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Seasonal Change of RNA/DNA Ratio and Blood Characteristics of Black Sea Bream Acanthopagrus schlegeli Habituated in Geojae Costal Area, Kyungnam Province, Korea

경남 거제해역에 서식하는 감성돔(Acanthopagrus schlegeli)의 연간 RNA/DNA 및 혈액학적 특성 변화

  • 김수경 (국립수산과학원 서해수산연구소) ;
  • 심나영 (국립수산과학원 서해수산연구소) ;
  • 이도현 (수산자원사업공단 수산종묘실) ;
  • 김대현 (수산자원사업공단 수산종묘실) ;
  • 윤성종 (수산자원사업공단 수산종묘실)
  • Received : 2012.08.21
  • Accepted : 2012.12.28
  • Published : 2013.01.31

Abstract

The monthly variations of blood characteristics and RNA/DNA of black sea bream, Acanthopagrus schlegeli, habituated in Geojae costal area were analysed to determine health condition of natural stocks in terms of gonad maturation and spawning season from March 2010 to February 2011. Spawning season determinated by gonadosomatic index is from June to August. RNA/DNA ratio of black sea bream muscle was strongly correlated with spawning season. During the gonad maturation RNA/DNA ratio in dorsal muscle tissue was decreased contrast to rapid increase during spawning season. Blood composition factors increased in terms of gonad maturation are aspartate aminotransferase, cholesterol, triglyceride, total protein, glucose, globulin, alkaline phosphatase and inorganic phosphate. Other blood factors increased during spawning season are alanine aminotransferase, blood urea nitrogen, uric acid and lactate dehydrogenase.

