Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Optimal Derivation Timing for Establishment of Porcine Embryonic Stem Cells

돼지 배아줄기세포 확립을 위한 최적의 유도시기

  • Kim, Eun-Hye (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University) ;
  • Cheong, Seung-A (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University) ;
  • Yoon, Junchul David (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University) ;
  • Jeon, Yubyeol (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University) ;
  • Hyun, Sang-Hwan (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University)
  • 김은혜 (충북대학교 수의과대학 수의학과) ;
  • 정승아 (충북대학교 수의과대학 수의학과) ;
  • 윤준철 (충북대학교 수의과대학 수의학과) ;
  • 전유별 (충북대학교 수의과대학 수의학과) ;
  • 현상환 (충북대학교 수의과대학 수의학과)
  • Received : 2013.02.28
  • Accepted : 2013.03.09
  • Published : 2013.03.31
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Abstract

A lot of works have been dedicated to clarify the reasons why the establishment of embryonic stem cells (ESCs) from pig is more difficult than that from mouse and human. Several concomitant factors such as culture condition including feeder layer, sensitivity of cell to cell contact, definitive markers of pluripotency for evaluation of the validity and optimal timing of derivation have been suggested as the disturbing factors in the establishment of porcine ESCs Traditionally, attempts to derive stem cells from porcine embryos have depend on protocols established for mouse ESCs using inner cell mass (ICM) for the isolation and culture. And more recently, protocols used for primate ESCs were also applied. However, there is no report for the establishment of porcine ESCs. Indeed, ungulate species including pigs have crucial developmental differences unlike rodents and primates. Here we will review recent studies about issues for establishment of porcine ESCs and discuss the promise and strategies focusing on the timing for derivation and pluripotent state of porcine ESCs.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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