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Isolation and Identification of Antagonistic Bacteria for Biological Control of Large Patch Disease of Zoysiagrass Caused by Rhizoctonia solani AG2-2 (IV)  

Song, Chi-Hyun (Department of Biotechnology, Daegu University)
Islam, Md. Rezuanul (Department of Biotechnology, Daegu University)
Chang, Tae-Hyun (Plant Resources and Environment Major, Kyungpook National University)
Lee, Yong-Se (Division of Life and Environmental Science, Daegu University)
Publication Information
Asian Journal of Turfgrass Science / v.26, no.1, 2012 , pp. 8-16 More about this Journal
Abstract
The objective of this study was to identify bacterial antagonists of R. solani AG2-2 (IV) on zoysiagrass and to evaluate their antifungal activity in vitro and in vivo to select an antagonistic isolate. Antagonistic isolates that inhibit large patch disease caused by R. solani AG2-2 (IV) in zoysiagrass were selected from several soils, and their antagonistic activities were investigated in vitro and in vivo. Of 216 bacterial isolates, 67 inhibited several plant pathogenic fungi. The isolates that inhibited stem-segment colonization by R. solani AG2-2 (IV) in zoysiagrass were tested in a growth chamber. Eleven isolates were active as plant growth promoting isolates. Among them, five plant growth promoting isolates and their concentration dependent efficiency on zoysiagrass following inoculation with R. solani AG2-2 (IV) was evaluated. Isolate H33 was one of the potential antagonistic isolates, and it was further tested against various plant pathogens. H33 not only suppressed the disease caused by R. solani AG2-2 (IV) on zoysiagrass but also promoted leaf weight and leaf height of zoysiagrass under growth chamber and greenhouse conditions. The H33 isolate, which belongs to Streptomyces arenae, was identified through physiological, biochemical, and 16S rDNA studies. Further studies will investigate the cultural characterization of S. arenae H33 and isolation and identification of antifungal substance produced by S. arenae H33.
Keywords
Biological control; Large patch; Zoysiagrass; Rhizoctonia solani; Streptomyces arenae;
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1 Garbeva, P., J.A. Veen, and J.D. Elsas. 2004. Assessment of the diversity, and antagonism towards Rhizoctonia solani AG3, of Pseudomonas species in soil from different agricultural regimes. FEMS Microbiol Eco. 47:51-64.   DOI   ScienceOn
2 Gerhardson, B. 2002. Biological substitutes for pesticides. Trends in Biotechnol. 20:338-343.   DOI   ScienceOn
3 Goodfellow, M., S.T. Williams, and M. Mordarski. 1988. Actinomycetes in Biotechnology. pp. 130-175, Academic Press, London.
4 Han, D.Y., D.L. Coplin, W.D. Bauer, and H.A. Hoitink. 2000. A rapid bioassay for screening rhizosphere microorganisms for their ability to induce systemic resistance, Phytopathology 90:327-332.   DOI   ScienceOn
5 Heyrman, J. and J. Swings. 2001. 16S rDNA sequence analysis of bacterial isolates from biodeteriorated mural paintings in the servilia tomb (Necropolis of Carmona, Seville, Spain) system. App. Microbiology 24:417-422.   DOI   ScienceOn
6 Jung, W.Y., K.N. An, Y.L. Jin, R.D. Park, K.T. Lim, and K.Y. Kim. 2003. Bioloical control of damping-off caused by Rhizoctonia solani using chitinase-producing Paenibacillus illinoiseniss KJ-424, Soil biology Biotechemistry 35:1261-1264.   DOI   ScienceOn
7 Lugtenberg, B.J.J., L. Dekkers, and G.V. Bloemberg. 2001. Molecular determinants of rhizosphere colonization by Pseudomonas. Annual Review of Phytopatholgy 39:461-490.   DOI   ScienceOn
8 Locci, R. 1989. Streptomyces and related genera, In: Bergey''s manual of systematic bacteriology, pp. 2451-2508, fourth. ed. Williams & Wilkins Company, Baltimore.
