생명과학회지 (Journal of Life Science)

  • 제22권10호
  • /
  • Pages.1339-1343
  • /
  • 2012
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

Platycodin D를 포함하는 도라지 추출물이 DNCB 유도 알레르기성 아토피 피부염에 미치는 영향

Inhibitory Effect of PG-Platycodin D on the Development of Atopic Dermatitis-Like Skin Lesions in ICR Mice

  • 박세진 (연세대학교 과학기술대학 생명과학기술학부) ;
  • 김윤석 (연세대학교 보건과학대학 임상병리학과) ;
  • 김택중 (연세대학교 과학기술대학 생명과학기술학부)
  • Park, Sae-Jin (Division of Biological Science and Technology, College of Science and Technology, Yonsei University) ;
  • Kim, Yoon Suk (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Kim, Tack-Joong (Division of Biological Science and Technology, College of Science and Technology, Yonsei University)
  • 투고 : 2012.07.20
  • 심사 : 2012.10.22
  • 발행 : 2012.10.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

알레르기성 아토피 피부염은 면역적 반응을 포함하는 복잡한 원인을 가진 염증성 질환이다. 본 연구는 ICR mice의 등쪽 부위에 반복적인 DNCB 도포를 통해 유발시킨 알레르기성 아토피 피부염에 있어서 PG-Platycodin D의 효능에 대해 연구하였다. PG-Platycodin D의 효능은 scratching behavior, skin severity score, 그리고 조직병리학적 관찰을 통해서 확인하였다. PG-Platycodin D가 scratching behavior, skin severity score 수준을 감소시킴을 확인할 수 있었다. 또한, 조직병리학적 관찰에 의해 PG-Platycodin D군에서 피부의 부종이 효과적으로 억제됨을 알 수 있었다. 이러한 결과로부터 알레르기성 아토피 피부염에 있어서 Platycodin D가 포함된 도라지 추출물이 유용한 천연 원료 물질로서 가능성을 가짐을 확인하였다.

Atopic dermatitis is characterized by chronically pruritic and inflammatory dermatitis. In this study, we investigated the effect of Platycodon grandiflorum including platycodin D (PG-Platycodin D) in an atopic dermatitis-like mouse model. An atopic dermatitis-like skin lesion was induced by repeated treatment of 2,4-dinitrochlorobenzene (DNCB) on the dorsal skin of ICR mice. The efficacy of PG-Platycodin D was tested by observing scratching behavior, the skin severity score, and histopathologic analysis. PG-Platycodin D reduced the DNCB-induced increase in scratching behavior and the skin severity score. In addition, histopathologic analysis revealed a reduction in the thickening of the epidermis in the PG-Platycodin D group. These results may contribute to the development of a therapeutic drug for the treatment of atopic dermatitis.

