한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제28권4호
  • /
  • Pages.367-375
  • /
  • 2013
  • /
  • 1229-1153(pISSN)
  • /
  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
권호 표지
DOI QR코드

DOI QR Code

해동피 및 유사생약 추출물의 위 손상에 대한 효과

Effects of Kalopanax Pictus Extracts and Their Related Origin on Gastric Lesions

  • 황인영 (덕성여자대학교 약학대학) ;
  • 황선아 (덕성여자대학교 약학대학) ;
  • 정춘식 (덕성여자대학교 약학대학)
  • 투고 : 2013.08.13
  • 심사 : 2013.12.18
  • 발행 : 2013.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

해동피는 약리학적으로 진통, 소염작용이 있어, 허리와 무릎의 통증 등을 치료하는데 효과가 있다고 알려져 있다. 본 연구에서는 해동피(Kalopanax pictus)의 국산 및 중국산과 그 유사생약으로 사용되는 절동피(znthoxylum ailanthoides)의 국산과 중국산, 목면피(bombax malabaricum)의 $H_2O$ 및 70% EtOH 추출물의 항위염 효과와 항산화효과에 대해 알아보았다. 먼저 HCl EtOH로 유발한 흰쥐위염 모델을 통해 효과를 확인하였고, 위염 및 위궤양, 위암의 원인으로 잘 알려진 H. pylori에 대한 colony 억제작용과 대표적 공격인자인 과다한 산에 대한 억제작용을 확인 하고자 제산력 시험법을 실시하였다. 항위염 효과에 대한 항산화 기전으로는 DPPH 시험법, 환원력 시험법, ${\beta}$-carotene/linoleic acid syetem을 이용한 지질과산화 억제활성 시험을 실시하였고, 실험에 사용한 추출물 중 가장 효과가 우수한 해동피 국산 추출물을 이용하여 superoxide radical 및 hydrogen peroxide 소거 작용을 확인하였다. Indomethacin으로 유발한 흰쥐 위궤양 모델에서는 해동피 국산 $H_2O$ 및 70% EtOH 추출물에서 각각 73.1%, 53.5%로 손상을 억제시킴을 확인하였다. 흰쥐를 이용한 gastric secretion 실험으로는 해동피 국산 $H_2O$ 및 70% EtOH 추출물이 위액의 분비를 줄이고, pH를 상승 시켰으며 총 산도를 감소시킴을 확인하였다. 그 결과, 해동피국산 및 중국산, 절동피국산 및 중국산, 목면피중국산 해동피의 항산화 효과가 가장 우수하였으며 해동피 국산은 위염 및 위궤양에서 우수한 효과를 확인하였고, 건강 기능성 식품 및 천연물 의약품으로서의 개발 가능성을 확인할 수 있었다. 또한, 이 결과를 토대로 오랜 세월 한약재로 사용되어 온 해동피의 기원이 다른 유사품 및 불량약재의 무분별한 유통, 정확한 기원의 약재에 대한 기준 설정, 높아지는 한약재 수입 의존도에 따른 위품, 저질품의 혼입 방지와 객관적, 신뢰성 있는 표준규격 설정에 기여할 것으로 예상된다.

Kalopanax pictus has pharmacologically anti-inflammatory and analgesic effect and is known to respond to treatment of backache, knee pain and etc. In this study, we investigated the effects on gastric lesions of Kalopanax pictus both from Korea (KPK) and China (KPC) compared with their related origin, Znthoxylum ailanthoide both from Korea (ZAK) and China (ZAC), and Korean Bombax malabaricum (BMK). In preliminary screening, KPK and KPC shown effective inhibition of HCI EtOH-induced gastritis in rats. To elucidate their protective effects on gastric lesions, we assessed inhibition of H. pylori colonization, 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activities, reducing power test, and inhibition of lipid peroxidation. KPK was the most effective from antioxidant assays. KPK also shown the inhibition of indomethacin-induced gastric ulcer in rats. Gastric secretion in rats, KPK reduced the secretion of gastric juice and total acidity and raised pH. Therefore, it is possible that KPK can be developed as health functional food and natural medicine. In addition, it can contribute to the standardization with objectivity and reliability for KPK through the criteria establishment of the precise origin of medicine, the prevention of indiscriminate distribution of imitation, and the rising rate of dependence on imports of medicinal herbs, and mixing prevention of low-quality goods.

