아시안잔디학회지 (Asian Journal of Turfgrass Science)

한국잔디학회 (Turfgrass Society of Korea)
권호 표지

들잔디 갈색퍼짐병의 생물학적 방제를 위한 길항 세균의 분리와 동정

Isolation and Identification of Antagonistic Bacteria for Biological Control of Large Patch Disease of Zoysiagrass Caused by Rhizoctonia solani AG2-2 (IV)

  • 송치헌 (대구대학교 생물공학과) ;
  • ;
  • 장태현 (경북대학교 식물자원환경전공) ;
  • 이용세 (대구대학교 생명환경학부)
  • Song, Chi-Hyun (Department of Biotechnology, Daegu University) ;
  • Islam, Md. Rezuanul (Department of Biotechnology, Daegu University) ;
  • Chang, Tae-Hyun (Plant Resources and Environment Major, Kyungpook National University) ;
  • Lee, Yong-Se (Division of Life and Environmental Science, Daegu University)
  • 투고 : 2012.03.14
  • 심사 : 2012.04.05
  • 발행 : 2012.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

한국 들잔디에 발생하는 Rhizoctonia solani에 의한 라지패치를 생물학적으로 방제하기위해 일반토양에서 길항미생물을 분리하여 in vitro와 in vivo에서 길항효과 및 병 발생억제효과를 검정하였다. 토양에서 분리한 216개 균주 중 15개 균주가 R. solani AG2-2 (IV)의 균사생장을 70%이상 억제하였으며, 온실실험 결과 11개 균주는 잔디의 생장을 촉진시켰으며, 병 발생 억제효과가 있었다. 분리한 길항미생물 중 H33 균주는 in vitro 및 in vivo에서 R. solani AG2-2 (IV)에 대한 길항효과가 다른 균주에 비해 높았으며, 공시한 17개 식물병원성 진균에 대해 길항효과가 높아 생물방제균으로 선발하였다. H33 균주를 ISP 배지에 배양한 후 배양적 특성 및 형태를 관찰한 결과 Streptomyces sp.로 동정되었으며, 16S rDNA를 분석한 결과 Streptomyces arenae와 99% 상동성을 보였다.

The objective of this study was to identify bacterial antagonists of R. solani AG2-2 (IV) on zoysiagrass and to evaluate their antifungal activity in vitro and in vivo to select an antagonistic isolate. Antagonistic isolates that inhibit large patch disease caused by R. solani AG2-2 (IV) in zoysiagrass were selected from several soils, and their antagonistic activities were investigated in vitro and in vivo. Of 216 bacterial isolates, 67 inhibited several plant pathogenic fungi. The isolates that inhibited stem-segment colonization by R. solani AG2-2 (IV) in zoysiagrass were tested in a growth chamber. Eleven isolates were active as plant growth promoting isolates. Among them, five plant growth promoting isolates and their concentration dependent efficiency on zoysiagrass following inoculation with R. solani AG2-2 (IV) was evaluated. Isolate H33 was one of the potential antagonistic isolates, and it was further tested against various plant pathogens. H33 not only suppressed the disease caused by R. solani AG2-2 (IV) on zoysiagrass but also promoted leaf weight and leaf height of zoysiagrass under growth chamber and greenhouse conditions. The H33 isolate, which belongs to Streptomyces arenae, was identified through physiological, biochemical, and 16S rDNA studies. Further studies will investigate the cultural characterization of S. arenae H33 and isolation and identification of antifungal substance produced by S. arenae H33.

