Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Anti-inflammatory Effects of Water Extract of Eucommia ulmoides OLIVER on the LPS-induced RAW 264.7 Cells

대식세포 Raw 264.7에서 두충의 항염증효과

  • Kim, Young-Ock (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Sang-Won (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Sohn, Sang-Hyun (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Seung-Yu (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Oh, Myung-Sook (Department of Oriental Pharmaceutical Science, College of Pharmacy Kyung Hee University) ;
  • Kim, Su-Kang (Kohwang Medical Research Institute, College of Medicine, Kyung Hee University)
  • 김영옥 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이상원 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 손상현 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김승유 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 오명숙 (경희대학교 약학대학 한약학과) ;
  • 김수강 (경희대학교 의과대학 고항의학연구소)
  • Received : 2012.04.07
  • Accepted : 2012.10.05
  • Published : 2012.10.30
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Abstract

Eucommia ulmoides OLIVER (EU) is a traditional Korean herbal used for the treatment of rheumatoid arthritis (RA). In the present study, the molecular pharmacology basis of its anti-inflammatory effect is revealed in this work, EU was studied in lipopolysaccharide (LPS)-activated macrophage cells (RAW 264.7) as an established inflammation model. After activation, nitric oxide (NO) production and iNOS mRNA were measured by using a colorimetric assay (Griess reagent), and reverse transcription polymerase chain reaction (RT-PCR), respectively. The change in the content of $PGE_2$, $TNF{\alpha}$, and IL-6 was concurrently monitored by ELISA. In results, we found that in the concentration range without showing cytotoxicity, EU produced a remarkable anti-inflammatory effect and showed a dose-dependent inhibition of LPSinduced NO production. Compared with indomethacin, EU has more potency and a specific action of NO inhibition, $PGE_2$, IL-6, and TNF-${\alpha}$ inhibition. These results suggest that EU may be a suitable herbal medicine to yield the greatest anti-inflammatory activity for food additives and medicine.

