농약과학회지 (The Korean Journal of Pesticide Science)

  • 제16권1호
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  • Pages.62-68
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  • 2012
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  • 1226-6183(pISSN)
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  • 2287-2051(eISSN)
한국농약과학회 (The Korean Society of Pesticide Science)
권호 표지
DOI QR코드

DOI QR Code

미꾸리의 생물지표를 이용한 농업지역의 수생태계 건강성 스크리닝 평가(사례연구)

The Ecological Health Screening Assessment of Agricultural area using Biomarkers and Bioindicators in Misgurnus Anguillicaudatus (case study)

  • 김자현 (안전성평가연구소 환경독성연구센터) ;
  • 한선영 (안전성평가연구소 환경독성연구센터) ;
  • 염동혁 (안전성평가연구소 환경독성연구센터)
  • Kim, Ja-Hyun (Environmental Toxicology Research Center, Korea Institute of Toxicology) ;
  • Han, Sun-Young (Environmental Toxicology Research Center, Korea Institute of Toxicology) ;
  • Yeom, Dong-Hyuk (Environmental Toxicology Research Center, Korea Institute of Toxicology)
  • 투고 : 2011.12.22
  • 심사 : 2012.02.16
  • 발행 : 2012.03.31
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초록

본 연구에서는 내분비계 장애물질의 영향 및 생태계 건강성의 스크리닝 평가를 위해 미꾸리를 지표종으로 선정하여 대조지점(RA)과 농업지점(AS)에서 biomarker 4가지 항목과 bioindicator 4가지 항목을 포함하여 총 8가지 항목의 생물지표를 분석하였다. 생태계 건강성 평가를 위한 생물지표의 분석 결과 독성 화학물질의 영향을 나타내는 ethoxyresorufin-O-deethylase(EROD), acetylcholinesterase(AChE) 및 DNA damage 등의 생화학적 생물지표는 대조지점(RA)에 비해 유의한 차이가 있는 것으로 나타났다. 반면, 내분비계 장애물질의 영향을 반영하는 vitellogenin(VTG)는 농업지점(AS)에서 유의한 차이를 보이지 않았고, 개체수준의 생물지표(condition factor (CF), hepato-somatic index (HSI), gonado-somatic index (GSI)) 또한 유의한 차이를 보이지 않았다. 따라서 농업지점(AS)의 생태계 건강성은 내분비계 장애물질에 의한 영향은 미비한 것으로 판단되었으나, 농약, 중금속 등과 같은 독성을 보이는 화학물질이나 농업 지역의 특수성에 따른 서식지 환경 조건에 의해 일부 영향을 받은 것으로 사료되었다. 본 연구는 생화학적 생물지표와 개체수준의 생물지표를 이용한 스크리닝 평가로서 농업지역에 대한 종합적인 생태계 건강성 평가를 위해서는 생물지표 적용 항목을 확대하고, 개체군이나 군집 수준의 분석이 추가적으로 적용 되어야 하며 수생태계 내의 추정 오염물질에 대한 분석이 필요할 것으로 사료된다.

This study evaluated the screening level assessment of ecological health using four biomarkers and four bioindicators of Misgurnus anguillicaudatus as a indicator species in agricultural area of South Korea during May-June 2011. The endocrine disrupting chemical (EDC) indicators, such as vitellogenin (VTG) and gonado-somatic index (GSI), were not significantly changed in the agricultural site (p>0.05), indicating no effects. The biomarkers and bioindicators were compared between two sites of reference site (RS) and the agricultural site (AS) for screening assessment of ecological health. The ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, and DNA damage were significantly changed in the AS compared with the RS (p<0.05). But the individual level bioindicators such as condition factor (CF), hepato-somatic index (HSI), and gonado-somatic index (GSI) were not significantly different from reference site (RS). These results may indicate impairments of ecological health by toxic chemicals and environmental conditions. Current this study is based on screening assessment of biochemical and individual level biomarkers and bioindicators, so further study is required additional biomarkers and population or community level bioindicators for more specific health assessments in agricultural areas.