9 Mellouli, L., R.B. Mehdi, S. Sioud, M. Salem, and S. Bejar. 2003. Isolation, purification and partial characterization of antibacterial activities produced by a newly isolated Streptomyces sp. US24 strain. Res. Microbiol. 154:345-352.   DOI   ScienceOn
10 Nolan, R. and T. Cross. 1998. Isolation and screening of actinomycetes, in: Goodfellow, M., Williams, S. T., Mordarski, M. (Eds). Actinomycetes in biotechnology. Academic Press, London, pp. 33-67.
11 Okami, Y. and T. Hott. 1988. Search and discovery of new antibiotics. in: Goodfellow, M., Williams, S. T., Mordarski, M. (Eds), Actinomycetes in biotechnology. Academic Press, Inc., New York, pp. 33-67.
12 Singh, P., Y.C. Shin, C.S. Park, and Y.R. Chung. 1999. Biological control of Fusarium wilt of cucumber by chitinolytic bacteria. Phytopathology 89:92-99.   DOI   ScienceOn
13 Tamura, M., Tsushida, T., Shinohara, K., 2007. Isolation of an isoflavone-metabolizing, Clostridium-like bacterium, strain TM-40, from human faces. Physiology microbial chemistry, Anaerobe 13:32-35.
14 Welbaum, G., A.V. Sturz, Z. Dong, and J. Nowak. 2004. Fertilizing soil microorganisms to improve productivity of agroecosystems, Critical Reviews in Plant Sciences 23:175-193.   DOI   ScienceOn
15 Berg, G., N. Roskot, A. Steidle, L. Eber, A. Zock, and K. Smalla. 2002. Plant-dependent genotypic and phenotypic diversity of antagonistic rhizobacteria isolated from different Verticillium host plants. Applied and Environmental Microbiology 68:3328-3338.   DOI   ScienceOn
16 Worku, Y. and B. Gerhardson, 1996. Suppressiveness to clubroot, pea root and Fusarium wilt in Swedish soils. Journal of Phytopathology 144:143-146.   DOI   ScienceOn
17 Alabouvette, C. 1986. Fusarium wilt-suppressive soils from the Chateaurenard region: review of a 10-year study. Agronomie 6:273-284.   DOI
18 Anith, K.N., N.V. Radhakrishnam, and T.P. Manomohandas. 2003. Screening of antagonistic bacteria for biological control of nursery wilt of black pepper (Piper nigrum). Microbiological Research 15:91-97.
19 Berg, G., A. Krechel, M. Ditz, R. Sikora, A. Ulrich, and J. Hallmann. 2005. Endophytic and ectophytic potato-associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi. FEMS Microbiol. Eco. 51:215-229.   DOI   ScienceOn
20 Berg, G., K. Opelt, C. Zachow, J. Lottmann, M. Gotz, R. Costa, and K. Smalla. 2006. The rhizosphere effect on bacteria antagonistic towards the pathogenic fungus Verticillium differs depending on plant species and site. FEMS Microbiol. Eco. 56:250-261.   DOI   ScienceOn
21 Chang, T.H. and Y.S. Lee. 2010. Evaluation of occurrence of yellow patch caused by Rhizoctonia cerealis of cool season turfgrass cultivars and species. Kor. Turfgrass Sci. 24(1):24-30. (in Korean)
22 Compant, S., B. Duffy, J. Nowak, C. Clement, and E. Barka. 2005. Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action and future prospects. App. Environ. Microbiol. 71: 4951-4959.   DOI   ScienceOn
23 Fravel, D.R. 1988. Role of antibiosis in the biocontrol of plant diseases. Annual Review of Phytopathology 26:75-91.   DOI   ScienceOn
24 Cook, R.J. and A.D. Rovira. 1976. The role of bacteria in the biological control of Gaeumannomyces graminis by suppressive soils. Soil Biology & Biochemistry 8:269-274.   DOI   ScienceOn
25 Dahiya, N. 2005. Production of an antifungal chitinase from Enterobacter sp. NRG4 and its application in protoplast production. World J. Microbiol. Biotechnol. 21:8-9.
26 Elad, Y., I. Chet, and Y. Henis, 1982. Degradation of plant pathogenic fungi by Trichoderma harzianum. Canadian journal of Microbiology 28:719-725.   DOI
27 Fravel, D.R. 2005. Commercialization and implementation of biocontrol, Annual Review of Phytopathology 43:337-359.   DOI   ScienceOn