Browse > Article
http://dx.doi.org/10.7730/JSCM.2012.24.1.054

Effects of TAM (Taraxacum mongolicum) on Th2 Cytokine Production in MC/9 Mast Cells  

Jang, Moon-Hee (Hot Spring Medicine Laboratory, College Oriental Medicine, Daejeon Univ.)
Choi, Jae-Song (Dept. of Sasang Constitutional Medicine College Oriental Medicine, Daejeon Univ.)
Bae, Na-Young (Hot Spring Medicine Laboratory, College Oriental Medicine, Daejeon Univ.)
Ahn, Teak-Won (Hot Spring Medicine Laboratory, College Oriental Medicine, Daejeon Univ.)
Publication Information
Journal of Sasang Constitutional Medicine / v.24, no.1, 2012 , pp. 54-65 More about this Journal
Abstract
1. Objective : The purpose of this study is to investigate the effects of TAM (Taraxacum mongolicum) on Th2 cytokine production in MC/9 mast cells. 2. Methods : The effects of TAM was analyzed by ELISA and Real-time PCR in MC/9 mast cells. Levels of IL-5, IL-13 were measured using enzyme-linked immunosorbent assays(ELISA). mRNA levels of IL-4, IL-5, IL-6, IL-13 were analyzed with Real-time PCR. 3. Results : 1) TAM inhibited the IL-4 production significantly in comparison to PI-control group at concentration of $50{\mu}g/ml$, $100{\mu}g/ml$, $200{\mu}g/ml$. 2) TAM inhibited the IL-13 production significantly in comparison to PI-control group at concentration of $50{\mu}g/ml$, $100{\mu}g/ml$, $200{\mu}g/ml$. 3) TAM inhibited the IL-4 mRNA expression significantly in comparison to PI-control group at concentration of $100{\mu}g/ml$. 4) TAM inhibited the IL-5 mRNA expression significantly in comparison to PI-control group at concentration of $50{\mu}g/ml$, $100{\mu}g/ml$. 5) TAM inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of $100{\mu}g/ml$. 6) TAM inhibited the IL-13 mRNA expression significantly in comparison to PI-control group at concentration of $100{\mu}g/ml$. 4. Conclusions : These results indicate that TAM (Taraxacum mongolicum) has the effect of decreasing the Th2 cytokine production in the MC/9 mast cell.
Keywords
TAM (Taraxacum mongolicum); Cytokine; MC/9 Mast Cell; Atopic Dermatitis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Toshiaki Kawakami, Stephen J. Galli. Regulation of mast-cell and basophil function and survival by IgE. Nature Reviews Immunology. 2002;2:773-786.   DOI   ScienceOn
2 Chang TW, Shiung YY. Anti IgE as a mast cell-stabilizing therapeutic agent. The Journal of Allergy and Clinical Immunology. 2006;117(6):1203-1212.   DOI   ScienceOn
3 Katx HR, Stevens RL, Austen KF. Heterogeneity of mammalian mast cells differentiates in vivo and in vitro. J Allergy Clin Immunol. 1985;76:250-259.   DOI
4 Abul K. Abbas, Andrew H. Lichtman. 최신면역학입문 3판. Seoul:E public. 2009:205-212.
5 Kinet JP. The high-affinity IgE receptor (FcεRI) : from physiology to pathology. Annu Rev Immunol. 1999;17:931-972.   DOI   ScienceOn
6 Marquardt DL, Wasserman SI. Modulation of rat serosal mast cell biochemistry by in vivo dexamethasone administration. J Immunol. 1983;131:934-939.
