Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Molecular Diagnosis of Grapholita molesta and Grapholita dimorpha and Their Different Occurrence in Peach and Plum

복숭아순나방과 복숭아순나방붙이의 분자동정법 개발 및 복숭아와 자두에서의 발생차이

  • Ahn, Seung-Joon (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Choi, Kyung-Hee (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kang, Taek Jun (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Hyung Hwan (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Dong-Hwan (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Cho, Myoung Rae (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Yang, Chang Yeol (National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 안승준 (농촌진흥청 국립원예특작과학원) ;
  • 최경희 (농촌진흥청 국립원예특작과학원) ;
  • 강택준 (농촌진흥청 국립원예특작과학원) ;
  • 김형환 (농촌진흥청 국립원예특작과학원) ;
  • 김동환 (농촌진흥청 국립원예특작과학원) ;
  • 조명래 (농촌진흥청 국립원예특작과학원) ;
  • 양창열 (농촌진흥청 국립원예특작과학원)
  • Received : 2013.10.22
  • Accepted : 2013.11.06
  • Published : 2013.12.01
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Abstract

The plume fruit moth, Grapholita dimorpha Komai, a fruit tree pest occurring in the northeast Asia, was firstly reported to infest apple in Korea in 2009, but its direct damage to other fruit trees has been poorly studied. In this study, we investigated shoots and fruits of both peach and plum trees and compared their damage rates by G. dimorpha to those by G. molesta, a congeneric species. In order to discriminate the two moth species, we developed a molecular diagnosis method using species-specific primer sets on different PCR conditions and distinguished the two species collected from the damaged shoots or fruits. The shoots and fruits of peach were infested mostly by G. molesta. However, in plums, the shoots were damaged by G. molesta and the fruits mostly by G. dimorpha. In addition, these two species showed a clear difference in host preference in fruit damage, where 92.5% of the Grapholita moths collected in peach fruits were identified as G. molesta, but 97.0% of the moths in plum fruits were G. dimorpha. The difference of the damage between the two fruit trees may give important information for monitoring of the two moth species in these orchards.

복숭아순나방붙이(Grapholita dimorpha Komai)는 동북아시아에 주로 발생하는 과수 해충으로, 국내에서는 2009년에 사과에 피해를 준다는 것이 보고되었으나, 그 이외의 과수에서는 직접적인 피해가 명확히 알려지지 않았다. 본 연구는 핵과류인 복숭아와 자두를 대상으로 복숭아 순나방류의 피해로 보이는 피해순과 피해과를 채집하여, 복숭아순나방붙이와 그 유사종인 복숭아순나방(Grapholita molesta Busck)의 피해율을 비교 조사하였다. 두 유사종의 정확한 구별을 위해 우선 종 특이적 프라이머와 PCR 반응조건을 이용한 분자동정법을 개발하였다. 복숭아와 자두의 신초와 과실을 가해하는 종을 야외에서 채집하여 분자동정법으로 확인한 결과, 복숭아의 신초와 과실은 거의 모두 복숭아순나방이 가해하였으며, 자두는 신초의 경우 복숭아순나방이, 과실의 경우 복숭아순나방붙이가 주로 가해하는 것으로 나타났다. 즉, 복숭아와 자두에서 조사한 신초는 모두(100%) 복숭아순나방에 의해 피해를 받았으나, 복숭아 과실은 대부분(92.5%) 복숭아순나방이, 자두 과실은 대부분(97.0%) 복숭아순나방붙이가 가해하는 것으로 나타났다. 본 연구결과는 복숭아순나방붙이가 복숭아 보다는 자두 과실에 주로 피해를 준다는 것을 보여주며, 특히 자두나무에서도 신초와 과실에 따라 각기 다른 종이 피해를 준다는 점을 보여준다. 이렇게, 기주식물에 따라 두 유사종의 가해특성이 다른 것은 복숭아와 자두과원에서 두 종의 발생을 예찰하는데 중요한 정보를 제공할 것으로 여겨진다.

Keywords

References

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  2. Larval species composition and genetic structures of Carposina sasakii, Grapholita dimorpha, and Grapholita molesta from Korea 2017, https://doi.org/10.1017/S0007485317000694
  3. Simultaneous identification ofGrapholita molestaBusck andGrapholita dimorphaKomai by PCR-RFLP vol.46, pp.3, 2016, https://doi.org/10.1111/1748-5967.12166
  4. Distribution Ratios of Grapholita molesta and G. dimorpha Larvae in Pest-damaged Fruits of Five Rosaceae Fruit Trees 2016, https://doi.org/10.5656/KSAE.2016.07.0.038