Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Methanol Extract of Zizyphi Spinosi Semen Augments Pentobarbital-Induced Sleep through the Modification of GABAergic Systems

  • Hu, Zhenzhen (College of Pharmac, Chungbuk National University) ;
  • Kim, Chung-Soo (College of Pharmac, Chungbuk National University) ;
  • Oh, Eun-Hye (Department of Biochemistry, College of Medicine, Chungbuk National University) ;
  • Lee, Mi-Kyung (College of Pharmacy, Woosuk University) ;
  • Eun, Jae-Soon (College of Pharmacy, Woosuk University) ;
  • Hong, Jin-Tae (College of Pharmac, Chungbuk National University) ;
  • Oh, Ki-Wan (College of Pharmac, Chungbuk National University)
  • Received : 2012.02.04
  • Accepted : 2012.04.10
  • Published : 2012.06.30
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Abstract

Zizyphi Spinosi Semen (ZSS) have been widely used for the treatment of insomnia in Asia. This experiment was performed to investigate whether methanol extract of ZSS (MEZSS) has hypnotic effects through the ${\gamma}$-amino butyric acid (GABA)ergic systems. MEZSS inhibited the locomotor activity. MEZSS enhanced pentobarbital-induced sleep behaviors. However, MEZSS itself did not induce sleep at higher dose, similar to muscimol. On the other hand, both pentobarbital and MEZSS increased the non rapid eye move (NREM) sleep, especially reducing the -wave electroencephalogram (EEG) activity in REM sleep. MEZSS showed similar effects with muscimol on potentiating chloride influx induced by pentobarbital. MEZSS significantly increased GABAA receptors ${\gamma}$-subunit expression and slightly decreased ${\beta}$-subunit expression in hypothalamus and thalamus, showing that subunit-expression was similar to diazepam. In addition, MEZSS enhanced the expression of glutamic acid decarboxylase (GAD). In conclusion, it is suggested that MEZSS might augment pentobarbital-induced sleep behaviors through the modification of GABAergic systems.

Keywords

References

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