키워드

참고문헌

  1. 생약학교재편찬위원회: 생약학. 동명사, 경기, pp. 549 (2006).
  2. Massimo, R. and Robert, M.G.: Staging and grading of chronic gastritis. Human Pathol., 36, 228-233 (2005). https://doi.org/10.1016/j.humpath.2004.12.008
  3. Long, L.H., Evans, P.J. and Halliwell, B.: Hydrogen peroxide in human urine: implications for antioxidant defense and redox regulation. Biochem. Biophys. Res. Commun., 263, 605-609 (1999).
  4. Nishimiki, M., Rao, N.A., Appaji, N. and Yagi, K.: The occurrence of superoxide anion in the reaction of reduced henazine methosulfate and molecular oxygen. Biochem. Biophys. Res. Commun., 46, 849-854 (1972). https://doi.org/10.1016/S0006-291X(72)80218-3
  5. Subhashinee, S., Wijeratne, K., Susan, L. and Vicky, S.: Hydrogen peroxide induced oxidative stress damage and antioxidant enzyme response in caco-2 human colon cells. J. Agric. Food Chem., 53, 8768-8774 (2005). https://doi.org/10.1021/jf0512003
  6. Warren, J.S., Kunkel, S.L., Cunningham, T.W., Johnson, K.J. and Ward, P.A.: Macrophage-derived cytokines amplify immune complex-triggered $O^{2-}$. responses by rat alveolar macrophages. Am. J. Pathol., 130, 489-495 (1988).
  7. Yoshikawa, T., Takemura, T., Seto, O., Tanigawa, T., Tainaka, K., Morita, Y., Yoshida, N., Sugino, S. and Kondo, M.: Role of lipid peroxidation and polymorphonuclear leukocytes-derived oxygen radicals in acute gastric lesions induced by hyperthermic treatment. Nihon Shokakibyo Gakkai Zasshi, 86, 159-164 (1989).
  8. Itoh, M. and Guth, P.H.: Role of oxygen-derived free radicals in hemorrhagic shock-induced gastric lesions in the rat. Gastroenterology, 88, 1162-1167 (1985). https://doi.org/10.1016/S0016-5085(85)80075-5
  9. Mizui, T. and Dodeuchi, M.: Effect of polyamines on acidified EtOH induced gastric lesion in rats. Jpn. J. Pharmacol., 33, 939-945 (1983). https://doi.org/10.1254/jjp.33.939
  10. Bae, E.A., Han, M.J., Baek, N.I. and Kim, D.H.: In vitro anti Helicobacter pylori activity of panaxtriol isolated from ginseng. Arch. Pharm. Res., 24, 297-299 (2001). https://doi.org/10.1007/BF02975095
  11. Kim, J.M., Shin, J.E., Han, M.J., Baek, N.I. and Kim, D.H.: Inhibitory effect of ginseng polyacetylenes on infection and vacuolation of Helicobacter pylori. Natural Product Sciences, 9, 158-160 (2003).
  12. Uchiyama, M., Suzuki, Y. and Fukuzawa, K.: Biochemical studies of physiological function of tocopherolactone. Yakugaku Zasshi, 88, 678-683 (1968).
  13. Oyaizu, M.: Studied on products of browning reaction; antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr., 44, 307-315 (1986). https://doi.org/10.5264/eiyogakuzashi.44.307
  14. Koleva, I.I., Beek, T.A., Linssen, J.P.H., Groot, A. and Evastatieva, L.N.: Screening of plant extracts for antioxidant activity; a comparative study on three testing methods. Phytochem. Anal., 13, 8-17 (2002). https://doi.org/10.1002/pca.611