Keywords

References

  1. Bak JP, Son JH, Kim YM, Jung JH, Leem KH, Lee EY and Kim EH. (2011). Suppression of the inflammatory response in macrophages by Paeoniae Radix Rubra. Korean Journal of Medicinal Crop Science. 19:373-379. https://doi.org/10.7783/KJMCS.2011.19.5.373
  2. Bowen WP and Jerman JC. (1995). Nonlinear-regression using spreadsheets. Trends in Pharmacological Sciences. 16:413-417. https://doi.org/10.1016/S0165-6147(00)89091-4
  3. Diehl S and Rincon M. (2002). The two faces of IL-6 on Th1/Th2 differentiation. Molecular Immunology. 39:531-536. https://doi.org/10.1016/S0161-5890(02)00210-9
  4. Deyama T, Nishibe S and Nakazawa Y. (2001). Constituents and pharmacological effects of Eucommia and Siberian ginseng. Acta pharmacologica Sinica. 22:1057-1070.
  5. Greenway F, Lie Z, Yu Y and Gupta A. (2011). A clinical trial testing the safety and efficacy of a standardized Eucommia ulmoides Oliver bark extract to treat hypertension. Alternative Medicine Review. 16:336-347.
  6. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS and Tannenbaum SR. (1982). Analysis of nitrate, nitrite, and $[^{15}N]$ nitrate in biological fluids. Analytical Biochemistry. 126:131-138. https://doi.org/10.1016/0003-2697(82)90118-X
  7. Gu J, Wang JJ, Y J, Cui CF, Wu WH, Li L, Wang ZS, Yu M, Gao N, Liu L and Ouyang DS. (2011). Effects of lignans extracted from Eucommia ulmoides and aldose reductase inhibitor epalrestat on hypertensive vascular remodeling. Journal of Ethnopharmacology. 133:6-13. https://doi.org/10.1016/j.jep.2010.08.055
  8. Hesslinger C, Strub A, Boer R, Urich WR, Lehner MD and Braun C. (2009). Inhibition of inducible nitric oxide synthase in respiratory diseases. Biochemical Society Transactions. 37:886-891. https://doi.org/10.1042/BST0370886
  9. Hsieh CL and Yen GC. (2000). Antioxidant actions of Du-zhong (Eucommia ulmoides OLIV.) toward oxidative damage in biomolecules. Life Sciences. 66:1387-1400. https://doi.org/10.1016/S0024-3205(00)00450-1
  10. Jeoung YJ, Choi SY, An CS, Jeon YH, Park DK and Lim BO. (2009). Comparative effect on anti-inflammatory activity on the Phellinus linteus and Phellinus linteus grown in geminated brown rice extracts in murine macrophage RAW 264.7 cells. Korean Journal of Medicinal Crop Science. 17:97-101.
  11. Kim BH, Park KS and Chang Il-Moo. (2009). Elucidation of anti-inflammatory potencies of Eucommia ulmoides bark and Plantago asiatica seeds. Journal of Medicinal Food. 12:764-769. https://doi.org/10.1089/jmf.2008.1239
  12. Kim HM, Moon EJ, Li E, Kim KM, Nam SY and Chun CK. (1999). The nitric oxide-producing activities of nitric oxide in mouse peritoneal macrophages after priming with interferongamma by the stem of Sinomenium acutum. Journal of Ethnopharmacology. 66:311-317. https://doi.org/10.1016/S0378-8741(98)00218-9
  13. Kim SJ, Jeong HJ, Moon PD, Lee KM, Lee HB, Jung HJ, Rhee HK, Yang DC, Hong SH and Kim HM. (2005). Anti-inflammatory activity of gumiganghwaltang through the inhibition of nuclear factor-kappa B activation in peritoneal macrophages. Biological & Pharmaceutical Bulletin. 28:233-237. https://doi.org/10.1248/bpb.28.233
  14. Kumar P, Paul B, Kumar S, Ali M and Sexana A. (2004). Correlation of cytokine and mobility in mice with arthritis and during therpy with Swertia chirayita. Journal of Herbal Pharmacotherapy. 4:33-45. https://doi.org/10.1080/J157v04n02_04
  15. Kwon SH, Lee HK, Kim JA, Hong SI, Kim SY, Jo TH, Park YI, Lee CK, Kim YB, Lee SY and Jang CG. (2011). Neuroprotective effects of Eucommia ulmoides Oliv. bark on amyloid $beta_{25-35}$-induced learning and memory impairments in mice. Neuroscience Letters. 487:123-127. https://doi.org/10.1016/j.neulet.2010.10.042
  16. Lee YG and Cho JY. (2007). Inhibitory effects of curcumin on nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 cells and its suppressive mechanism. Korean Journal of Medicinal Crop Science. 15:451-456.
  17. Lie H, Chen B and Yao S. (2005). Application of ultrasonic technique for extracting chlorogenic acid from Eucommia ulmodies Oliv. Ultrasonics Sonochemistry. 12:295-300. https://doi.org/10.1016/j.ultsonch.2004.01.033
  18. Lin J, Fan YJ, Mehl C, Zhu JJ, Chen H, Jin LY, Xu JH and Wang HM. (2011). Eucommia ulmoides Oliv. antagonizes $H_2O_2$-induced rat osteoblastic MC3T3-E1 apoptosis by inhibiting expressions of caspases 3, 6, 7, and 9. Journal of Zhejiang University Science B. 12:47-54. https://doi.org/10.1631/jzus.B1000057
  19. Lee SE, Lee JH, Kim JK, Kim GS, Kim YO, Choi JH, Lee ES, Noh HJ, Kim SY and Soe JS. (2011). Anti-inflammatory activity of medicinal plant extracts. Korean Journal of Medicinal Crop Science. 19:217-226. https://doi.org/10.7783/KJMCS.2011.19.4.217
  20. Medzhitov R and Janeway CA Jr. (1997). Innate immunity: the virtues of a nonclonal system of recognition. Cell. 91:295-298. https://doi.org/10.1016/S0092-8674(00)80412-2
  21. Morrison DC and Ryan JL. (1987). Endotoxins and disease mechanisms. Annual Review of Medicine. 38:417-432. https://doi.org/10.1146/annurev.me.38.020187.002221
  22. Nishimoto N and Kishimoto T. (2006). Interleukin 6: from bench to bedside. Nature clinical practice. Rheumatology. 2:619-626.
  23. Park HJ, Jung wt, Basnet P, Kadota S and Namba T. (2004). Syringin 4-O-b-glucoside, a new phenyprosynthase inhibitor, from the stem bark of Magnolia sieboldii. Journal of Natural Products. 59:1128-1130.
  24. Sacco RE, Waters WR, Rudolph KM and Drew ML. (2006). Comparative nitric oxide production by LPS-stimulated monocyte-derived macrophages from Ovis canadensis and Ovis aries. Comparative Immunology, Microbiology and Infectious Diseases. 29:1-11. https://doi.org/10.1016/j.cimid.2005.11.001
  25. Shin M, Chen YD, Shen CR and Cherng. (2010). A molecular pharmacology study into the anti-inflammatory actions of Eluphorbia hirta L. on the LPS-induced RAW 264.7 cells through selective iNOS protein inhibition. Journal of Natural Medicine. 64:330-335. https://doi.org/10.1007/s11418-010-0417-6
  26. Zhu HW, Di HY, Zhang YY, Zhang JW and Chen DF. (2009). A protein-bound polysaccharide from the stem bark of Eucommia ulmoides and its anti-complementary effect. Carbohydrate Research. 344:1319-1324. https://doi.org/10.1016/j.carres.2009.05.001

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