Korean Journal of Weed Science (한국잡초학회지)

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
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Fragrance, Chemical Composition and Toxicity of the Essential Oil in Erect Bur-marigold (Bidens tripartita L.)

가막사리 (Bidens tripartita L.) 정유의 향취, 화학성분 및 세포독성

  • Yun, Mi-Sun (Department of Biological Environment, Kangwon National University) ;
  • Yeon, Bo-Ram (Department of Biological Environment, Kangwon National University) ;
  • Cho, Hae-Me (Department of Biological Environment, Kangwon National University) ;
  • Lee, Sa-Eun (R&D Center, Hanbul Cosmetics Co. Ltd.) ;
  • Jhoo, Jin-Woo (Department of Animal Products and Food Science, Kangwon National University) ;
  • Jung, Ji-Wook (Department of Herbal Medicinal Pharmacology, Daegu Haany University) ;
  • Park, Yu-Hwa (Gangwon Perfume Alchemy Co. Ltd., Kangwon National University) ;
  • Kim, Song-Mun (Department of Biological Environment, Kangwon National University)
  • 윤미선 (강원대학교 바이오자원환경학과) ;
  • 연보람 (강원대학교 바이오자원환경학과) ;
  • 조해미 (강원대학교 바이오자원환경학과) ;
  • 이사은 (한불화장품주식회사) ;
  • 주진우 (강원대학교 동물식품응용과학과) ;
  • 정지욱 (대구한의대학교 한약재약리학과) ;
  • 박유화 (강원퍼퓸알케미) ;
  • 김성문 (강원대학교 바이오자원환경학과)
  • Received : 2012.08.13
  • Accepted : 2012.09.11
  • Published : 2012.09.30
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Abstract

The essential oil was extracted by steam distillation from the aerial part of erect bur-marigold (Bidens tripartita L.), one of the noxious weed in paddy field. The composition of the essential oil was analyzed by gas chromatography-mass spectrometry. The fragrance of the essential oil was green, herbal, oily, spicy. There were 42 constituents in the essential oil：17 hydrocarbons, 6 alcohols, 6 acetates, 5 N-containing compounds, 3 ethers, 3 ketones, 1 lactone and 1 S-containing compound. Major constituents were ${\alpha}$-phellandrene (22.50%), ${\alpha}$-pinene (22.21%), 2,4-dimethyl (2,5-dimethylphenyl) methyl ester benzoic acid (15.11%), limonene (10.66%), ${\beta}$-pinene (35.43%), and ${\beta}$-cubebene (5.27%). The $IC_{50}$ value in MTT assay using HaCaT keratinocyte cell line was 0.018%. However, attachment of patch with 0.1% of the erect bur-marigold essential oil for 24 hr did not show any skin toxicity. Overall results of this study suggest that the essential oil of erect bur-marigold could be used as a source for the development of perfumery industrial products.

가막사리는 우리나라 전역의 논에서 다발생하여 농업에 막대한 피해를 주는 일년생잡초이지만 이를 활용하는 방안에 대해서는 연구된 바가 없다. 본 연구의 목적은 가막사리 정유를 향장산업제품의 소재로 활용할 수 있는지를 구명하는데 있다. 가막사리에 함유된 정유의 함량은 $0.028{\pm}0.003%$이었으며, 역치값은 0.1%이었으며, 향취는 green, herbal, oily, spicy이었다. 가막사리 정유에는 탄화수소 17종, 알코올 6종, 아세테이트 6종, 질소화합물 5종, 에테르 3종, 케톤 3종, 락톤 1종, 황화합물 1종 등 총 42종의 화학성분이 함유되어 있었으며, 다함량 성분은 ${\alpha}$-phellandrene(22.50%), ${\alpha}$-pinene(22.21%), 2,4-dimethyl(2,5-dimethylphenyl)methyl ester benzoic acid(15.11%), limonene(10.66%), ${\beta}$-pinene(35.43%), ${\beta}$-cubebene(5.27%)이었다. 가막사리 정유가 HaCaT 각질형성세포에 미치는 세포독성을 조사하기 위한 MTT assay를 실시한 결과, 가막사리 정유의 $IC_{50}$값은 0.018%이었으며, 피부에 미치는 영향을 조사하기 위하여 피부자극유발시험을 수행한 결과 가막사리 정유 0.1% 처리에도 그 어떠한 발진이나 수포도 발견되지 않았다. 본 연구의 결과는 가막사리 정유가 향장제품을 위한 소재로 활용될 수 있다는 점을 시사하여 준다.

