Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Quinone Reductase Induction Activity of Phlorotannins Derived from Eisenia bicyclis in Hepa1c1c7 Cells

  • Yoon, Na Young (Food and Safety Research Division, National Fisheries Research and Development Institute) ;
  • Lee, Sang-Hoon (Functional Materials Research Group, Korea Food Research Institute) ;
  • Shim, Kil Bo (Food and Safety Research Division, National Fisheries Research and Development Institute) ;
  • Lim, Chi-Won (Food and Safety Research Division, National Fisheries Research and Development Institute) ;
  • Lee, Moon-Hee (Food and Safety Research Division, National Fisheries Research and Development Institute) ;
  • Cho, Hyun-Ah (Food and Safety Research Division, National Fisheries Research and Development Institute) ;
  • Xie, Chengliang (Food and Safety Research Division, National Fisheries Research and Development Institute)
  • Received : 2012.08.28
  • Accepted : 2013.03.06
  • Published : 2013.03.30
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Abstract

To assess the feasibility of phlorotannins from Eisenia bicyclis as cancer chemopreventative agents, we tested whether they induced quinone reductase (QR) in Hepa1c1c7 cells. The ethyl acetate (EtOAc) soluble fraction obtained from E. bicyclis exhibited a QR induction activity in Hepa1c1c7 cells. Successive column chromatography of the active EtOAc fraction resulted in the isolation of four phlorotannins. Their structures were elucidated using one- and two-dimensional nuclear magnetic resonance spectroscopic techniques and characterized as phloroglucinol (1), dioxinodehydroeckol (2), dieckol (3), and fucofuroeckol-A (4). Among these compounds, fucofuroeckol-A (4) showed moderate QR induction activity, and dioxinodehydroeckol (2) exhibited potent QR induction potency with $2.05{\pm}0.04$ fold induction at a concentration of $50{\mu}M$ compared to the dimethyl sulfoxide solvent-treated control cells. However, phloroglucinol (1) and dieckol (3) exerted no detectable QR induction activity in Hepa1c1c7 cells. These results suggest that dioxinodehydroeckol could serve as a useful cancer chemopreventive chemical.

Keywords

References

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