키워드

참고문헌

  1. Chio, K. U. and Paek, D. M. 1995. Asthma and air pollution in Korea. Kor. J. Epidemiol. 17, 64-75.
  2. Chung, J. H., Shin, P. G. and Cho, S. H. 1997. Chemical compositions of platycodon grandiflorus. J. Kor. Agric. Chem. Biotech. 40, 148-151.
  3. Chung, J. H., Shin, P. G. and Cho, S. H. 1997. Pharmaceutical substances of platycodon grandiflorus. J. Kor. Agric. Chem. Biotech. 40, 152-156.
  4. Dal-Pizzol, F., Klamt, F., Vianna, M., Schroder, N., Quevedo, J., Benfato, M. S., Moreira, J. C. and Walz, R. 2000. Lipid peroxidation in hippocampus early and late after status epilecticus induced by pilocarpine or kainic acid in Wistar rats. Neurosci. Lett. 291, 179-182.
  5. Gombert, M., Dieu-Nosjean, M. C., Winterberg, F., Bunemann, E., Kubitza, R.C., Da, Cunha, L., Haahtela, A., Lehtimaki, S., Muller, A., Rieker, J., Meller, S., Pivarcsi, A., Koreck, A., Fridman, W. H., Zentgraf, H. W., Pavenstadt, H., Amara, A., Caux, C., Kemeny, L., Alenius, H., Lauerma, A., Ruzicka, T., Zlotnik, A. and Homey, B. 2005. CCL1-CCR8 interactions: an axis mediating the recruitment of T cells and Langerhans-type dendritic cells to sites of atopic skin inflammation. J. Immunol. 174, 5082-5091. https://doi.org/10.4049/jimmunol.174.8.5082
  6. Ishizaka, K. 1984. Regulation of IgE synthesis. Ann. Rev. Immunol. 2, 159-182. https://doi.org/10.1146/annurev.iy.02.040184.001111
  7. Kaburagi, Y., Shimada, Y., Nagaoka, T., Hasegawa, M., Takehara, K. and Sato, S. 2001. Enhanced production of CC-chemokines (RANTES, MCP-1, MIP-1alpha, MIP-1beta, and eotaxin) in patients with atopic dermatitis. Arch. Dermatol. Res. 293, 350-355. https://doi.org/10.1007/s004030100230
  8. Kang, S. Y., Hue, S. H. and Kim, S. I. 1978. Immunologic aspects of hypersensitivity disease in Korea. Seoul Medicine 19, 12.
  9. Kim, C. H. and Chung, M. H. 1975. Pharmaceutical studies on Codonopsis lanceolate. Kor. J. Pharmacog. 6, 43-47.
  10. Kim, H. D. and Lee, J. W. 1972. Analysis of components of saponin from Ginseng, platycodon and Japanese touchwood. Kor. J. Pharmacog. 3, 51-54.
  11. Kim, M. H., Kim, M. C., Park, J. S., Kim, J. W. and Lee, J. O. 2001. The antioxidative effects of the water-soluble extracts of plants used as tea materials. Kor. J. Food Sci. Technol. 33, 12.
  12. Kim, S. Y., Kim, H. S. and Chung, S. Y. 1993. Effect of the feeding Platycodon grandiflotum and codonopsis lanceolata on the lipid components of serum and liver in rats. J. Kor. Soc. Food Nutr. 22, 524-530.
  13. Klamt, F., Dal-Pizzol, F., Frota, M. L., Walz, R., Andrades, M. E., Silva, E. G., Brentani, R., Izquierdo, I. and Moreira, J. C. 2001. Imbalance of antioxidant defence in mice lacking cellular prion protein. Free Radic. Biol. Med. 30, 1137-1144. https://doi.org/10.1016/S0891-5849(01)00512-3
  14. Lee, E. B. 1974. Phamacological studies on platycodi radix. Kor. J. Pharmacog. 5, 49-56.
  15. Leung, D. Y. M. 1995. Atopic dermatitis: the skin as a window into the pathogenesis of chronic allergic disease. J. Allergy Clin. Immunol. 96, 312-319.
  16. Matsuda, H., Watanabe, N., Geba, G. P., Sperl, J., Tsudzuki, M., Hiroi, J., Matsumoto, M., Ushio, H., Saito, S., Askenase, P. W. and Ra, C. 1997. Development of atopic dermatitis-like skin lesion with IgE hyperproduction in NC/Nga mice. Int. Immunol. 9, 461-466. https://doi.org/10.1093/intimm/9.3.461
  17. Neis, M. M., Peters, B., Dreuw, A., Wenzel, J., Bieber, T., Mauch, C., Krieg, T., Stanzel, S., Heinrich, P. C., Merk, H. F., Bosio, A., Baron, J. M. and Hermanns, H. M. 2006. Enhanced expression levels of IL-31 correlate with IL-4 and IL-13 in atopic and allergic contact dermatitis. J. Allergy Clin. Immunol. 118, 930-937. https://doi.org/10.1016/j.jaci.2006.07.015
  18. Park, U. Y., Chang, D. S. and Cho, H. R. 1992. Screening of antimicrobial activity for medicinal herb extracts. J. Kor. Soc. Food Nutr. 21, 91-96.
  19. Sator, P. G., Schmidt, J. B. and Honigsmann, H. 2003. Comparison of epidermal hydration and skin surface lipids in healthy individuals and in patients with atopic dermatitis. J. Am. Acad. Dermatol. 48, 352-358. https://doi.org/10.1067/mjd.2003.105
  20. Tasaka, K. 1986. Antiallergic drug. Drugs Today 22, 101.
  21. Vestergaard, C., Yoneyama, H., Murai, M., Nakamura, K., Tamaki, K., Terashima, Y., Imai, T., Yoshie, O., Irimura, T., Mizutani, H. and Matsushima, K. 1999. Overproduction of Th2-specific chemokines in NC/Nga mice exhibiting atopic dermatitis like lesions. J. Clin. Invest. 104, 1097-1105. https://doi.org/10.1172/JCI7613
  22. Wierenga, E. A., Snoek, M., Jansen, H. M., Bos, J. D., van, Lier, R. A. and Kapsenberg, M. L. 1991. Human atopen-specific types 1 and 2 T helper cell clones. J. Immunol. 147, 2942-2949.
  23. Yamamoto, M., Haruna, T., Yasui, K., Takahashi, H., Iduhara, M., Takaki, S., Deguchi, M. and Arimura, A. 2007. A novel atopic dermatitis model induced by topical application with dermatophagoides farinae extract in NC/Nga mice. Allergol. Int. 56, 139-148. https://doi.org/10.2332/allergolint.O-06-458

피인용 문헌

  1. Effects of Horse oil on the DNCB-induced Contact Hypersensitivity in Balb/c Mice vol.28, pp.4, 2013, https://doi.org/10.6116/kjh.2013.28.4.77
  2. on degranulation and inflammatory mediator release in RBL-2H3 cells vol.51, pp.3, 2018, https://doi.org/10.4163/jnh.2018.51.3.208