Keywords

References

  1. Cho, C. H., J. C. Lee, Y. H. Kim, K. S. Kim, T. J. Ahn, and O. K. Han. 1996. Odor characteristics of essential oil of Valeriana fauriei var. dasycarpa HARA. Korean J. Medicinal Crop Sci. 4(1):31-37.
  2. Cho, M. G., J. K. Bang, and Y. A. Chae. 2003. Comparison of volatile compounds in plant parts of Angelica gigas Nakai and A. acutiloba Kitagawa. Korean J. Medicinal Crop Sci. 11(5):352-357.
  3. Choi, C. D., B. C. Moon, S. C. Kim, and Y. J. Oh. 1995. Weed growth and effective control in directseeded rice fields. Korean J. Weed Sci. 15(3): 175-182.
  4. Choi, H. J., H. Y. Wang, Y. N. Kim, S. J. Heo, N. K. Kim, M. S. Jeong, Y. H. Park, and S. Kim. 2008. Composition and cytotoxicity of essential oil extracted by steam distillation from horseweed (Erigeron canadensis L.) in Korea. J. Korean Soc. Appl. Biol. Chem. 51(1):55-59.
  5. Fujimaki, M. 1982. Kouryounozitencin (in Japanese). Asakurashoten, Tokyo, Japan, p. 368.
  6. Heinrich, G., P. I. Plawer, J. R. Wegener, and W. Schultz. 1989. Compartmentation of the biosynthesis of the essential oil in poncirus and monarda. In Aetherische Oele, Ergeb. Int, Arbeitstage. Stuttgart, Fed. Rep. Ger. p.198.
  7. Hong, C. U. 2004. Comparison of essential oil composition of Artemisa iwayomogi and Artemisia capillaris. J. Korean Soc. Appl. Biol. Chem. 47(1):124-129.
  8. Hwang, I. T., J. S. Choi, H. H. Kuack, J. S. Kim, H. J. Lee, and K. Y. Cho. 1996. Seed germination, seedling growth, and herbicidal responses of Bidens tripartita L. Korean J. Weed Sci. 16(2):114-121.
  9. Kim, S. 2008. Composition and cell cytotoxicity of essential oil from Caryopteris incana Miq. in Korea. J. Kor. Soc. Appl. Biol. Chem. 51(3):238- 244.
  10. Kim, S. C. 1992. Weed ecology and effective weed control technology in direct-seeded rice. Korean J. Weed Sci. 12(3):230-260.
  11. Kim, S., H. Y. Kim, K. H. Hwang, and I. J. Chun. 2008a. Herbicidal activity of essential oil from Glechoma hederacea. Korean J. Weed Sci. 28(2): 152-160.
  12. Kim, S., J. M. Yu, H. T. Lim, and H. Y. Wang. 2008b. Chemical composition of floral essential oils of five potato (Solanum tuberosum L.) cultivars. J. Appl. Biol. Chem. 51(5):177-178. https://doi.org/10.3839/jabc.2008.037
  13. Lee, C. B. 2003. Coloured Flora of Korea. Vol. II. pp. 355-357.
  14. Lee, M. S. and M. S. Chung. 2001. Analysis of volatile flavor components from Allium senescens. Korean J. Soc. Food Sci. 17(1):55-59.
  15. Lee, Y. M. 2002. Studies on aroma-active compounds in Japanese apricot. Master of Science Thesis. Changwon National University. 83p.
  16. Leem, Y. H. 2001. Aroma-active compounds of 15 aroma plants in Korea and aroma extract dilution analysis of Artemisia montana Nakai, Isodon excisus Maxim and Schizandra chinensis Baill. Master of Science Thesis. Seoul National University. 44p.
  17. Mosciano, G. 2000. Organoleptic characteristics of flavor materials. Perfume Flavor 25:25-31.
  18. Park, T. S., J. E. Park, G. H. Ryu, I. Y. Lee, H. K. Lee, and J. O. Lee. 1995. Effective weed control in directed-seeded rice under dry fields. Korean J. Weed Sci. 15(2):99-104.
  19. Park, Y. H. and S. Kim. 2008. Composition and cytotoxicity of essential oil from Korean rhododendron (Rhododendon mucronulatum Turcz. var. ciliatum Nakai). J. Korean Soc. Appl. Biol. Chem. 51(3):233-237.
  20. Schmidt, E. 2010. Production of essential oils. pp. 83-119. In : Handbook of Essential Oils. CRC Press.
  21. Song, H. S., Y. H. Park, and D. G. Moon 2005. Volatile flavor properties of Hallabong grown in open field and green house by GC/GC-MS and sensory evaluation. J. Korean Soc Food Sci Nutr 34(8):1239-1245. https://doi.org/10.3746/jkfn.2005.34.8.1239
  22. Yeon, B. R., S. J. Park, M. S. Jeong, and S. Kim. 2011a. Fragrance and chemical composition of essential oil in flowers of Magnolia (Magnolia obovata Thunb.). J. Agric. Life Environ. Sci. 23(3):74-76.
  23. Yeon, B. R., H. M. Cho, M. S. Jeong, and S. Kim. 2011b. Fragrance and chemical composition of essential oil in corn of metasequoia (Metasequoia glyptostroboides Hu et Cheng). Korean J. Weed Sci. 31(2):146-151. https://doi.org/10.5660/KJWS.2011.31.2.146
  24. Yeon, B. R., S. E. Lee, H. S. Noh, and S. Kim. 2011c. Fragrance and chemical composition of essential oil of Aster yomena Makino in Gangwon, Korea. J. Agric. Life Environ. Sci. 23(1):16-21.
  25. Yu, J. M. 2010. Volatile fragrance components in Aralia cordata Thunb.：Their seasonal changes, toxicity and effects on electroencephalogram. Master of Science Thesis. Kangwon National University. 102p.
  26. Yu, J. M., Y. H. Park, and S. Kim. 2008. Floral volatile composition of diasy fleabane (Erigeron annuus (L.) Pers.). Korean J. Weed Sci. 28(3): 274-278.