Keywords

References

  1. Arukwe, A., Goksoyr, A., 2003, Eggshell and egg yolk proteins in fish, hepatic proteins for the next generation, oogenetic, population and evolutionary implications of endocrine disruption, Comp. Hepatol., 2, 1-21. https://doi.org/10.1186/1476-5926-2-1
  2. Amara, R., 2004, 0-group flatfish growth conditions on a nursery ground (Bay of Canche, Eastern English Channel), Hydrobiologia, 518, 23-32. https://doi.org/10.1023/B:HYDR.0000025053.62966.cd
  3. Araki, H., Schmid, C., 2010, Is hatchery stocking a help or harm? Evidence, limitations and future directions in ecological and genetic surveys, Aquaculture, 308, 52-511.
  4. Belchier, M., Clemmesen, C., Corted, L., Doan, T., Folkvord, A., Garcia, A., Geffen, A., Hoje, H., Johannessen, A., Moksness, E., de Pontua, H., Ramirez, R., Schnack, D., Sveinsbo, B., 2004, Recruitment studies: manual on precision and accuracy of tool, ICES Techniques in Marine Environmental Sciences, No. 33, Copenhagenn, Denmark, 1-35.
  5. Baeverfjord, G., Åsgárd, T., Shearer, K. D., 1988, Development and detection of phosphorus deficiency in Atlantic salmon, Salmo salar L., parr and post-smolts. Aquac. Nutr., 4, 1-11.
  6. Bulow, F. J., Coburn, B. Jr., Cobb, C. S., 1978, Comparisons of two bluegill populations by means of the RNA-DNA ratio and liver somatic index, Trans. Amer. Fish. Soc., 107, 799-803. https://doi.org/10.1577/1548-8659(1978)107<799:COTBPB>2.0.CO;2
  7. Chatzifotis, S., Muje, P., Pavlidis, M., Agren, J., Paalavuo, M., Molsa, H., 2004, Evolution of tissue composition and serum metabolites during gonadal development in the common dentex (Dentex dentex), Aquaculture, 236, 557-573. https://doi.org/10.1016/j.aquaculture.2003.12.004
  8. Childress, J. J., Somero, G. N., 1990, Metabolic scaling: a new perspective based on scaling of glycolytic enzyme activities, American Zoologist, 30, 161-173. https://doi.org/10.1093/icb/30.1.161
  9. Chyung, M. K, 1977, The fishes of Korea, Il Ji publishing Co., Seoul, Korea, 359-360.
  10. Claybrook, D. L., 1983, Nitrogen metabolism, in: Mantel, L. H. (ed.), The biology crustacea, Internal Anatomy and Physiological Regulation, vol. 5. Academic Press, New York, 163-213.
  11. Clemmesen, C., 1994, The effect of food availability, age or size on the RNA and DNA content in individual measured herring larvae, laboratory calibration, Mar. Biol., 118, 377-382. https://doi.org/10.1007/BF00350294
  12. Eliassen, J. E., Vahl, O., 1982, Seasonal variations in the gonad size and the protein and water content of cod, Gadus morhua (L.) muscle from Northern Norway, J. Fish. Biol., 20, 527-533. https://doi.org/10.1111/j.1095-8649.1982.tb03953.x
  13. Fushimi, H., 2001, Production of juvenile marine finfish for stock enhancement in Japan, Aquaculture, 200, 33-53. https://doi.org/10.1016/S0044-8486(01)00693-7
  14. Gershanovich, A. D., Pototskij, I. V., 1992, The peculiarities of nitrogen excretion in sturgeons (Acipenser ruthenus) (Pisces, Acipenseridae), I. The influence of ration size, Comp. Biochem. Physiol., 103A, 609-612.
  15. Gonzalez, E. B., Umino, T., Kagasawa, K., 2008, Stock enhancement programme for black sea bream, Acanthopagrus schlegelii (Bleeker), in Hiroshima Bay, Japan: a review, Aquaculture Research, 39, 1307-1315. https://doi.org/10.1111/j.1365-2109.2008.01996.x
  16. Gonzalez, E. B., Murakami, T., Yoneji, T., Nagasawa, K., Umino, T., 2009, Reduction in size-at-age of black sea bream (Acanthopagrus schlegelii) following intensive releases of cultured juveniles in Hiroshima Bay, Japan. Fish. Res., 99, 130-133. https://doi.org/10.1016/j.fishres.2009.04.013
  17. Guraya, S. S., 2000, The biology of gonadal development, sex differentiation, maturation and sex reversal in fish; cellular, molecular and endocrinological aspects, Proc. Indian Nat. Sci. Acad., (B66) 4-5, 167-194.
  18. Hardy, R. W., Shearer, K. D., 1985, Effect of dietary calcium phosphate and zinc supplementation on whole body zinc concentration of rainbow trout (Salmo gairdneri), Can. J. Aquat. Sci., 42, 181-184. https://doi.org/10.1139/f85-022
  19. Haines, T. A., 1980, Seasonal patterns of muscle RNA-DNA ratio and growth in black crappie, Pomixis nigromaculatus, Env. Biol. Fish., 5(1), 65-70.