키워드

참고문헌

  1. Adams, S.M., and R.B. McLean (1985) Estimation of largemouth bass, Micropterus salmoides Lacepede, growth using the liver somatic index and physiological variables. J. Fish. Biol. 26:111-126. https://doi.org/10.1111/j.1095-8649.1985.tb04248.x
  2. Adams, S.M., M.S. Bevelhimer, M.S. Greeley, D.A. Levine, and S.J. Teh (1999) Ecological risk assessment in a large river-reservoir: 6. bioindicators of fish population health. Environmental Toxicology and Chemistry 18:628-640.
  3. Albanis, T.A., D.G. Hela, T.M. Sakellarides, and I.K. Konstantinou (1998) Monitoring of pesticide residues and their metabolites in surface and underground waters of Imathia (N. Greece) by solid phase extraction disks and gas chromatography. J. Chromatogr. A 824:59-71.
  4. Armin, S., T.S. Radau, T. Hahn, and R. Schulz (2007) Inhibition of rainbow trout acetylcholinesterase by aqueous and suspended particle associated organophosphorous insecticides. Chemosphere 68:605-612. https://doi.org/10.1016/j.chemosphere.2007.02.056
  5. Colborn, T., F.S. von Saal, and A.N. Soto (1993) Developmental effects of endocrine-disrupting chemicals in wildlife and humans. Environ. Health Perspect. 101:378-384. https://doi.org/10.1289/ehp.93101378
  6. Crews, D., E. Willingham, and J.K. Skipper (2000) Endocrine disrupters: present issues future direction. Quaterly Review of Biology 75:243-260. https://doi.org/10.1086/393498
  7. Denslow, N.D., M.C. Chow, K.J. Kroll, and L. Green (1999) Vitellogenin as a biomarker of exposure for estrogen or estrogen mimics. Ecotoxicology 8:385-398. https://doi.org/10.1023/A:1008986522208
  8. Dickerson, R.L., M.J. Hooper, and N.W. Gard (1994) Toxicological foundations of ecological risk assessment: Biomarker development and interpretation based on laboratory and wildlife species. Environ. Health Perspect. 102:65-69. https://doi.org/10.1289/ehp.94102s765
  9. Ellman, G.L., K.D. Courtney, V. Andres, Jr., and R.M. Featherstone (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology 7:88-90. https://doi.org/10.1016/0006-2952(61)90145-9
  10. Ham, K.D., S.M. Adams, M.J. Peterson (1997) Application of multiple bioindicators to differentiate spatial and temporal variability from the effects of contaminant exposure on fish. Ecotoxicology and Environmental safety 37:53-61. https://doi.org/10.1006/eesa.1997.1522
  11. Hinck, J.E., V.S. Blazer, N.D. Denslow, K.R. Echols, T.S. Gross, T.W. May, P.J. Anderson, J.J. Coyle, and D.E. Tillitt (2007) Chemical contaminants, health indicators, and reproductive biomarker responses in fish from the Colorado River and its tributaries. Science of the Total Environment 378:376-402. https://doi.org/10.1016/j.scitotenv.2007.02.032
  12. Jobling, S., M. Nolan, C.R. Tyler, G. Brighty, and J.P. Sumpter (1998) Widespread sexual disruption in wild fish. Environ. Sci. Technol. 32:2498-2506. https://doi.org/10.1021/es9710870
  13. Jung, J.H. R.F. Addison, and W.J. Shim (2007) Characterization of cholinesterases in marbled sole, Limanda yokohamae, and their inhibition in vitro by the fungicide iprobenfos. Marine Environmental Research 63:471-478. https://doi.org/10.1016/j.marenvres.2006.12.007
  14. Kennedy, S.W., and S.P. Jones (1994) Simultaneous measurement of cytochrome P4501A catalytic activity and total concentration with a fluorescence plate reader. Anal. Biochem. 222:217-223. https://doi.org/10.1006/abio.1994.1476
  15. Kim, G.B., R.F. Lee, and D.L. Mitchell (2000) Damage to grass shrimp (Palaemonetes pugio) embryo DNA by summer sunlight followed by DNA repair in the dark. Marine Biology 137: 675-682. https://doi.org/10.1007/s002270000371
