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http://dx.doi.org/10.6116/kjh.2012.27.5.15

The Antioxidant Activity of Nelumbinis Stamen in GC-2 spd(ts) Cells  

Park, Eun-Hwa (Dept. of Herbal Pharmaceutical Science, Joongbu University)
Chang, Mun-Seog (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Kil, Ki-Jeong (Dept. of Herbal Pharmaceutical Science, Joongbu University)
Park, Seong-Kyu (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Publication Information
The Korea Journal of Herbology / v.27, no.5, 2012 , pp. 15-20 More about this Journal
Abstract
Objectives : The purpose of this study was to estimate the antioxidant activity of water extract of Nelumbinis stamen (WNS) in GC-2 spd (ts) cells. Methods : we investigated the effect of WNS in mouse GC-2 spd (ts) cells by MTT assay. The protective effects of WNS against hydrogen peroxide-induced oxidative stress in GC-2 spd (ts) cells were examined by measuring cell viability. Lipid peroxidation levels and catalase were measured. Results : WNS showed cell viability as 101.9, 108.9, 111.8, 125.8, 134.5% in 2.5, 5, 10, 25, 50 ${\mu}g/ml$ concentrations, respectively. The protective effect of WNS concentration was 2.5 ${\mu}g/ml$ against hydrogen peroxide-induced oxidative stress in GC-2 spd (ts) cells. LPO were decreased significantly at 2.5, 5, 25 ${\mu}g/ml$ of WNS concentrations. Catalase activity was significantly increased at 2.5, 5 and 10 ${\mu}g/ml$ of WNS concentrations, respectively. Conclusions : In conclusion, WNS has antioxidant activities in GC-2 spd(ts) cells against oxidative stress.
Keywords
water extract of Nelumbinis stamen (WNS); GC-2 spd(ts) cells; Cell viability; LPO; Catalase;
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