DOI QR코드

DOI QR Code

DNA barcode analysis for conservation of an endangered species, Aporia crataegi (Lepidoptera, Pieridae) in Korea

멸종위기종, 상제나비(나비목, 흰나비과)의 보전을 위한 DNA 바코드 특성 분석

  • Park, Hae Chul (Applied Entomology Division, National Academy of Agricultural Science, RDA) ;
  • Han, Taeman (Applied Entomology Division, National Academy of Agricultural Science, RDA) ;
  • Kang, Tae Wha (Plant Quarantine Technology Venter, Animal and Plant Quarantine Agency) ;
  • Yi, Dae-Am (Yeongwol Insectarium Center for the Study of Insect Ecology) ;
  • Kim, Sung-Soo (Research Institute for East Asian Environment and Biology) ;
  • Lee, Young Bo (Applied Entomology Division, National Academy of Agricultural Science, RDA)
  • 박해철 (국립농업과학원 곤충산업과) ;
  • 한태만 (국립농업과학원 곤충산업과) ;
  • 강태화 (농림축산검역본부 식물검역기술개발센터) ;
  • 이대암 (영월곤충박물관 곤충자연생태연구소) ;
  • 김성수 (동아시아환경생물연구소) ;
  • 이영보 (국립농업과학원 곤충산업과)
  • Received : 2013.09.27
  • Accepted : 2013.10.22
  • Published : 2013.11.30

Abstract

Aporia crataegi, an Korean endangered species, was first analyzed for DNA barcode sequences based on 28-year-old dried specimens and compared barcode characters with 36 individuals of ten geographical populations of Eurasia. They were revealed to consist of five different haplotypes. Among them, haplotype I was mostly extensive and high frequency with 75%. The south Korean individuals were confirmed to be belonging to haplotype I and have no genetic isolation on COI gene. By these results, we consider that selection of the identical haplotype from other geographical populations may be a requirement prior to performing for conservation and restoration of the Korean population. We also propose to analyse the additional genetic markers in order to understand a more accurate genetic structures between haplotypes of this species.

멸종위기종인 남한산 상제나비의 28년 된 장기 건조표본을 이용하여 DNA 바코드 염기 서열을 최초로 분석하고, 이를 유라시아 10 지역 개체군 36개체들과 COI 특성을 비교해 보았다. 이들 개체군에서 총 5개의 haplotype을 확인하였고, haplotype I은 75%로 가장 높은 빈도를 나타내었으며, 유라시아 전 지역에 광범위하게 분포하고 있음을 확인하였다. 남한산 개체들은 모두 haplotype I에 속하고 있어 COI 유전자 상에서는 지역 고립성이 없는 것으로 밝혀졌다. 이 결과로 추후 남한산 상제나비 보전 및 복원은 타 지역 개체군 중 동일 haplotype 선별이 필수 요건으로 판단되나, 좀 더 정교한 평가를 위해서는 추가적인 마커를 이용한 분석이 필요할 것으로 제안한다.

Keywords

References

  1. Carnicer J, Stefanescu C, Vila R, Dinca V, Font X, Penuelas J (2013) A unified framework for diversity gradients: the adaptive trait continuum. Global Ecol Biogeo 22, 6-18. https://doi.org/10.1111/j.1466-8238.2012.00762.x
  2. Choi SW, Kim SS (2012) The past and current status of endangered butterflies in Korea. Ent Sci 15, 1-12. https://doi.org/10.1111/j.1479-8298.2011.00478.x
  3. Clement M, Posada D, Crandall K A (2000) TCS: a computer program to estimate gene geneologies. Mol Ecol 9, 1657-1659. https://doi.org/10.1046/j.1365-294x.2000.01020.x
  4. Dinca V, Zakharov EV, Hebert PDN, Vila R (2011) Complete DNA barcode reference library for a country's butterfly fauna reveals high performance for temperate Europe. Pro Royal Soc B 278, 347-355. https://doi.org/10.1098/rspb.2010.1089
  5. Hausmann A, Haszprunar G, Segerer AH, Speidel W, Behounek G, Hebert PDN (2011) Now DNA-barcoded: the butterflies and larger moths of Germany. Spixiana (Suppl.) 34, 47-58.
  6. Kim CW (1981) Insects: Rare and endangered species in Korea. pp 30-153. Korean Asso Conser Nature, Seoul.
  7. Kim HC (1992) Butterfly-fauna of Changwon-ri area in Yongwol-gun, Kangweon-do. Amateur Lep Soc Korea 5, 6-12.
  8. Kim SS, Seo MH (2012) Life histories of Korean Butterflies. Sakyejul Publishing Co.
  9. Kim YS, Hong SP (1990) Review of main butterflies species protected under Korean Law. Amateur Lep Soc Korea 3, 9-16.
  10. Korean Amateur Lepidopterist (1986) list of butterfies from Kyeonggido, Korea. Amateur Lep Soc Korea 1, 1-19.
  11. Korean Amateur Lepidopterist (1989) Butterfly-fauna of Kangwon-do, Korea. Amateur Lep Soc Korea 1, 1-19.
  12. Lukhtanov VA, Sourakov A, Zakharov EV, Hebert PDN (2009) DNA barcode central Asian butterflies: increasing geographical dimension does not significantly reduce the success of species identification. Mol Eco Res 9, 1302-1310. https://doi.org/10.1111/j.1755-0998.2009.02577.x
  13. Ministry of Environment (2005) Endangered plants and animals in Korea. Ministry of Environment, Seoul.
  14. Paek MK, Shin YH (2010) Butterflies of the Korean peninsula. In: Cho, YK(eds) Academic Series 1, Nature & Ecology press, Seoul.
  15. Park HC (2005) Endangered plants and animals: insect 4: Aporia. http://www.hani.co.kr/kisa/section-010003000/2005/12/010003000200512061709348.html.
  16. Park HC, Kim SS (2001) Study on current and new proposal of endangered plants and animals in Korea. Insects. pp 91-114. Ministry Enviro Kor Asso Conser Nat, Seoul.
  17. Park JS, Cho Y, Kim MJ, Nam SH, Kim I (2012) Description of complete mitochondrial genome of the black-veined white, Aporia crataegi (Lepidoptera: Papilionoidea), and comparison to papilionoid species. J Asia-Paci Ento 15, 331-341. https://doi.org/10.1016/j.aspen.2012.01.002
  18. Shapiro AM, Forister ML, Fordyce JA (2007) Extreme highaltitude asian and andean Pierid butterflies are not each others' closest relatives. Arc Anta Alp Rese 39, 137-142. https://doi.org/10.1657/1523-0430(2007)39[137:EHAAAP]2.0.CO;2
  19. Shin YH (1990) Study on survey of rare and threatened plants and animals in Korea. Insects. Bull Kor Asso Con Nat 10, 145-169.
  20. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Bio Evo 28, 2731-2739. https://doi.org/10.1093/molbev/msr121
  21. Tuzov VK (1997) Guide to the butterflies of Russia and adjacent territories. Pensoft Publishers, Sofia, Bulgaria and Moscow, Russia.
  22. Wahlberg N, Wheat CW (2008) Genomic outposts serve the phylogenomic pioneers: Designing novel nuclear markers for genomic DNA extractions of Lepidoptera. Sys Bio 57, 231-242. https://doi.org/10.1080/10635150802033006

Cited by

  1. Population genetic characterization of the black-veined white, Aporia crataegi (Lepidoptera: Pieridae), using novel microsatellite markers and mitochondrial DNA gene sequences vol.21, pp.2, 2013, https://doi.org/10.1007/s10592-020-01257-7