  16. Kim, J.H., and D.H. Yeom (2009) Population response of Pale Chub (Zacco platypus) exposed to wastewater effluents in Gap Stream. Toxicology and Environmental Health Sciences 1:169-175. https://doi.org/10.1007/BF03216481
  17. Kim, J.H., D.H. Yeom, J.H. Kim and K.G. An (2012) New ecological health assessment approaches of an urban stream using molecular and physiological level biomarkers and bioindicators. Animal cells and systems DOI:10.1080/19768354.2011.653830.
  18. Lee, R.F., and G.B. Kim (2002) Comet assays to assess DNA damage and repair in grass shrimp embryos exposed to phototoxicants. Marine Environmental Research 54:465-469. https://doi.org/10.1016/S0141-1136(02)00128-9
  19. OECD (2009) OECD guideline for the testing of chemicals: "21-day fish assay: a short-term screening for oestrogenic and androgenic activity, and aromatase inhibition.
  20. Rankin, E.T., R. Miltner, C. Yoder, and D. Mishne (1999) Association between nutrients, habitat, and the aquatic biota in Ohio rivers and streams, Ohio EPA Technical bulletin MAS/1999-1, Ohio EPA, Columbus, USA.
  21. Richard, F.L., and S. Steinert (2003) Use of the single cell gel electrophoresis/ comet assay for detecting DNA damage in aquatic (marine and freshwater) animals. Mutation Research 544:43-64. https://doi.org/10.1016/S1383-5742(03)00017-6
  22. Singh, N.P., M.T. McCoy, R.R. Tice, and E.L. Schneider (1988) A simple technique for quantification of low levels of DNA damage in individual cells. Exp. Cell Res. 175:184 -191. https://doi.org/10.1016/0014-4827(88)90265-0
  23. USGS (2000) Biomonitoring of Environmental Status and Trends (BEST) Program: selected methods for monitoring chemical contaminants and their effects in aquatic ecosystems. USGS/BRD/ITR-2000-0005.
  24. Yeom, D.H., S.A. Lee, G.S. Kang, J. Seo, and S.K. Lee (2007) Stressor identification and health assessment of fish exposed to wastewater effluents in Miho Stream, South Korea. Chemosphere 67:2282-2292. https://doi.org/10.1016/j.chemosphere.2006.09.071
  25. 김상수, 이필용 (2001) 내분비계장애물질의 관리대책. 농업생명과학회지 8:13-21.
  26. 농촌진흥청 (2010) 농촌진흥청 고시 제2010-29호 농약의 등록기준 (2010. 10. 13.)
  27. 박인석, 김봉석, 임재현, 박효민, 남윤권, 정창화, 김동수 (1997) 미꾸리, Misgurnus anguillicaudatus의 생존율 향상을 위한 역교배체 생산. 한국양식학회지 10:363-371.
  28. 서용찬, 전은영, 김기동 (2005) 송천과 강릉 남대천수계의 내분비계 장애물질 조사 연구. 한국환경분석학회지 8:91-94.
  29. 안광국, 정승현, 최신석 (2001) 생물보전지수 (Index of Biological Integrity) 및 서식지평가지수 (Qualitative Habitat Evaluation Index)를 이용한 평창강의 수환경평가. 한국하천호수학회지 34:153-165.
  30. 이병천, 김수진, 윤준헌, 김은주, N.C. Duong, 엄익춘, F. Shiraishi, 최경희 (2010) 에스트로겐과 다이옥신 수용체 효모를 이용한 내분비계장애 영향 평가 한국환경분석학회지 13:109-115.
  31. 이은영, 노현호, 박영순, 강경원, 조성용, 이승열, 박인영, 김태화, 진용덕, 경기성 (2008) 청주 및 전주지역 유통 농산물 중 잔류농약 모니터링. 농약과학회지 12:357-362.
  32. 이지호, 박병준, 김진경, 김원일, 홍수명, 임건재, 홍무기 (2011) 주요 하천수역에서 검출된 농약의 수서생물에 대한 위해성 평가. 농약과학회지 15:48-54.
  33. 이철우, 최경희, 정석원, 김혜림, 서영록 (2009) 내분비계장애물질에 대한 이해와 미래 연구방향. 대한내분비학회지 24:7-14.
  34. 하광태, 박성규, 조태희, 한창호, 김성단, 이경아, 김시정, 장정임, 조한빈, 최병현 (2009) 서울 강북지역 유통 농산물의 내분비계장애 추정농약의 잔류실태(2007). 한국식품위생안전성학회지 24:136-142.

피인용 문헌

  1. Influence of Farming Practices on Weight-Length Relationship of the Loach (Misgurnus anguillicaudatus) Population in Rice Paddy Fields vol.35, pp.4, 2017, https://doi.org/10.11626/KJEB.2017.35.4.446
  2. Investigation of Korean Native Organisms for Development of Ecotoxicity Test : (1) Aquatic Test Species vol.40, pp.1, 2018, https://doi.org/10.4491/KSEE.2018.40.1.34