7 Taketomi Y, Murakami M. Lipid networks in mast cell biology. Yakugaku Zasshi. 2001;131(1):73-84.
8 Park SB, Kang KH, Yoon HJ, Ko WS. Inhibitiry effect of Ulmus davidiana on β-hexosaminidase release and cytokine production in RBL-2H3 cells. The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 2011;24(1):86-95. (Korean)
9 Son MJ. Suppressive effect of SangRyoSaMulTang on activated of RBL-2H3 mast cells. Graduate school of Daejeon University master's thesis. 2011:1-29.(Korean)
10 Bennett, W.M. and Noeman, D.J. Action and toxicity of cyclosporin. Annu. Rev. Med. 1986;37:215-224. (Korean)
11 Lee JS, Yun SJ, Lee JB, Kim SJ, Won YH, Lee SC. The Efficacy of Cyclosporin in Patients with Atopic Dermatitis. Korean Journal of dermatology. 2008; 46(2):224-230.(Korean)
12 Noh KH. Inhibitory effect of Taraxaci Herba Extract(THE) on pro-inflammatory mediatory. Graduate school of Daegu Haany University master's thesis. 2009:1-32.(Korean)
13 Jo JH. The study of Taraxici Herba on the Anti-allergic Effects. Graduate school of Dongeui University doctor's thesis. 2007:1-33.(Korean)
14 Munetaka Ishiyama, Yoko Miyazono, Kazumi Sasamoto, Yosuke Ohkura and Keiyu Ueno. A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability. Talanta. 1997;44(7):1299-1305.   DOI   ScienceOn
15 Akdis A, Akdis M, Blaser K. Immune regulation in atopic dermatitis. Curr Opin Immunol. 2002;12: 641-646.
16 Wollenberg A, Kraft S, Oppel T. Atopic dermatitis : pathogenetic mechanism. Clin Exp Dermatol. 2000;25:530-534.   DOI   ScienceOn
17 尙志鈞 輯校. 新修本草. 合肥:安徽科學技術出版社. 1981:292-295.
18 Finlay AY. Quality of life in atopic dermatitis. J Am Acad Dermatol. 2001;45:64-66.   DOI   ScienceOn
19 Robert Sidbury MD, Jon M. Hanifin MD. Old, New, And Emerging Therapies for Atopic Dermatitis. Dermatologic Clinics. 2000;18(1):1-11.
20 Schreiber SL, Crabtree GR. The mechanism of action of cyclosporin A and FK506. Immunol Today. 1992;13(4):136-142.   DOI   ScienceOn
21 陳貴延 主編. 本草綱目 通釋. 北京:學苑出版社. 1992:1374-1378.
22 張山雷 原著. 安世永, 金純一 編譯. 本草正義. 서울:청홍출판사. 2009:340.
23 Leung DYM. Pathogenesis of atopic dermatitis. J Allergy Clin Immunol. 1999;104:99-108.   DOI   ScienceOn
24 Aversa G, Punnonen, Cocks BG, de Waal Malefyt R, Vega F Jr, Zurawski SM, Zurawski G, de Vries JE. An IL-4 mutant protein inhibits both IL-4 or IL-3-induced human IgG4 and IgE synthesis and B cell proliferation : support for common component shared by IL-4 and IL-13 receptors. J Exp Med. 1993;178: 2213-2218.   DOI
25 대한피부과학회 교과서 편찬위원회. 피부과학 제5판. 서울:여문각. 2008:170-171.
26 Park YL, Park J. Pathogenesis of Atopic Dermatitis. Korean Journal of Investigative Dermatology. 2007;14(3):67-72.(Korean)
27 Horsmanheimo L, Harvima IT, Jarvikallio A, Harvima RJ, Naukkarinen A, Horsmanheimo M. Mast cells are one major source of interleukin-4 in atopic dermatitis. Br J Dermatol. 1994;131:348-353.   DOI   ScienceOn
28 Lee HR. Atopic Dermatitis. The Journal of Korean Oriental Pediatrics. 2000;43(9):1161-1167.(Korean)
29 Sandstrom MH, Faergemann J. Prognosis and prognostic factors in adult patient with atopic dermatitis : a long-term follow-up qeustionnaire study. Br J Dermatol. 2004;150:103-110.   DOI   ScienceOn
30 Patrick D. Does atopic dermatitis result from cytokine dysregulation?. J invest Dermatol. 1994;92:44-47.
31 顧伯華. 實用中醫外科學. 上海科學技術出版社. 1985:280-282.
32 전국한의과대학 공동교재편찬위원회. 본초학. 서울:영림사. 2005:245-247.
33 전국한의과대학 사상의학교실. 사상의학. 서울:집문당. 2005:289-291, 351.
34 Hwang GJ. Anti-inflammatory Effect of Taraxaci Herba Water Extract on Lipopolysaccharide-actived RAW 264.7 Macrophages. Graduate school of Kyungwon University master's thesis. 2011:1-29. (Korean)
35 Kim SK, Song HJ. Effects of Herba Taraxaci Extract on the Antialgesia and Antiinflammatory. The Journal of Won Kwang Oriental Medicine. 1992;23:152-161. (Korean)