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http://dx.doi.org/10.6114/jkood.2013.26.2.045

Roots of Daucus carota sativa abrogates acute phase of Inflammation by the Inhibition of NO and Pro-Inflammatory Cytokine Production  

Lee, Dong-Jin (Department of opthalmology and otolaryngology and dermatology, College of Korean Medicine, Daegu Haany University)
Park, Sang-Mi (College of Korean Medicine, Daegu Haany University)
Hwangbo, Min (Department of opthalmology and otolaryngology and dermatology, College of Korean Medicine, Daegu Haany University)
Jung, Tae-Young (College of Korean Medicine, Daegu Haany University)
Kim, Sang-Chan (College of Korean Medicine, Daegu Haany University)
Jee, Seon-Young (Department of opthalmology and otolaryngology and dermatology, College of Korean Medicine, Daegu Haany University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.26, no.2, 2013 , pp. 45-57 More about this Journal
Abstract
Objectives : Daucus carota sativa has been frequently used as food supplements in many of the Asian countries, and a nutritional medical drug in traditional medicine. This research investigated the effects of Daucus carota sativa extract (DCE) on acute phases of inflammation in Raw 264.7 cells treated with lipopolysaccharide (LPS) in terms of the inhibition of nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$) and pro-inflammatory cytokines production. Methods : NO, $PGE_2$, tumor necrosis factor (TNF)-${\alpha}$, interleukin-$1{\beta}$ and interleukin-6 contents were assayed by ELISA, and expressions of inflammation-related proteins such as inducible NO synthase (iNOS) were determined by immunoblot analyses. Results : DCE treatment attenuated the LPS ability to increase the productions of NO and $PGE_2$ as well as the protein level of iNOS in a concentration-dependent manner. Consistently, treatment of the cells with DCE suppressed the production of TNF-${\alpha}$, interleukin-$1{\beta}$ and interleukin-6. DCE also caused decreases of inhibitor of ${\kappa}B{\alpha}$ phosphorylation induced by LPS in the cells, which means DCE inhibition of NF-${\kappa}B$ activity. Furthermore, DCE blocked LPS-induced phosphorylation of p38 and SAPK/JNK. Conclusion : This study showing here may be of help to understand the action mechanism of DCE, and provide the information for the medical use of Daucus carota sativa for the inflammatory disease.
Keywords
Daucus carota sativa; iNOS; TNF-${\alpha}$; Interleukin-$1{\beta}$; Interleukin-6; MAPK;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Dong C, Davis RJ, Flavell RA. MAP kinases in the immune response. Annu Rev Immunol. 2002;20:55-72.   DOI   ScienceOn
2 Mori M. Regulation of nitric oxide synthesis and apoptosis by arginase and arginine recycling. J Nutr. 2007;137:1616S-20S.
3 Posadas I, Terencio MC, Guillen I, Ferrandiz ML, Coloma J, Paya M, et al. Co-regulation between cyclo-oxygenase-2 and inducible nitric oxide synthase expression in the time-course of murine inflammation. Naunyn Schmiedebergs Arch Pharmacol. 2000;361: 98-106.   DOI   ScienceOn
4 Yun HY, Dawson VL, Dawson TM. Neurobiology of nitric oxide. Crit Rev Neurobiol. 1996;10:291-316.   DOI   ScienceOn
5 Weisz A, Cicatiello I, Esumi H. Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferon-gamma, bacterical lipopolysaccharide and NG- monomethyl- L-arginene. Biochem J. 1996;316:209-15.   DOI
6 McCartney-Francis N, Allen JB, Mizel DE, Albina JE, Xie QW, Nathan CF, et al. Suppression of arthritis by an inhibitor of nitic oxide synthase. J Exp Med. 1993; 178:749-54.   DOI   ScienceOn
7 Seo WG, Pae HO, Oh GS, Chai KY, Yun YG, Kwon TO, et al. Inhibitory effects of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon and lipopolysaccharide. Gen Pharmacol. 2000;35:21-8.   DOI   ScienceOn
8 Chiou WF, Chou CJ, Chen CF. Camptothecinsuppresses nitric oxide biosynthesis in RAW264.7 macrophages. Life Sci. 2001;69:625-35.   DOI   ScienceOn
9 Lee BG, Kim SH, Zee OP, Lee KR, Lee HY, Han JW, et al. Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two-carboline alkaloids extracted from Melia azedarach. Eur J Pharmacol. 2000;406:301-9.   DOI   ScienceOn
10 Seo WG, Pae HO, Oh GS, Kim NY, Kwon TO, Shin MK, et al. The aqueous extract of Rhodiola sachalinensis root enhances the expression of inducible nitric oxide synthase gene in RAW 264.7 macrophages. J Ethnopharmacol. 2001;76:119-23.   DOI   ScienceOn
11 Kawamata H, Ochiai H, Mantani N, Terasawa K. Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated RAW 264.7 cells, a murine macrophage cell line. Am J Chin Med. 2000;28:217-26.   DOI   ScienceOn
12 Guha M, Mackman N. LPS induction of gene expression in human monocytes. Cell Signal. 2001;13:85-94.   DOI   ScienceOn
13 Wogensen L, Jensen M, Svensson P, Worsaae H, Welinder B, Nerup J. Pancreatic beta-cell function and interleukin-$1{\beta}$ in plasma during the acute phase response in patients with major burn injuries. Eur J Clin Invest. 1993;23:311-9.   DOI   ScienceOn
14 Han EJ, Roh SB, Bae SJ. Cytotoxicity of Daucus Carota L. on Various Cancer Cells. J Korean Soc Food Sci Nutr. 2000;29:153-60.   과학기술학회마을
15 Jeon EJ, Kim JS, Park YK, Kim TS, Kang MH. Protective Effect of Yellow-Green Vegetable Juices on DNA Damage in Chinese Hamster Lung Cell Using Comet Assay. The Korean Nutrition Society. 2003;36:24-31.   과학기술학회마을
16 Kim M, Kim M, Shin D, Song C, Lee H. Growth-inhibiting effects of vegetable extracts on Beneficial and Harmful Human Intestinal Bacteria. Agric Chem Biotechnol. 2001;44: 65-70.
