Journal of Sasang Constitutional Medicine (사상체질의학회지)

The Society Of Sasang Constitutional Medicine (사상체질의학회)
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Effects of TAM (Taraxacum mongolicum) on Th2 Cytokine Production in MC/9 Mast Cells

포공영(蒲公英)이 MC/9 mast cell에서의 Th2 cytokine 발현에 미치는 영향

  • Jang, Moon-Hee (Hot Spring Medicine Laboratory, College Oriental Medicine, Daejeon Univ.) ;
  • Choi, Jae-Song (Dept. of Sasang Constitutional Medicine College Oriental Medicine, Daejeon Univ.) ;
  • Bae, Na-Young (Hot Spring Medicine Laboratory, College Oriental Medicine, Daejeon Univ.) ;
  • Ahn, Teak-Won (Hot Spring Medicine Laboratory, College Oriental Medicine, Daejeon Univ.)
  • 장문희 (대전대학교 온천의학연구소) ;
  • 최재송 (대전대학교 한의과대학 사상체질의학과) ;
  • 배나영 (대전대학교 온천의학연구소) ;
  • 안택원 (대전대학교 온천의학연구소)
  • Received : 2012.02.09
  • Accepted : 2012.03.07
  • Published : 2012.03.31
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Abstract

1. Objective : The purpose of this study is to investigate the effects of TAM (Taraxacum mongolicum) on Th2 cytokine production in MC/9 mast cells. 2. Methods : The effects of TAM was analyzed by ELISA and Real-time PCR in MC/9 mast cells. Levels of IL-5, IL-13 were measured using enzyme-linked immunosorbent assays(ELISA). mRNA levels of IL-4, IL-5, IL-6, IL-13 were analyzed with Real-time PCR. 3. Results : 1) TAM inhibited the IL-4 production significantly in comparison to PI-control group at concentration of $50{\mu}g/ml$, $100{\mu}g/ml$, $200{\mu}g/ml$. 2) TAM inhibited the IL-13 production significantly in comparison to PI-control group at concentration of $50{\mu}g/ml$, $100{\mu}g/ml$, $200{\mu}g/ml$. 3) TAM inhibited the IL-4 mRNA expression significantly in comparison to PI-control group at concentration of $100{\mu}g/ml$. 4) TAM inhibited the IL-5 mRNA expression significantly in comparison to PI-control group at concentration of $50{\mu}g/ml$, $100{\mu}g/ml$. 5) TAM inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of $100{\mu}g/ml$. 6) TAM inhibited the IL-13 mRNA expression significantly in comparison to PI-control group at concentration of $100{\mu}g/ml$. 4. Conclusions : These results indicate that TAM (Taraxacum mongolicum) has the effect of decreasing the Th2 cytokine production in the MC/9 mast cell.

Keywords

References

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