Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Computational Tridimensional Protein Modeling of Cry1Ab19 Toxin from Bacillus thuringiensis BtX-2

  • Kashyap, S. (National Bureau of Agriculturally Important Microorganisms (ICAR)) ;
  • Singh, B.D. (School of Biotechnology, Faculty of Science, Banaras Hindu University) ;
  • Amla, D.V. (Molecular Biology and Genetic Engineering Division, National Botanical Research Institute)
  • Received : 2011.06.15
  • Accepted : 2012.02.02
  • Published : 2012.06.28
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Abstract

We report the computational structural simulation of the Cry1Ab19 toxin molecule from B. thuringiensis BtX-2 based on the structure of Cry1Aa1 deduced by x-ray diffraction. Validation results showed that 93.5% of modeled residues are folded in a favorable orientation with a total energy Z-score of -8.32, and the constructed model has an RMSD of only $1.13{\AA}$. The major differences in the presented model are longer loop lengths and shortened sheet components. The overall result supports the hierarchical three-domain structural hypothesis of Cry toxins and will help in better understanding the structural variation within the Cry toxin family along with facilitating the design of domain-swapping experiments aimed at improving the toxicity of native toxins.

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References

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