpt.2000.30.3.235
  22. Lee, S. 1984. A systematic study of Korean Forsythia species. Korean Journal of Plant Taxonomy 14: 87-107. (in Korean) https://doi.org/10.11110/kjpt.1984.14.2.087
  23. Lee, S. 2011. Palynological contributions to the taxonomy of family Oleaceae, with special empahsis on genus Forsythia (tribe Forsytheae). Korean Journal of Plant Taxonomy 41: 175-181. https://doi.org/10.11110/kjpt.2011.41.3.175
  24. Lee, S. and E. J. Park. 1982a. A cladistic analysis of the Korean Oleaceae. Journal of Plant Biology 25: 57-64. (in Korean)
  25. Lee, S. and E. J. Park. 1982b. A palynotaxonomic study of the Korean Oleaceae. Journal of Plant Biology 25: 1-11. (in Korean)
  26. Lee, T. B. 1976. New forms of Abeliophyllum distichum. Korean Journal of Plant Taxonomy 7: 21-22. (in Korean) https://doi.org/10.11110/kjpt.1976.7.1.021
  27. Lim, S. C. and S. C. Ko. 1989. A cytotaxonomical study on some species of Korean Forsythia. Korean Journal of Plant Taxonomy 19: 229-239. (in Korean) https://doi.org/10.11110/kjpt.1989.19.4.229
  28. Mabberley, D. J. 2008. Mabberley's Plant-book. A Portable Dictionary of Plants, their Classifications, and Uses. 3rd (ed). University of Washington Botanic Gardens, Seattle.
  29. Meakawa, F. 1962. Major polyploid with special reference to the phylogeny of Oleaceae. Journal of Japanese Botany. 37: 25-27.
  30. Moon H. K. and S. P. Hong. 2003. The taxonomic consideration of leaf epidermal microstructure in Lycopus L. (Mentheae, Lamiaceae). Korean Journal of Plant Taxonomy 33: 151-164. (in Korean) https://doi.org/10.11110/kjpt.2003.33.2.151
  31. Moon, H. K., S. P. Hong, E, Smets and S. Huysmans. 2009. Phylogenetic significance of leaf micromorphology and anatomy in the tribe Mentheae (Nepetoideae: Lamiaceae). Botanical Journal of the Linnean Society 160: 211-231. https://doi.org/10.1111/j.1095-8339.2009.00979.x
  32. Nakai, T. 1919. Genus novum Oleacearum in Corea media inventum. The botanical magazine. Tokyo. 33: 153-154. https://doi.org/10.15281/jplantres1887.33.392_153
  33. Nakai, T. 1920. On Abeliophyllum distichum. The botanical magazine. Tokyo. 34: 249-251.
  34. Navarro, T. and J. El Oualidi. 2000. Trichome morphology in Teucrium L. (Labiatae). A taxonomic review. Anales del Jardin Botanico de Madrid 57: 277-297.
  35. O'Mara, J. 1930. Chromosome numbers in the genus Forsythia. Journal of the Arnold Arboretum 11: 14-15.
  36. Rehder, A. 1940. Manual of cultivated trees and shrubs hardy in North America. Macmillan Publishing Co., New York. Pp. 765-793.
  37. Rohwer, J. G. 1996. Die Frucht und Samenstrukturen der Oleaceae. Bibliotheca Botanica 148: 1-177.
  38. Sax, K. and E. C. Abbe. 1932. Chromosome numbers and the anatomy of the secondary xylem in the Oleaceae. Journal of the Arnold Arboretum 13: 37-47.
  39. Sehr, E. M. and A. Weber. 2009. Floral ontogeny of Oleaceae and its systematic implications. International Journal of Plant Sciences 170: 845-859. https://doi.org/10.1086/599074
  40. Song, J. H. and S. P. Hong. 2012. Comparative petiole anatomy in Forsythieae, Fontanesieae and Myxopyrum (Oleaceae) and its systematic implication. Korean Journal of Plant Taxonomy 42: 50-63. (in Korean) https://doi.org/10.11110/kjpt.2012.42.1.050
  41. Stace, C. A. 1984. The taxonomic importance of the leaf surface. In Current Concepts in Plant Taxonomy. Heywood, V. H. and D. M. Moore (eds.). Academic Press, London. Pp. 67-93.
  42. Tae, K. H., D. K. Kim and J. H. Kim. 2005a. Genetic variations and phylogenetic relationships of tribe Forsythieae (Oleaceae) based on RAPD analysis. Korean Journal of Plant Resources 8: 135-144.
  43. Tae, K. H., J. H. Tho and J. H. Kim. 2005b. A systematic study of Abeliophyllum distichum (Oleaceae) based on cytological characters. Korean Journal of Plant Taxonomy 35: 143-151. (in Korean) https://doi.org/10.11110/kjpt.2005.35.2.143
  44. Taylor, H. 1945. Cyto-taxonomy and phylogeny of Oleaceae. Brittonia 5: 337-369. https://doi.org/10.2307/2804889
  45. Wallander, E. and V. A. Albert. 2000. Phylogeny and classification of Oleaceae based on rps16 and trnL-F sequence data. American Journal of Botany 87: 1827-1841. https://doi.org/10.2307/2656836
  46. Weryszko-Chmielewska, E. and M. Chernetskyy. 2005. Structure of trichomes from the surface of leaves of some species of Kalanchoe Adans. Acta Biologica Cracoviensia Series Botanica 47: 15-22.
  47. Wilkinson, H. P. 1979. The plant surface (mainly leaf). In Anatomy of the Dicotyledons. 2nd (ed). Vol. I. Metcalfe, C. R. and L. Chalk (eds.). Clarendon Press, Oxford. Pp. 97-165.

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