  15. Nishimiki, M. Rao, N.A., Appaji, N. and Yagi, K.: The occurrence of superoxide anion in the reaction of reduced henazine methosulfate and molecular oxygen. Biochem. Biophys. Res. Commun., 46, 849-854 (1972). https://doi.org/10.1016/S0006-291X(72)80218-3
  16. Kasuya, Y., Urushidani, T. and Okabe, S.: Effects of various drugs and vagotomy on indomethacin-induced gastric ulcers in the rat. Jpn. J. Pharmacol., 29, 670-673 (1979). https://doi.org/10.1254/jjp.29.670
  17. Choi, C.H., Cha, Y.J., An, C.S., Kim, K.J., Kim, K.C., Moon, S.P., Lee, Z.H. and Min, Y.D.: Molecular mechanisms of heptaplatin effective against cisplatin-resistant cancer cell lines: less involvement of metallothionein. Cancer Cell Int., 4, 6-17 (2004). https://doi.org/10.1186/1475-2867-4-6
  18. Shay, H. and Komarov, S.A., Fels, S.S. and Meranze, D.: A simple method for the uniform production of gastric ulceration in the rat. Gastroenterol., 4, 43-61 (1945).
  19. Behera, B.C., Adawadkar, B. and Makhija, U.: Inhibitory activity of xanthine oxidase and superoxide-scavenging activity in some taxa of the lichen family Graphidaceae. Phytomed., 10, 536-543 (2003). https://doi.org/10.1078/094471103322331511
  20. Lee, E.B., Li, D.W., Hyun, J.E., Kim, I.H. and Whang, W.K.: Anti-inflammatory activity of methanol extract of Kalopanax pictus bark and its fractions. J. Ethnopharmacol., 77, 197-201 (2001). https://doi.org/10.1016/S0378-8741(01)00301-4
  21. Choi, L., Han, Y.N., Lee, K.T., Park, K.Y., Kwak, T.S., Kwon, S.H. and Park, H.L.: Anti-lipid peroxidative principles from the stem bark of Kalopanax pictus Nakai. Arch. Pharm. Res., 24, 536-540 (2001). https://doi.org/10.1007/BF02975161
  22. Choi, J., Huh, K., Kim, S.H., Lee, K.T., Park, H.J. and Han, Y.N.: Antinociceptive andanti-rheumatoidal effects of Kalopanax pictus extract and its saponin components in experimental animals. J. Ethnopharmacol., 79, 199-204 (2002). https://doi.org/10.1016/S0378-8741(01)00383-X
  23. Hu, W., Lee, S.K., Jung, M.J., Heo, S.I., Hur, J.H. and Wang, M.H.: Induction of cell cycle arrest and apoptosis by the ethyl acetate fraction of Kalopanax pictus leaves in human colon cancer cells. Bioresour. Technol., 101, 9366-9372 (2010). https://doi.org/10.1016/j.biortech.2010.06.091
  24. Bang, S.Y., Park, G.Y., Park, S.Y., Kim, J.H., Lee, Y.K., Lee, S.J. and Kim, Y.: The stem bark of Kalopanax pictus exhibits anti-inflammatory effect through heme oxygenase-1 induction and NF-${\kappa}B$ suppression. Immune Netw., 10, 212-218 (2010). https://doi.org/10.4110/in.2010.10.6.212

피인용 문헌

  1. Comparison of Phenolic Acid from Shoots of Aralia elata and Kalopanax pictus Cultivated in Korea Using UPLC-DAD-ESI(+)-QToF/MS vol.37, pp.4, 2018, https://doi.org/10.5338/KJEA.2018.37.4.37