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http://dx.doi.org/10.7783/KJMCS.2012.20.5.381

Anti-inflammatory Effects of Water Extract of Eucommia ulmoides OLIVER on the LPS-induced RAW 264.7 Cells  

Kim, Young-Ock (Department of Herbal Crop Research, NIHHS, RDA)
Lee, Sang-Won (Department of Herbal Crop Research, NIHHS, RDA)
Sohn, Sang-Hyun (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Seung-Yu (Department of Herbal Crop Research, NIHHS, RDA)
Oh, Myung-Sook (Department of Oriental Pharmaceutical Science, College of Pharmacy Kyung Hee University)
Kim, Su-Kang (Kohwang Medical Research Institute, College of Medicine, Kyung Hee University)
Publication Information
Korean Journal of Medicinal Crop Science / v.20, no.5, 2012 , pp. 381-386 More about this Journal
Abstract
Eucommia ulmoides OLIVER (EU) is a traditional Korean herbal used for the treatment of rheumatoid arthritis (RA). In the present study, the molecular pharmacology basis of its anti-inflammatory effect is revealed in this work, EU was studied in lipopolysaccharide (LPS)-activated macrophage cells (RAW 264.7) as an established inflammation model. After activation, nitric oxide (NO) production and iNOS mRNA were measured by using a colorimetric assay (Griess reagent), and reverse transcription polymerase chain reaction (RT-PCR), respectively. The change in the content of $PGE_2$, $TNF{\alpha}$, and IL-6 was concurrently monitored by ELISA. In results, we found that in the concentration range without showing cytotoxicity, EU produced a remarkable anti-inflammatory effect and showed a dose-dependent inhibition of LPSinduced NO production. Compared with indomethacin, EU has more potency and a specific action of NO inhibition, $PGE_2$, IL-6, and TNF-${\alpha}$ inhibition. These results suggest that EU may be a suitable herbal medicine to yield the greatest anti-inflammatory activity for food additives and medicine.
Keywords
Eucommia ulmoides OLIVER; NO; iNOS; $PGE_2$; IL-6; TNF-${\alpha}$;
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