17 Gilani AH, Shaheen F, Saeed SA. Cardiovascular Actions of Daucus carota. Arch Pharm Res. 1994;17:150-3.   DOI
18 Ha JL, Bae JS, Park MK, Kim YU, Ha SH, Bae JM, et al. Quantitative Analysis of Carotenoids in Carrot Cultivars Produced in Korea. Journal of the Environmental Sciences. 2009;18:1135-41.   DOI   ScienceOn
19 Jirik FR, Podor TJ, Hirano T, Kishimoto T, Loskutoff DJ, Carson DA, et al. Bacterial lipopolysaccharide and inflammatory mediators augment IL-6 secretion by human endothelial cells. J Immunol. 1989;142:144-7.
20 Chen F, Castranova V, Shi X. New insights into the role of nuclear factor-kappaB in cell growth regulation. Am J Pathol. 2001; 159:387-97.   DOI   ScienceOn
21 Kim CM, Shin MG, Ahn DG, Lee GS, Kang BS, Kang SS. The Chinese Herbal Dictionary. Seoul:Jeongdam. 1997;6124-6.
22 Kim TJ. Wild Flowers and Resources Plants in Korea (3). Seoul:SNU Press. 2008;451.
23 Roh SR, Kim DH. Anti-tumor Effect of Carrot(Docus carota L.) Extracts in the Human Lung Cancer Cell Line NCI-H1299. J East Asian Soc Dietary Life. 2002;12:289-98.   과학기술학회마을
24 Lee AK, Sung SH, Kim YC, Kim SG. Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-$\alpha$ and COX-2 expression by sauchinone effects on I-${\kappa}B{\alpha}$ phosphorylation, C/EBP and AP-1 activation. Brit J Pharmacol. 2003;139:11-20.   DOI   ScienceOn
25 Takabayashi T, Shimizu S, Clark BD, Beinborn M, Burke JF, Gelfand JA. Interleukin-1 upregulates anaphylatoxin receptors on mononuclear cells. Surgery. 2004;135:544-54.   DOI   ScienceOn
26 Noh MS, Ha JY, Lee CH, Lee WY, Lee SH, Lee JJ. Inhibitory activities of natural products on lipopolysaccharide induced prostaglandin production in mouse macrophages. Yakhak Hoeji. 1998;42:558-66.
27 Surh YJ. Role of Cyclooxygenase-2 in Carcimogenesis and Its Selective Inhibition for Cancer Chemoprevention. Molecules and cells. 2001;13:8-17.
28 Palombella VJ, Rando OJ, Goldberg AL, Maniatis T. The ubiquitinproteasome pathway is required for processing the NF-${\kappa}B1$ precursor protein and the activation of NF-${\kappa}B$. Cell. 1996;78:773-85.
29 Nathan C. Nitric oxide as a secretory product of mammalian cells. FASEB J. 1992;6:3051-64.
30 Grosch S, Maier TJ, Schiffmann S, Geisslinger G. Cyclooxygenase-2 (COX-2)-independent anticarcinogenic effects of selective COX-2 inhibitors. J Natl Cancer Inst. 2006;98:736-47.   DOI   ScienceOn
31 Sohn GH, Kim SH. Antitumor Activity of Bupleuri Radix and Artemisiae capillaris Herba and Synergistic Effect with Anticancer Drugs. KOMS. 1995;16:414-32.   과학기술학회마을
32 Corbett JA, Mac Daniel ML. Intraislet release of interleukin-1 inhibits beta cell function by inducing beta cell expression of inducible nitric oxide syntheses. J Exp Med. 1995; 181:559-68.   DOI   ScienceOn
33 Lee YS, Kim HS, Kim SK, Kim SD. IL-6 mRNA Expression in Mouse Peritoneal Macrophages and NIH3T3 Fibroblasts in Response to Candida albicans. J Microbiol Biotechnol. 2000;10:8-15.
34 Higuchi M, Higashi N, Taki H, Osawa T. Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanism acts as synergistically as the major cytolytic mechanism of activated macrophages. J Immunol. 1990;144:1425-31.
35 McDaniel ML, Kwon G, Hill JR, Marshall CA, Corbett JA. Cytokines and nitric oxides in islet inflammation and diabetes. Proc Soc Exp Biol Med. 1996;211:24-32.   DOI
36 Cetkovic-Cvrlje M, Eizirik DL. TNF and $IFN{\gamma}$ potentiate the deleterious effects of IL-$1{\beta}$ on mouse pancreatic islets mainly via generation of nitric oxide. Cytokine. 1994; 6:399-406.   DOI   ScienceOn
37 Kook YB. Effect of Hwangryunhaedok-tang on Blood Pressure and Renal Functions in Spontaneously Hypertensive Rats. J of Herbal Prescription. 2002;10:113-29.
38 Aeberli D, Oertle S, Mauron H, Reichenbach S, Jordi B, Villiger PM. Inhibition of the TNF-pathway: use of infliximab and etanercept as remission-inducing agents in cases of therapy-resistant chronic inflammatory disorders. Swiss Med Wkly. 2002;132:414-22.
39 Delgado AV, McManus AT, Chambers JP. Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance. P Neuropeptides. 2003;37:355-61.   DOI   ScienceOn
40 The Korean Society of Pathologists. Pathology. Seoul:KMS. 2007;76-108.