Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
권호 표지
DOI QR코드

DOI QR Code

Comparative Ultrastructures of the Fertilized Egg Envelopes in Danio rerio and Danio rerio var. frankei, Cyprinidae, Teleostei

  • Joo, Kyung Bok (Department of Ophthalmic Optics, Chodang University) ;
  • Kim, Dong Heui (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University)
  • Received : 2012.11.28
  • Accepted : 2013.03.04
  • Published : 2013.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

The leopard danio, Danio rerio var. frankei is a spotted color morph of the zebrafish, Danio rerio caused by a pigment mutation. The structural differences of fertilized egg and egg envelope are poorly documented. To clarify this, we compared the fertilized egg morphology and ultrastructures of surface structures, the micropyle and the cross section of fertilized egg envelopes of zebrafish and leopard danio, variation species of zebrafish using a light and electron microscopes. Although the fertilized egg sizes were different, the external shapes of the fertilized eggs of two species couldn't be differentiated under the light microscope. The characteristics of fertilized eggs, such as a spherical shape, a non-adhesive quality and a large perivitelline space, were shown to be related to spawning habit. In ultrastructure of fertilized egg envelope, there is no morphological difference of micropyle between two species. By contrast, the ultrastructure and the numbers of knob-like structures and semihemisphere-like structures per unit area on the outer surface, and the number of lamellae of inner layer on the fertilized egg envelope section displayed definite species specificity. Collectively, our data indicate that the ultrastructure of fertilized egg envelope in the zebrafish could be differentiated by species variation.

