Browse > Article
http://dx.doi.org/10.7852/jses.2013.51.2.201

DNA barcode analysis for conservation of an endangered species, Aporia crataegi (Lepidoptera, Pieridae) in Korea  

Park, Hae Chul (Applied Entomology Division, National Academy of Agricultural Science, RDA)
Han, Taeman (Applied Entomology Division, National Academy of Agricultural Science, RDA)
Kang, Tae Wha (Plant Quarantine Technology Venter, Animal and Plant Quarantine Agency)
Yi, Dae-Am (Yeongwol Insectarium Center for the Study of Insect Ecology)
Kim, Sung-Soo (Research Institute for East Asian Environment and Biology)
Lee, Young Bo (Applied Entomology Division, National Academy of Agricultural Science, RDA)
Publication Information
Journal of Sericultural and Entomological Science / v.51, no.2, 2013 , pp. 201-206 More about this Journal
Abstract
Aporia crataegi, an Korean endangered species, was first analyzed for DNA barcode sequences based on 28-year-old dried specimens and compared barcode characters with 36 individuals of ten geographical populations of Eurasia. They were revealed to consist of five different haplotypes. Among them, haplotype I was mostly extensive and high frequency with 75%. The south Korean individuals were confirmed to be belonging to haplotype I and have no genetic isolation on COI gene. By these results, we consider that selection of the identical haplotype from other geographical populations may be a requirement prior to performing for conservation and restoration of the Korean population. We also propose to analyse the additional genetic markers in order to understand a more accurate genetic structures between haplotypes of this species.
Keywords
Lepidoptera; Pieridae; Aporia crataegi; DNA barcode; Haplotype; Conservation;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Tuzov VK (1997) Guide to the butterflies of Russia and adjacent territories. Pensoft Publishers, Sofia, Bulgaria and Moscow, Russia.
2 Carnicer J, Stefanescu C, Vila R, Dinca V, Font X, Penuelas J (2013) A unified framework for diversity gradients: the adaptive trait continuum. Global Ecol Biogeo 22, 6-18.   DOI   ScienceOn
3 Choi SW, Kim SS (2012) The past and current status of endangered butterflies in Korea. Ent Sci 15, 1-12.   DOI   ScienceOn
4 Clement M, Posada D, Crandall K A (2000) TCS: a computer program to estimate gene geneologies. Mol Ecol 9, 1657-1659.   DOI   ScienceOn
5 Dinca V, Zakharov EV, Hebert PDN, Vila R (2011) Complete DNA barcode reference library for a country's butterfly fauna reveals high performance for temperate Europe. Pro Royal Soc B 278, 347-355.   DOI   ScienceOn
6 Hausmann A, Haszprunar G, Segerer AH, Speidel W, Behounek G, Hebert PDN (2011) Now DNA-barcoded: the butterflies and larger moths of Germany. Spixiana (Suppl.) 34, 47-58.
7 Kim CW (1981) Insects: Rare and endangered species in Korea. pp 30-153. Korean Asso Conser Nature, Seoul.
8 Kim SS, Seo MH (2012) Life histories of Korean Butterflies. Sakyejul Publishing Co.
9 Kim YS, Hong SP (1990) Review of main butterflies species protected under Korean Law. Amateur Lep Soc Korea 3, 9-16.
10 Korean Amateur Lepidopterist (1986) list of butterfies from Kyeonggido, Korea. Amateur Lep Soc Korea 1, 1-19.
11 Lukhtanov VA, Sourakov A, Zakharov EV, Hebert PDN (2009) DNA barcode central Asian butterflies: increasing geographical dimension does not significantly reduce the success of species identification. Mol Eco Res 9, 1302-1310.   DOI   ScienceOn
12 Ministry of Environment (2005) Endangered plants and animals in Korea. Ministry of Environment, Seoul.
13 Park HC (2005) Endangered plants and animals: insect 4: Aporia. http://www.hani.co.kr/kisa/section-010003000/2005/12/010003000200512061709348.html.
14 Park HC, Kim SS (2001) Study on current and new proposal of endangered plants and animals in Korea. Insects. pp 91-114. Ministry Enviro Kor Asso Conser Nat, Seoul.
15 Park JS, Cho Y, Kim MJ, Nam SH, Kim I (2012) Description of complete mitochondrial genome of the black-veined white, Aporia crataegi (Lepidoptera: Papilionoidea), and comparison to papilionoid species. J Asia-Paci Ento 15, 331-341.   과학기술학회마을   DOI   ScienceOn
16 Shapiro AM, Forister ML, Fordyce JA (2007) Extreme highaltitude asian and andean Pierid butterflies are not each others' closest relatives. Arc Anta Alp Rese 39, 137-142.   DOI   ScienceOn
17 Shin YH (1990) Study on survey of rare and threatened plants and animals in Korea. Insects. Bull Kor Asso Con Nat 10, 145-169.
18 Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Bio Evo 28, 2731-2739.   DOI   ScienceOn
19 Kim HC (1992) Butterfly-fauna of Changwon-ri area in Yongwol-gun, Kangweon-do. Amateur Lep Soc Korea 5, 6-12.
20 Wahlberg N, Wheat CW (2008) Genomic outposts serve the phylogenomic pioneers: Designing novel nuclear markers for genomic DNA extractions of Lepidoptera. Sys Bio 57, 231-242.   DOI   ScienceOn
21 Paek MK, Shin YH (2010) Butterflies of the Korean peninsula. In: Cho, YK(eds) Academic Series 1, Nature & Ecology press, Seoul.
22 Korean Amateur Lepidopterist (1989) Butterfly-fauna of Kangwon-do, Korea. Amateur Lep Soc Korea 1, 1-19.