Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effects of Dietary Supplementation of Spirulina and Quercetin on Growth, Innate Immune Responses, Disease Resistance Against Edwardsiella tarda, and Dietary Antioxidant Capacity in the Juvenile Olive Flounder Paralichthys olivaceus

  • Kim, Sung-Sam (Department of Marine Biomedical Science, Jeju National University) ;
  • Rahimnejad, Samad (Department of Marine Biomedical Science, Jeju National University) ;
  • Kim, Kang-Woong (Aquafeed Research Center, National Fisheries Research and Development Institute) ;
  • Lee, Bong-Joo (Aquafeed Research Center, National Fisheries Research and Development Institute) ;
  • Lee, Kyeong-Jun (Department of Marine Biomedical Science, Jeju National University)
  • Received : 2012.05.15
  • Accepted : 2012.12.26
  • Published : 2013.03.30
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Abstract

A 10-week feeding trial was conducted to examine the effects of dietary spirulina and quercetin on growth, innate immunity, disease resistance and dietary antioxidant capacity in the juvenile olive flounder. Triplicate groups of fish (initial body weight, $2.9{\pm}0.01g$) were fed one of isonitrogenous (48% crude protein) and isocaloric (17.4 MJ/kg DM) experimental diets containing 0% spirulina (as a control), 3.4% spirulina, or 6.8% spirulina with or without supplementation of 0.5% quercetin (designated as CON, SP3.4, SP6.8, and SP6.8 + Q, respectively) at a rate of 3% body mass twice daily. Higher dietary antioxidant capacity was found with spirulina supplementation, and the highest value (P < 0.05) was obtained with SP6.8 + Q diet. At the end of the feeding trial, no significant effects were observed on growth performance, body composition and disease resistance against Edwardsiella tarda. Lysozyme activity was significantly increased by spirulina supplementation (P < 0.05), and the highest value was observed in the group fed SP6.8 + Q diet. Also, significantly higher respiratory burst activity (P < 0.05) was found in SP3.4 group. According to the results of this study, dietary supplementation of 3.4% spirulina may enhance innate immunity of olive flounder.

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References

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