DOI QR코드

DOI QR Code

Heavy Metal Tolerance of Fungi Isolated from Contaminated Soil

  • Joo, Jin-Ho (Department of Biological Environment, Kangwon National University) ;
  • Hussein, Khalid A. (Department of Biological Environment, Kangwon National University)
  • Received : 2012.07.10
  • Accepted : 2012.08.04
  • Published : 2012.08.31

Abstract

This study was conducted to investigate the tolerance of some resistant fungal strains from soils contaminated with heavy metals. Various fungal strains were isolated from soil samples collected from studied sites which heavy metals and other pollutants have been emitted in effluents for several years. Fungi isolated belong to different genera; however, Penicillium spp. showed the most frequent species. The microbial number was remarkably higher in the control soil than contaminated soil samples collected from mining areas. $Pb^{2+}$ and $Zn^{2+}$ had the highest concentration in the polluted soils ranging from 89 - 3,521 ppm and 98 - 4,383 ppm, respectively. The minimum inhibition concentrations (MICs) of $Pb^{+2}$ and $Zn^{+2}$ showed the highest values against the fungal strains. $Ni^{+2}$ and $Co^{+2}$ were the lowest contaminants in the polluted soils with the concentration of 5 to 12.1 ppm and 1.8 to 4.8 ppm, respectively. The tested resistant strains showed the strongest inhibition for $Ni^{+2}$ and $Co^{+2}$ up to 200-400 ppm. Cadmium was the most highly toxic heavy metal for most of strains, however, 1 mM of $Cr^{3+}$, $Cu^{2+}$ and $Pb^{2+}$ accelerated the growth of Penicillium verrucosum KNU3. $Cu^{+2}$ and $Zn^{+2}$ at concentration of 1 mM did not affect the growth rate P. funiculosum KNU4. Tolerance of fungal species to heavy metals appears to be strain and origin dependent.