키워드

참고문헌

  1. Alabouvette, C. 1986. Fusarium wilt-suppressive soils from the Chateaurenard region: review of a 10-year study. Agronomie 6:273-284. https://doi.org/10.1051/agro:19860307
  2. Anith, K.N., N.V. Radhakrishnam, and T.P. Manomohandas. 2003. Screening of antagonistic bacteria for biological control of nursery wilt of black pepper (Piper nigrum). Microbiological Research 15:91-97.
  3. Berg, G., N. Roskot, A. Steidle, L. Eber, A. Zock, and K. Smalla. 2002. Plant-dependent genotypic and phenotypic diversity of antagonistic rhizobacteria isolated from different Verticillium host plants. Applied and Environmental Microbiology 68:3328-3338. https://doi.org/10.1128/AEM.68.7.3328-3338.2002
  4. Berg, G., A. Krechel, M. Ditz, R. Sikora, A. Ulrich, and J. Hallmann. 2005. Endophytic and ectophytic potato-associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi. FEMS Microbiol. Eco. 51:215-229. https://doi.org/10.1016/j.femsec.2004.08.006
  5. Berg, G., K. Opelt, C. Zachow, J. Lottmann, M. Gotz, R. Costa, and K. Smalla. 2006. The rhizosphere effect on bacteria antagonistic towards the pathogenic fungus Verticillium differs depending on plant species and site. FEMS Microbiol. Eco. 56:250-261. https://doi.org/10.1111/j.1574-6941.2005.00025.x
  6. Chang, T.H. and Y.S. Lee. 2010. Evaluation of occurrence of yellow patch caused by Rhizoctonia cerealis of cool season turfgrass cultivars and species. Kor. Turfgrass Sci. 24(1):24-30. (in Korean)
  7. Compant, S., B. Duffy, J. Nowak, C. Clement, and E. Barka. 2005. Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action and future prospects. App. Environ. Microbiol. 71: 4951-4959. https://doi.org/10.1128/AEM.71.9.4951-4959.2005
  8. Cook, R.J. and A.D. Rovira. 1976. The role of bacteria in the biological control of Gaeumannomyces graminis by suppressive soils. Soil Biology & Biochemistry 8:269-274. https://doi.org/10.1016/0038-0717(76)90056-0
  9. Dahiya, N. 2005. Production of an antifungal chitinase from Enterobacter sp. NRG4 and its application in protoplast production. World J. Microbiol. Biotechnol. 21:8-9.
  10. Elad, Y., I. Chet, and Y. Henis, 1982. Degradation of plant pathogenic fungi by Trichoderma harzianum. Canadian journal of Microbiology 28:719-725. https://doi.org/10.1139/m82-110
  11. Fravel, D.R. 1988. Role of antibiosis in the biocontrol of plant diseases. Annual Review of Phytopathology 26:75-91. https://doi.org/10.1146/annurev.py.26.090188.000451
  12. Fravel, D.R. 2005. Commercialization and implementation of biocontrol, Annual Review of Phytopathology 43:337-359. https://doi.org/10.1146/annurev.phyto.43.032904.092924
  13. Garbeva, P., J.A. Veen, and J.D. Elsas. 2004. Assessment of the diversity, and antagonism towards Rhizoctonia solani AG3, of Pseudomonas species in soil from different agricultural regimes. FEMS Microbiol Eco. 47:51-64. https://doi.org/10.1016/S0168-6496(03)00234-4
  14. Gerhardson, B. 2002. Biological substitutes for pesticides. Trends in Biotechnol. 20:338-343. https://doi.org/10.1016/S0167-7799(02)02021-8
  15. Goodfellow, M., S.T. Williams, and M. Mordarski. 1988. Actinomycetes in Biotechnology. pp. 130-175, Academic Press, London.
  16. Han, D.Y., D.L. Coplin, W.D. Bauer, and H.A. Hoitink. 2000. A rapid bioassay for screening rhizosphere microorganisms for their ability to induce systemic resistance, Phytopathology 90:327-332. https://doi.org/10.1094/PHYTO.2000.90.4.327
  17. Heyrman, J. and J. Swings. 2001. 16S rDNA sequence analysis of bacterial isolates from biodeteriorated mural paintings in the servilia tomb (Necropolis of Carmona, Seville, Spain) system. App. Microbiology 24:417-422. https://doi.org/10.1078/0723-2020-00048
  18. Jung, W.Y., K.N. An, Y.L. Jin, R.D. Park, K.T. Lim, and K.Y. Kim. 2003. Bioloical control of damping-off caused by Rhizoctonia solani using chitinase-producing Paenibacillus illinoiseniss KJ-424, Soil biology Biotechemistry 35:1261-1264. https://doi.org/10.1016/S0038-0717(03)00187-1
  19. Lugtenberg, B.J.J., L. Dekkers, and G.V. Bloemberg. 2001. Molecular determinants of rhizosphere colonization by Pseudomonas. Annual Review of Phytopatholgy 39:461-490. https://doi.org/10.1146/annurev.phyto.39.1.461
  20. Locci, R. 1989. Streptomyces and related genera, In: Bergey''s manual of systematic bacteriology, pp. 2451-2508, fourth. ed. Williams & Wilkins Company, Baltimore.
  21. Mellouli, L., R.B. Mehdi, S. Sioud, M. Salem, and S. Bejar. 2003. Isolation, purification and partial characterization of antibacterial activities produced by a newly isolated Streptomyces sp. US24 strain. Res. Microbiol. 154:345-352. https://doi.org/10.1016/S0923-2508(03)00077-9
  22. Nolan, R. and T. Cross. 1998. Isolation and screening of actinomycetes, in: Goodfellow, M., Williams, S. T., Mordarski, M. (Eds). Actinomycetes in biotechnology. Academic Press, London, pp. 33-67.
  23. Okami, Y. and T. Hott. 1988. Search and discovery of new antibiotics. in: Goodfellow, M., Williams, S. T., Mordarski, M. (Eds), Actinomycetes in biotechnology. Academic Press, Inc., New York, pp. 33-67.
  24. Singh, P., Y.C. Shin, C.S. Park, and Y.R. Chung. 1999. Biological control of Fusarium wilt of cucumber by chitinolytic bacteria. Phytopathology 89:92-99. https://doi.org/10.1094/PHYTO.1999.89.1.92
  25. Tamura, M., Tsushida, T., Shinohara, K., 2007. Isolation of an isoflavone-metabolizing, Clostridium-like bacterium, strain TM-40, from human faces. Physiology microbial chemistry, Anaerobe 13:32-35.
  26. Welbaum, G., A.V. Sturz, Z. Dong, and J. Nowak. 2004. Fertilizing soil microorganisms to improve productivity of agroecosystems, Critical Reviews in Plant Sciences 23:175-193. https://doi.org/10.1080/07352680490433295
  27. Worku, Y. and B. Gerhardson, 1996. Suppressiveness to clubroot, pea root and Fusarium wilt in Swedish soils. Journal of Phytopathology 144:143-146. https://doi.org/10.1111/j.1439-0434.1996.tb01504.x