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http://dx.doi.org/10.5656/KSAE.2013.11.0.072

Molecular Diagnosis of Grapholita molesta and Grapholita dimorpha and Their Different Occurrence in Peach and Plum  

Ahn, Seung-Joon (National Institute of Horticultural and Herbal Science, Rural Development Administration)
Choi, Kyung-Hee (National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kang, Taek Jun (National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Hyung Hwan (National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Dong-Hwan (National Institute of Horticultural and Herbal Science, Rural Development Administration)
Cho, Myoung Rae (National Institute of Horticultural and Herbal Science, Rural Development Administration)
Yang, Chang Yeol (National Institute of Horticultural and Herbal Science, Rural Development Administration)
Publication Information
Korean journal of applied entomology / v.52, no.4, 2013 , pp. 365-370 More about this Journal
Abstract
The plume fruit moth, Grapholita dimorpha Komai, a fruit tree pest occurring in the northeast Asia, was firstly reported to infest apple in Korea in 2009, but its direct damage to other fruit trees has been poorly studied. In this study, we investigated shoots and fruits of both peach and plum trees and compared their damage rates by G. dimorpha to those by G. molesta, a congeneric species. In order to discriminate the two moth species, we developed a molecular diagnosis method using species-specific primer sets on different PCR conditions and distinguished the two species collected from the damaged shoots or fruits. The shoots and fruits of peach were infested mostly by G. molesta. However, in plums, the shoots were damaged by G. molesta and the fruits mostly by G. dimorpha. In addition, these two species showed a clear difference in host preference in fruit damage, where 92.5% of the Grapholita moths collected in peach fruits were identified as G. molesta, but 97.0% of the moths in plum fruits were G. dimorpha. The difference of the damage between the two fruit trees may give important information for monitoring of the two moth species in these orchards.
Keywords
Grapholita molesta; Grapholita dimorpha; Peach; Plum; Molecular diagnosis;
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Times Cited By KSCI : 6  (Citation Analysis)
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