Keywords

References

  1. Abou-Shanab, R.A. I., P. van Berkum, and J.S. Angle. 2007. Heavy metal resistance and genotypic analysis of metal resistance genes in gram-positive and gram-negative bacteria present in Ni-rich serpentine soil and in the rhizosphere of Alyssum murale. Chemosphere, 68:360-367. https://doi.org/10.1016/j.chemosphere.2006.12.051
  2. Alloway, B.J. 1995. Heavy metal in soils. Second edition, Chapman & Hall, London.
  3. Almas, A.R., L.R. Bakken, and J. Mulder. 2004. Changes in tolerance of soil microbial communities in Zn and Cd contaminated soils. Soil Biol. Biochem., 36:805-813. https://doi.org/10.1016/j.soilbio.2004.01.010
  4. Baldrian, P. 2003. Interactions of heavy metals with white-rot fungi. Enzym. Microb. Technol. 32:78-91. https://doi.org/10.1016/S0141-0229(02)00245-4
  5. Balsalobre, L., M.I. De Siloniz, M.J. Valderrama, T. Benito, M.T. Larrea, and J. M. Peinado. 2003. Occurrence of yeasts in municipal wastes and their behavior in presence of cadmium copper and zinc. J. Basic Microbiol. 43:185-193. https://doi.org/10.1002/jobm.200390021
  6. Canovas, D., C. Duran, N. Rodriguez, R. Amils, and V. Lorenzo. 2003. Testing the limits of biological tolerance to arsenic in a fungus isolated from the River Tinto. Environ. Microbiol. 5(2):133-138. https://doi.org/10.1046/j.1462-2920.2003.00386.x
  7. Chander, K, P.C. Brookes, and S.A. Harding. 1995. Microbial biomass dynamics following addition of metal-enriched sewage sludges to a sandy loam. Soil Biol. Biochem. 27:1049- 1421.
  8. Davis, R.R., W.J. Murphy, J.E. Snawder, C.A. Striley, Henderson, A. Khan, and E.F. Krieg. 2002. 'Susceptibility to the ototoxic properties of toluene is D., species specific', Hear. Res. 166 (1-2) 24-32. https://doi.org/10.1016/S0378-5955(02)00280-0
  9. Diels, L., N. Van der Lelie, and L. Bastiaens. 2002. New development in treatment of heavy metal contaminated soils. Rev. Environ. Sci. Biotechnol. 1:75-82. https://doi.org/10.1023/A:1015188708612
  10. Doelman, P., E. Jansen, M. Michels, and M. Van Til. 1994. Effects of heavy metals in soil on microbial diversity and activity as shown by the sensitivity-resistance index, an ecologically relevant parameters. Biological Fertilized Soil 17:177-184. https://doi.org/10.1007/BF00336319
  11. Domsch, K.H., W. Gams, and T. Anderson. 1980. Compendium of soil fungi Vols 1 and 2. Academic Press. London, pp. 405-859.
  12. Duran, C., I. Marin, and R. Amils. 1999. Specific metal sequestering acidophilic fungi. In: Amils, R.; Ballester, A. (eds). Biohydrometallurgy and the Environment toward the Mining of the 21st Century. Elsevier, Amsterdam. 521-530.
  13. Ezzouhri, L., E. Castro, M. Moya, F. Espinola, and K. Lairini. 2009 Heavy metal tolerance of filamentous fungi isolated from polluted sites in Tangier, Morocco. Afr. J. Microbiol. Res. 3:035-048.
  14. Frostegard, Å., E. Baath, and A.Tunlid. 1993. Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis. Soil Biol. Biochem. 25:723-730. https://doi.org/10.1016/0038-0717(93)90113-P
  15. Gadd, G. M. 1993. Interaction of fungi with toxic metals. New Phytol. 124: 25-60. https://doi.org/10.1111/j.1469-8137.1993.tb03796.x
  16. Gavrilesca, M. 2004. Removal of heavy metals from the environment by biosorption. Eng. Life Sci. 4(3):219-232. https://doi.org/10.1002/elsc.200420026
  17. Gupta, R., P. Ahuja, S. Khan, R.K. Saxena, and H. Mahapatra. 2000. Microbial Biosorbents: Meeting challenges of heavy metal pollution in aqueous solutions. Curr. Sci. 78:967- 973.
  18. Hinojosa, M.B., J.A. Carreira, G.R. Roberto, and P.D. Richard. 2005. Microbial response to heavy metal-polluted soils: Community analysis from phospholipid-linked fatty acids and ester-linked fatty acids extracts. J. Environ. Qual. 34:1789-1800. https://doi.org/10.2134/jeq2004.0470
  19. Holtan-Hartwig, L., P. Dorsch, and L.R. Bakken. 2002. Low temperature control of soil denitrifying communities. Soil Biol. Biochem. 34:1797-1806. https://doi.org/10.1016/S0038-0717(02)00169-4
  20. Itoh, S.M. Iwaki, N. Wakao, K. Yoshizu, A. Aoki, and K. Tazaki. 1998. Accumulation of Fe, Cr and Ni metal inside cells of acidophilic bacterium Acidiphilium rubrum that produces Zn-containing. bacteriochlorophyll a. Plant Cell Physiol. 39(7):740-744, 1998. https://doi.org/10.1093/oxfordjournals.pcp.a029428
  21. Lee, I.S., O.K. Kim, Y.Y. Chang, B. Bae, H.H. Kim, and K.H. Baek. 2002. Heavy metal concentrations and enzyme activities in soil from a contaminated Korean shooting range. J. Biosci. Bioeng. 94(5):406-411. https://doi.org/10.1016/S1389-1723(02)80217-1
  22. Levinskaite, L. 2002. Response of soil fungi to chromium(VI). Ekologija. 1:10-13.
  23. Leyval, C. and E.J Joner. 2001. Bioavailability of heavy metals in the mycorrhizosphere, In G.R. Gobran, W.W. Wenzel, E. Lombi (Eds.), Trace elements in the rhizosphere. CRC, Boca Raton, FL. pp. 165-185.
  24. Lilly, W.W., G.J. Wallweber, and T.A. Lukefahr. 1992. Cadmium absorption and its effects on growth and mycelial morphology of the basidiomycete fungus, Schizophyllum commune. Microbios. 72:227-237.
  25. Mahapatra, N.R. and P.C. Banerjee. 1996. Extreme tolerance to cadmium and high resistance to copper, nickel and zinc in different Acidophilium strains. Lett. Appl. Microbiol. 23: 393-397. https://doi.org/10.1111/j.1472-765X.1996.tb01344.x
  26. Malik, A. 2004. Metal bioremediation through growing cells. Environ. Int. 30: 261-278. https://doi.org/10.1016/j.envint.2003.08.001
  27. Moubasher, A.H. 1993. Soil fungi in Qatar and other Arab countries. The Centre of Scientific and Applied Research, University of Qater, Doha, Qater.
  28. Nakahara, M., Y. Suzuki, and R. Nakamura. 1978. Accumulation of cesium-137 by useful Mollusca. Bull. Jpn. Soc. scient. Fish. 44: 325-329. https://doi.org/10.2331/suisan.44.325
  29. Siloniz, M.E.M. Payo, M.A. Callejo, D. Marquina, and J.M. Peinado. 2002. Environmental adaptation factors of two yeasts isolated from the leachate of a uranium mineral heap. FEMS Microbiol. Lett. 210:233- 237. https://doi.org/10.1016/S0378-1097(02)00607-9
  30. Sumner, M.E. and W.P. Miller. 1996. Cation exchange capacity and exchange coefficients. p. 1201-1229. In D.L. Sparks (ed.) Methods of soil analysis. Part 3. SSSA Book Ser. 5. SSSA, Madison, WI.
  31. Ray, S. and M.K. Ray. 2009. Bioremediation of heavy metal toxicity-with special reference to chromium. J. Med. Sci. 2: 57-63.
  32. USEPA. 1994. SW-846 Method 3051, Microwave Assisted Acid Digestion of Sediments, Sludges, Soils, and Oils. Available online at http://www.epa.gov/epaoswer/hazwaste/test/3xxx.htm.

Cited by

  1. Cellular Response to Cu- and Zn-Induced Oxidative Stress inAspergillus fumigatusIsolated From Polluted Soils in Bulgaria vol.44, pp.6, 2016, https://doi.org/10.1002/clen.201500139
  2. Recent Advances in Bioremediation of Heavy Metals and Metal Complex Dyes: Review vol.142, pp.9, 2016, https://doi.org/10.1061/(ASCE)EE.1943-7870.0000965
  3. Biosorption an innovative tool for bioremediation of metal-contaminated municipal solid waste leachate: optimization and mechanisms exploration vol.14, pp.4, 2017, https://doi.org/10.1007/s13762-016-1173-2
  4. Potential bioremediation of mercury-contaminated substrate using filamentous fungi isolated from forest soil vol.26, pp.6, 2014, https://doi.org/10.1016/S1001-0742(13)60592-6
  5. Potential Use of Aspergillus Flavus Strain KRP1 in Utilization of Mercury Contaminant vol.20, 2014, https://doi.org/10.1016/j.proenv.2014.03.032
  6. vol.42, pp.7, 2018, https://doi.org/10.1039/C8NJ00120K