  20. Htun-Han, M., 1978, The reproduction biology of the dab, Limanda limanda (L.) in the North Sea; gonadosomatic index, hepatosomatic index and condition factor, J. Fish. Biol., 13, 269-278.
  21. Johnston, C. E., Gray, R. W., Mclennan, A., Peterson, A., 1987, Effects of photoperiod, temperature, and diet on the reconditioning response, blood chemistry, and gonad maturation of Atlantic salmon kelts (Salmo salar) held in freshwater, Can. J. Fish. Aqu. Sci., 44, 702-711. https://doi.org/10.1139/f87-085
  22. Kikuchi, K., Takeda, S., Honda, H., and Kyono, M., 1991, Effect of feeding on nitrogen excretion of Japanese flounder, Paralichthys olivaceus, Nippon Suisan Gakkaishi, 57, 2059-2064. https://doi.org/10.2331/suisan.57.2059
  23. Kim, S. K., Rosenthal, H., Clemmesen, C., Park, K. Y., Kim, D. H., Choi, Y. S., Seo, H. C., 2005, Various methods to determine the gonadal development and spawning season of the purplish washington clam, Saxidomus purpuratus (Sowerby), J. Appl. Ichthyol., 21, 101-106. https://doi.org/10.1111/j.1439-0426.2004.00636.x
  24. Kinoshita, Y., 1939, Studies on the sexuality of genus Sparus, J. of Science of the Hiroshima University Series B Division 1. Zoology, 7, 25-37.
  25. Kitada, S., Kishano, H., 2006, Lessons learned from Japanese marine finfish stock enhancement programmes, Fish. Res., 80, 101-112. https://doi.org/10.1016/j.fishres.2006.03.019
  26. Mahboob, S., Sheri, A. N., 1997, Relationships among ovary weight, liver weight and body weight in female grass carp C. idella, J. Aqua. Trop., 12, 255-259.
  27. Mustafa, S., 1977, Influence of maturation on the concentrations of RNA and DNA in the flesh of the catfish Clarias batrachus, Trans. Amer. Fish. Soc., 106, 449-451. https://doi.org/10.1577/1548-8659(1977)106<449:IOMOTC>2.0.CO;2
  28. Naghama, Y., 1983, The functional morphology of teleost gonads; In: (eds.) Fish physiology Reproduction Hoar. W. S., Randall, J. and E. M. Donaldson Academic press, New York, 223-275.
  29. Ng, T. B., Idler, T. B., 1983, Yolk formation and differentiation in teleost fishes, in: Hoar W. S., Randall D. J. and Donaldson E. M. (eds.), Fish Physiology, vol 9. Part B, Academic Press, New York London, 373-404.
  30. Ng, T. B., Tam, P. P., Woo, N. Y., 1988, Sexual maturation in the black sea bream, Mylio macrocephalus teleostei, Sparidae: changes in pituitary gonadotropes, hepatocytes and related biochemical constituents in liver and serum, Cell Tissue Res., 245(1), 207-213.
  31. Phelan, B. A., Goldberg, R., Bejda, A. J., Pereira, J., Hagan, S., Clark, P., Studholme, A. L., Calabrese, A., Able, K. W., 2000, Estuarine and habitat-related differences in growth rates of young-of-the year winter flounder (Pseudopleuronectes americanus) and tautog (Tautoga onitis) in three northeastern US estuaries, J. of Exp. Mar. Biol. and Ecol., 247, 1-28. https://doi.org/10.1016/S0022-0981(99)00184-7
  32. Regnault, M., 1987, Nitrogen excretion in marine and freshwater crustacean, Biol. Rev., 62, 1-24. https://doi.org/10.1111/j.1469-185X.1987.tb00623.x
  33. Satomi, Y., Ishida, R., 1976, Seasonal changes of nucleic acid contents in liver, and red and ordinary muscle of Ayu-fish (Plecoglossus altivelis T. and S.) reared in ponds, Bull. Freshw. Fish. Lab.(Tokyo), 26, 35-44.
  34. Schoenebeck, C. W., Brown, M. L., 2012, Does anaerobic activity differ seasonally or between sexes in yellow perch population?, Transactions of the American Fisheries Society, 141, 199-203. https://doi.org/10.1080/00028487.2012.655119
  35. Selch, T. M., Chipps, S. R., 2007, The cost of capturing prey: measuring largemouth bass (Micropterus salmoides) foraging activity using glycolytic enzyme (lactate dehydrogenase), Can. J. of Fish Aquat. Sci. 64, 1761-1769. https://doi.org/10.1139/f07-133
  36. Tatrai, I., 1981, Diurnal pattern of the ammonia and urea excretion of feeding and starved bream, Abramis brama L, Comp. Biochem. Physiol., 70A, 211-215.
  37. Zaboukas, N., Miliou, H., Megalofonou, P., Moraitou- Apostolopoulou, M., 2006, Biochemical composition of the Atlantic bonito Sarda sarda from the Aegean Sea (eastern Mediterranean Sea) in different stages of sexual maturity, J. of Fish. Biol., 69, 347-362. https://doi.org/10.1111/j.1095-8649.2006.01090.x