The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Roots of Daucus carota sativa abrogates acute phase of Inflammation by the Inhibition of NO and Pro-Inflammatory Cytokine Production

NO와 Pro-Inflammatory Cytokine의 억제를 통한 호라복(胡蘿蔔)의 항염증효과

  • Lee, Dong-Jin (Department of opthalmology and otolaryngology and dermatology, College of Korean Medicine, Daegu Haany University) ;
  • Park, Sang-Mi (College of Korean Medicine, Daegu Haany University) ;
  • Hwangbo, Min (Department of opthalmology and otolaryngology and dermatology, College of Korean Medicine, Daegu Haany University) ;
  • Jung, Tae-Young (College of Korean Medicine, Daegu Haany University) ;
  • Kim, Sang-Chan (College of Korean Medicine, Daegu Haany University) ;
  • Jee, Seon-Young (Department of opthalmology and otolaryngology and dermatology, College of Korean Medicine, Daegu Haany University)
  • 이동진 (대구한의대학교 안이비인후피부과학교실) ;
  • 박상미 (대구한의대학교 방제학교실) ;
  • 황보민 (대구한의대학교 안이비인후피부과학교실) ;
  • 정태영 (대구한의대학교 진단학교실) ;
  • 김상찬 (대구한의대학교 방제학교실) ;
  • 지선영 (대구한의대학교 안이비인후피부과학교실)
  • Received : 2013.04.11
  • Accepted : 2013.05.10
  • Published : 2013.05.25
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Abstract

Objectives : Daucus carota sativa has been frequently used as food supplements in many of the Asian countries, and a nutritional medical drug in traditional medicine. This research investigated the effects of Daucus carota sativa extract (DCE) on acute phases of inflammation in Raw 264.7 cells treated with lipopolysaccharide (LPS) in terms of the inhibition of nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$) and pro-inflammatory cytokines production. Methods : NO, $PGE_2$, tumor necrosis factor (TNF)-${\alpha}$, interleukin-$1{\beta}$ and interleukin-6 contents were assayed by ELISA, and expressions of inflammation-related proteins such as inducible NO synthase (iNOS) were determined by immunoblot analyses. Results : DCE treatment attenuated the LPS ability to increase the productions of NO and $PGE_2$ as well as the protein level of iNOS in a concentration-dependent manner. Consistently, treatment of the cells with DCE suppressed the production of TNF-${\alpha}$, interleukin-$1{\beta}$ and interleukin-6. DCE also caused decreases of inhibitor of ${\kappa}B{\alpha}$ phosphorylation induced by LPS in the cells, which means DCE inhibition of NF-${\kappa}B$ activity. Furthermore, DCE blocked LPS-induced phosphorylation of p38 and SAPK/JNK. Conclusion : This study showing here may be of help to understand the action mechanism of DCE, and provide the information for the medical use of Daucus carota sativa for the inflammatory disease.

Keywords

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