Keywords

References

  1. Brummett A R and Dumont J N (1981) A comparison of chorions from eggs of northern and southern populations of Fundulus heteroclitus. Copeia 3, 607-614.
  2. Cameron I L and Hunter K E (1984) Regulation of the permeability of the medaka fish embryo chorion by exogeneous sodium and calcium ions. J. Exp. Zool. 231, 447-454. https://doi.org/10.1002/jez.1402310320
  3. Cherr G N and Jr Clark W H (1984) An acrosome reaction in sperm from the white sturgeon, Acipenser transmontanus. J. Exp. Zool. 232, 129-139. https://doi.org/10.1002/jez.1402320116
  4. Deung Y K, Kim D H, and Reu D S (1999) Ultrastructure of gametes in the three-spine stickleback, Gasterosteus aculeatus aculeatus. Kor. J. Microscopy 29, 177-187.
  5. Deung Y K, Kim D H, and Reu D S (2000) Ultrastructure of the fertilized egg envelope from pale chub, Cyprinidae, teleost. Kor. J. Microscopy 30, 321-326.
  6. Deung Y K, Reu D S, and Kim D H (1997) Comparative ultrastructures of the fertilized egg envelopes in golden severum, convic cichlid and discus, Cichlidae, teleost. Kor. J. Microscopy 27, 417-432.
  7. Donovan M J and Hart N H (1986) Cortical granule exocytosis is coupled with membrane retrieval in the egg of Brachydanio. J. Exp. Zool. 237, 391-405. https://doi.org/10.1002/jez.1402370312
  8. Gilkey J C (1983) Roles of calcium and pH in activation of eggs of the medaka fish, Oryzias latipes. J. Cell Biol. 97, 669-678. https://doi.org/10.1083/jcb.97.3.669
  9. Grierson J P and Neville A C (1981) Helicoidal architecture of fish eggshell. Tissue Cell 13, 819-830. https://doi.org/10.1016/S0040-8166(81)80016-X
  10. Guraya S S (1986) The cell and molecular biology of fish oogenesis. In: Monographs in developmental biology, Vol. 18, ed. Sauer H W, pp. 110-147, (Karger, Basel).
  11. Gwo J C and Gwo H H (1993) Spermatogenesis in the Black Porgy, Acanthopagrus chlegeli (Teleostei, Perciformes, Sparidae). Mol. Reprod. Dev. 36, 75-83. https://doi.org/10.1002/mrd.1080360111
  12. Hart N H and Donovan M (1983) Fine structure of the chorion and site of sperm entry in the egg of Brachydanio. J. Exp. Zool. 227, 277-296. https://doi.org/10.1002/jez.1402270212
  13. Hart N H, Pietri R, and Donovan M (1984) The surface of the chorion and associated surface filaments in oryzias-evidence for the presence of extracellular tubules. J. Exp. Zool. 230, 273-296. https://doi.org/10.1002/jez.1402300213
  14. Harvey B, Kelley R N, and Ashwood-Smith M J (1983) Permeability of intact and dechorionated zebra fish embryos to glycerol and dimethyl sulfoxide. Cryobiol. 20, 432-439. https://doi.org/10.1016/0011-2240(83)90033-0
  15. Ivankov V N and Kurdyayeva V P (1973) Systematic differences and the ecological importance of the membranes in fish eggs. J. Ichthyol. 13, 864-873.
  16. Kille R A (1960) Fertilization of the lamprey egg. Exp. Cell Res. 20, 12-27. https://doi.org/10.1016/0014-4827(60)90218-4
  17. Kim D H, Chang B S, Teng Y C, Kim S, Joo K B, and Lee K J (2009) Ultrastructure of the fertilized egg envelope in Cichlasoma managuensis, Cichlidae, Teleost. Kor. J. Microscopy 39, 9-15.
  18. Kim D H, Deung Y K, Kim H Y, and Reu D S (2001) Ultrastructure of the fertilized egg envelope from long nose barbel, Cyprinidae, teleost. Kor. J. Microscopy 31, 85-90.
  19. Kim D H, Deung Y K, Kim W J, Reu D S, and Kang S J (1999) Comparative ultrastructures of the fertilized egg envelopes from three-spot gourami, pearl gourami and marble gourami, Belontiidae, teleost. Kor. J. Microscopy 29, 343-351.
  20. Kim D H, Reu D S, and Deung Y K (1996) A comparative study on the ultrastructures of the egg envelope in fertilized eggs of fishes, Characidae, three species. Kor. J. Microscopy 26, 277-291.
  21. Kim D H, Reu D S, and Deung Y K (1998a) Comparative ultrastructures of the fertilized egg envelopes in three species, Cyprinidae, teleost. Kor. J. Microscopy 28, 237-253.
  22. Kim D H, Reu D S, and Deung Y K (1998b) Ultrastructure of the fertilized egg envelope in tomato clown anemonefish (Amphiprion freatus), Pomacentridae, marine teleost. Kor. J. Microscopy 28, 273-282.
  23. Kim D H, Reu D S, and Deung Y K (2002) Ultrastructure of the fertilized egg envelope from dark sleeper, Eleotrididae, teleost. Kor. J. Microscopy 32, 39-44.
  24. Laale H W (1980) The perivitelline space and egg envelopes of bony fishes: a review. Copeia 2, 210-226.
  25. Lee K J, Chang B S, Teng Y C, Kim S, Song M S, Joo K B, and Kim D H (2009) The Spermatogenesis of Cichlasoma managuensis, Cichlidae, Teleost. Kor. J. Microscopy 39, 219-226.
  26. Lonning S and Davenport J (1980) The swelling egg of the long rough dab, Hippoglossoides platessoides limandoides (Bloch). J. Fish Biol. 17, 359-378. https://doi.org/10.1111/j.1095-8649.1980.tb02771.x
  27. Lonning S (1972) Comparative electron microscopic studies of teleostean eggs. Sarsia 49, 41-48.
  28. Meyer A, Biemann C H, and Orti G (1993) The phylogenetic position of the zebrafish (Danio rerio), a model system in developmental biology: an invitation to the comparative method. Proc. Biol. Sci. 252, 231-236. https://doi.org/10.1098/rspb.1993.0070
  29. Ohta T and Nashirozawa C (1996) Sperm penetration and transformation of sperm entry site in eggs of the freshwater teleost Rhodeus ocellatus ocellatus. J. Morphol. 229, 191-200. https://doi.org/10.1002/(SICI)1097-4687(199608)229:2<191::AID-JMOR4>3.0.CO;2-4
  30. Ohta T (1985) Electron microscopic observations on sperm entry into eggs of the bitterling during cross-fertilization. J. Exp. Zool. 233, 291- 300. https://doi.org/10.1002/jez.1402330218
  31. Schmehl M K and Graham E F (1987) Comparative ultrastructure of the zona radiata from eggs of six species of salmonids. Cell Tissue Res. 250, 513-519.
  32. Stanley H P (1971) Fine structure of spermiogenesis in the elasmobrach fish Squalus suckleyi. J. Ultrastruct. Res. 36, 86-102. https://doi.org/10.1016/S0022-5320(71)80090-4
  33. Stehr C M and Hawkes J W (1979) The comparative ultrastructure of the egg membrane and associated pore structures in the starry flounder, Platichthys stellatus (Pallas), and pink salmon, Oncorhynchus gorbuscha (Walbaum). Cell Tissue Res. 202, 347-356.
  34. Thiaw O T and Mattei X (1991) Morphogenesis of the secondary envelope of the oocyte in a teleostean fish of the family Cyprinodontidae: Aphyosemion splendopleure. J. Submicrosc. Cytol. Pathol. 23, 419-426.
  35. Watanabe M, Iwashita M, Ishii M, Kurachi Y, Kawakami A, Kondo S, and Okada N (2006) Spot pattern of leopard Danio is caused by mutation in the zebrafish connexin 41.8 gene. EMBO Rep. 7, 893-897. https://doi.org/10.1038/sj.embor.7400757
  36. Yamamoto T (1961) Physiology of fertilization in fish eggs. Int. Rev. Cytol. 12, 361-405.
  37. Yasumasu S, Katow S, Umino Y, Iuchi I, and Yamagami K (1989) A unique proteolytic action of HCE, a constituent protease of a fish hatching enzyme: tight binding to its natural substrate, egg envelope. Biochem. Biophys. Res. Commun. 162, 58-63. https://doi.org/10.1016/0006-291X(89)91961-X
  38. Yoon J M, Chung K Y, Reu D S, Lew I D, Roh S C, and Kim G W (1996) Electron microscopic observations on micropyle after sperm penetration in rainbow trout, Oncorhynchus mykiss. Kor. J. Zool. 39, 173-181.

Cited by

  1. (Hamilton, 1822) vol.31, pp.01758659, 2015, https://doi.org/10.1111/jai.12733
  2. , Nothobranchiidae, Teleostei vol.47, pp.2, 2017, https://doi.org/10.9729/AM.2017.47.2.70
  3. , Callichthyidae, Teleostei pp.1059910X, 2018, https://doi.org/10.1002/jemt.23057