Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Effects of Light Quality Using LEDs on Expression Patterns in Brassica rapa Seedlings

LED 광원의 다양한 광질이 배추 유묘의 유전자 발현에 미치는 영향

  • Kim, Jin A (Functional Biomaterial Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Yeon-Hee (Functional Biomaterial Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Hong, Joon Ki (Functional Biomaterial Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Hong, Sung-Chang (Climate Change & Agroecology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Soo In (Functional Biomaterial Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Choi, Su Gil (Functional Biomaterial Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Moon, Yi-Seul (Functional Biomaterial Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Koo, Bon-Sung (Functional Biomaterial Division, National Academy of Agricultural Science, Rural Development Administration)
  • 김진아 (농촌진흥청 국립농업과학원 생물소재공학과) ;
  • 이연희 (농촌진흥청 국립농업과학원 생물소재공학과) ;
  • 홍준기 (농촌진흥청 국립농업과학원 생물소재공학과) ;
  • 홍성창 (농촌진흥청 국립농업과학원 기후변화생태과) ;
  • 이수인 (농촌진흥청 국립농업과학원 생물소재공학과) ;
  • 최수길 (농촌진흥청 국립농업과학원 생물소재공학과) ;
  • 문이슬 (농촌진흥청 국립농업과학원 생물소재공학과) ;
  • 구본성 (농촌진흥청 국립농업과학원 생물소재공학과)
  • Received : 2012.09.20
  • Accepted : 2013.05.06
  • Published : 2013.09.30
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Abstract

Light with two faces, beneficial and harmful effects is an important signal for every living cell. Optimal adaptation to light environment enhances the fitness of an organism and survival in nature. Understandings of light quality and plant growth provide with the economical guides for artificial light sources like LEDs. Compared with those under white light, the 1 week seedlings of Chinese cabbage (Brassica rapa) under monochromic red and blue light showed normal development and growth. In contrast to extremely long and etiolated hypocotyls of the seedlings under dark, those under far-red etiolated were extremely short. Based on the microarray analysis, blue light induced the vigorous development and growth and two fold changes of transcripts than red light condition. To have insight of gene products under different light qualities conditions, GO term enrichments were calculated and each gene according to their GO terms were categorized. The blue and red lights affected the expressions of genes related to biological process. Especially, the genes related to metabolic process and developmental process and plastid and chloroplast in the cellular component category were induced under blue light. This study provided the molecular biological evidence for various light qualities on the growing process of B. rapa.

광은 모든 살아 있는 세포에 있어서 매우 중요한 신호이며 생물체에게 이익이 되면서 동시에 해가 되기도 하는데, 광 환경에 잘 적응한 유기체는 자연에서의 경쟁에서 더 잘 살아남을 수 있다. 광질과 식물 생육에 대한 정보는 LED와 같은 인공광을 효율적으로 사용하게 하여 경제적인 손실을 줄일 수 있다. 초기 1주 동안 단일 광파장에 노출된 배추 유묘의 생장을 보면 백색광 조건에서 생장한 묘와 비교했을 때 적색광과 청색광 조건에서는 유묘의 발달과 생육이 정상이었다. 암조건에서 자란 유묘는 하배축이 길고 황화된 반면, 초적외광 조건에서 자란 유묘는 황화되었으나 하배축이 거의 신장하지 않았다. 마이크로어레이를 이용하여 유전자 대량 발현분석 실험을 수행한 결과, 청색광 조건에서 유묘의 생육이 가장 왕성하였으며 적색광 조건과 비교하여 발현변화를 보인 유전자의 수가 2배 많았다. 각각의 광질 조건에서 발현 증가 된 유전자들을 기능별로 분류해 보면(GO 분석) 적색광과 청색광은 생물학적 과정(biological process)과 관련된 유전자의 발현에 영향을 많이 주었다. 특히 대사과정(metabolic process)과 발달과정(developmental process) 그리고 세포 요소(cellular component) 중 색소체(plastid)나 엽록체(chloroplast)관련 유전자들의 발현이 청색광 조건에서 더 많이 증가하였다. 또한 초적외광 조건에서도 다양한 유전자들이 발현 변화를 보였다. 본 논문은 다양한 배추 유묘의 생장에 대한 광질의 영향을 분자생물학적으로 증명하기 위해 수행되었으며 유묘의 배양에 LED 광원이 효율적으로 이용될 수 있음을 보여 주었다.

Keywords

References

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